Previously,we developed a gene delivery system using Bubble liposomes(perfluoropropane gas-entrapping liposomes;BLs)and ultrasound(US)[1].The combination of BLs and US can deliver plasmid DNA into the cytoplasm via th...Previously,we developed a gene delivery system using Bubble liposomes(perfluoropropane gas-entrapping liposomes;BLs)and ultrasound(US)[1].The combination of BLs and US can deliver plasmid DNA into the cytoplasm via the formation of transient membrane pores by cavitation.To enhance the efficiency of gene delivery,it is important to induce the cavitation of BLs at the vicinity of cells.In brief,cell-binding BLs would be useful for gene delivery to induce the cavitation on the cell membrane.展开更多
Gene therapy is applied into cardiovascular diseases,cancer and diseases that are due to genomic causes.Viral vectors are efficient carriers of genes for transduction,but some problems have become evident.Delivery vec...Gene therapy is applied into cardiovascular diseases,cancer and diseases that are due to genomic causes.Viral vectors are efficient carriers of genes for transduction,but some problems have become evident.Delivery vectors that are highly potent in terms of gene transduction efficiency should also be safe and easy to apply.Non-viral vectors have recently received focus as gene carriers,but their transduction efficiency is very low and not suitable for in vivo gene delivery.In addition,it is important to develop tissue-specific or selective gene delivery system to avoid side effects in gene therapy.展开更多
文摘Previously,we developed a gene delivery system using Bubble liposomes(perfluoropropane gas-entrapping liposomes;BLs)and ultrasound(US)[1].The combination of BLs and US can deliver plasmid DNA into the cytoplasm via the formation of transient membrane pores by cavitation.To enhance the efficiency of gene delivery,it is important to induce the cavitation of BLs at the vicinity of cells.In brief,cell-binding BLs would be useful for gene delivery to induce the cavitation on the cell membrane.
文摘Gene therapy is applied into cardiovascular diseases,cancer and diseases that are due to genomic causes.Viral vectors are efficient carriers of genes for transduction,but some problems have become evident.Delivery vectors that are highly potent in terms of gene transduction efficiency should also be safe and easy to apply.Non-viral vectors have recently received focus as gene carriers,but their transduction efficiency is very low and not suitable for in vivo gene delivery.In addition,it is important to develop tissue-specific or selective gene delivery system to avoid side effects in gene therapy.
