期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
细菌卤代烷烃脱卤酶基因在拟南芥菜中的高效表达 被引量:3
1
作者 郝林 john mundy 《应用生态学报》 CAS CSCD 1999年第1期83-85,共3页
报道了细菌Xanthobacterautotrophicus编码卤代烷烃脱卤酶基因在拟南芥菜中的高效表达.以土壤农杆菌介导将该基因整合到拟南芥菜基因组中,经数代筛选得到了转基因纯合种子,Northern印迹和气相色谱... 报道了细菌Xanthobacterautotrophicus编码卤代烷烃脱卤酶基因在拟南芥菜中的高效表达.以土壤农杆菌介导将该基因整合到拟南芥菜基因组中,经数代筛选得到了转基因纯合种子,Northern印迹和气相色谱检测表明,转基因的表达程度很高,酶量占细胞总可溶性蛋白的8%,酶活力达7.8mU·ml-1提取物.转基因植株在含二氯乙烷的培养基上不能生长. 展开更多
关键词 拟南芥菜 基因表达 土壤农杆菌 chlA基因
下载PDF
Gene Discovery and Functional Analyses in the Model Plant Arabidopsis 被引量:3
2
作者 Cai-Ping Feng john mundy 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第1期5-14,共10页
The present mini-review describes newer methods and strategies, including transposon and T-DNA insertions, TILLING, Deleteagene, and RNA interference, to functionally analyze genes of interest in the model plant Arabi... The present mini-review describes newer methods and strategies, including transposon and T-DNA insertions, TILLING, Deleteagene, and RNA interference, to functionally analyze genes of interest in the model plant Arabidopsis. The relative advantages and disadvantages of the systems are also discussed. 展开更多
关键词 ARABIDOPSIS functional genomics MUTAGENESIS reverse genetics.
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部