Rice line 1892S is an elite thermo-sensitive genic male sterile(TGMS)line for two-line hybrid rice production.However,1892S is susceptible to rice blast,bacterial blight and submergence.Here we reported the introducti...Rice line 1892S is an elite thermo-sensitive genic male sterile(TGMS)line for two-line hybrid rice production.However,1892S is susceptible to rice blast,bacterial blight and submergence.Here we reported the introduction of blast resistance(R)gene Pi9,bacterial blight R gene Xa21 and submergence tolerance gene Sub1A into 1892S genetic background through backcrossing and marker-assisted selection.The improved TGMS line 31892S and its hybrids conferred disease resistance to rice blast and bacterial blight,and showed submergence tolerance for over 14 d without significant loss of viability.The sterility-fertility conversion of 31892S was similar to that of 1892S.31892S and its derived hybrid rice had similar agronomic traits and grain quality with 1892S and the control hybrid rice,respectively.The newly developed 31892S provided an improved TGMS line for two-line hybrid rice production with disease resistance to rice blast and bacterial blight,and submergence tolerance with no yield penalty or change in grain quality.展开更多
Anthocyanins are a group of flavonoids that are found ubiquitously in fruits and vegetables.They act as antioxidants in plants and are considered to have potential benefits to human health.The tomato cultivar'Indi...Anthocyanins are a group of flavonoids that are found ubiquitously in fruits and vegetables.They act as antioxidants in plants and are considered to have potential benefits to human health.The tomato cultivar'Indigo Rose'produces purple-skinned fruits that are rich in anthocyanins in fruit peel.The current study focussed on the detection of anthocyanins and examined the expression profiling of anthocyanin biosynthetic and regulatory genes in Indigo Rose fruits grown under semi-closed greenhouse and fully controlled growth chamber conditions.The anthocyanin content in Indigo Rose fruits grown under growth chamber conditions with a long-day photoperiod and ambient temperature was higher than those grown in the greenhouse with a short-day photoperiod and high temperature.Under indoor growth conditions,moderate to high intensity of artificial light(160−240μmol m^(−2) s^(−1))and relatively low temperature(20−25℃)promoted anthocyanin production and accumulation in Indigo Rose fruits.The comparative studies on gene expression under different growth conditions also demonstrate that the expression levels of the structural and regulatory genes in the anthocyanin biosynthetic pathway have a positive correlation with the anthocyanin content in the fruit peel.The growth conditions identified in this study could be used to grow Indigo Rose in indoor farming in Singapore,where the tropical climate makes it challenging to set up a tomato plantation in an open field or greenhouse.展开更多
文摘Rice line 1892S is an elite thermo-sensitive genic male sterile(TGMS)line for two-line hybrid rice production.However,1892S is susceptible to rice blast,bacterial blight and submergence.Here we reported the introduction of blast resistance(R)gene Pi9,bacterial blight R gene Xa21 and submergence tolerance gene Sub1A into 1892S genetic background through backcrossing and marker-assisted selection.The improved TGMS line 31892S and its hybrids conferred disease resistance to rice blast and bacterial blight,and showed submergence tolerance for over 14 d without significant loss of viability.The sterility-fertility conversion of 31892S was similar to that of 1892S.31892S and its derived hybrid rice had similar agronomic traits and grain quality with 1892S and the control hybrid rice,respectively.The newly developed 31892S provided an improved TGMS line for two-line hybrid rice production with disease resistance to rice blast and bacterial blight,and submergence tolerance with no yield penalty or change in grain quality.
基金supported by the Agency for Science,Technology and Research (A*STAR)of the Ministry of Trade and Industry of Singapore under its Industry Alignment Fund Prepositioning (IAF-PP)with Grant No.A19E4a0101.
文摘Anthocyanins are a group of flavonoids that are found ubiquitously in fruits and vegetables.They act as antioxidants in plants and are considered to have potential benefits to human health.The tomato cultivar'Indigo Rose'produces purple-skinned fruits that are rich in anthocyanins in fruit peel.The current study focussed on the detection of anthocyanins and examined the expression profiling of anthocyanin biosynthetic and regulatory genes in Indigo Rose fruits grown under semi-closed greenhouse and fully controlled growth chamber conditions.The anthocyanin content in Indigo Rose fruits grown under growth chamber conditions with a long-day photoperiod and ambient temperature was higher than those grown in the greenhouse with a short-day photoperiod and high temperature.Under indoor growth conditions,moderate to high intensity of artificial light(160−240μmol m^(−2) s^(−1))and relatively low temperature(20−25℃)promoted anthocyanin production and accumulation in Indigo Rose fruits.The comparative studies on gene expression under different growth conditions also demonstrate that the expression levels of the structural and regulatory genes in the anthocyanin biosynthetic pathway have a positive correlation with the anthocyanin content in the fruit peel.The growth conditions identified in this study could be used to grow Indigo Rose in indoor farming in Singapore,where the tropical climate makes it challenging to set up a tomato plantation in an open field or greenhouse.
