Araucaria angustifolia(Bertol.)Kuntze is a representative species of the Mixed Ombrophilous Forest in the Atlantic Forest Biome of Brazil.The development of a germplasm conservation protocol for long-term seed bank st...Araucaria angustifolia(Bertol.)Kuntze is a representative species of the Mixed Ombrophilous Forest in the Atlantic Forest Biome of Brazil.The development of a germplasm conservation protocol for long-term seed bank storage is compromised for this species,as it is sensitive to desiccation.Furthermore,in situ establishment of a soil seed bank in its natural habitat may be limited.This study evaluates the storability of two provenances of A.angustifolia seeds and their behavior in an artificial soil seed bank in two forest environments(understory and edge).Results show that both seed provenances may be stored at 5C for approximately 12 months,retaining high viability.The subsequent decrease in germination was associated with a reduction and an increase in seed water content,as well as with increased electrical conductivity.In the understory environment,seed viability was above 85%for the first 60 days,and at the end of the experiment(270 days),seedlings emerged.However,at the forest edge,there was a total loss of seed viability after 120 days associated with a reduction in water content and high predation.It is concluded,therefore,that short-term storage of A.angustifolia seeds is possible in a cold room,which is fundamental to supply seed demand outside the production period.Forest cover conservation is important for regeneration and conservation of the species.展开更多
基金The authors thank the Coordination for the Improvement of Higher Level Personnel(CAPES,Brazil)for granting the main author’s scholarship,the Brazilian Institute of the Environment and Renewable Natural Resources(IBAMA)for authorizing seed collection in the different locations(License No 34243-3),the FLONA(Sa˜o Francisco de Paula/RS)for help in seed collection of RS provenance,and to anonymous reviewers for valuable contributions.
文摘Araucaria angustifolia(Bertol.)Kuntze is a representative species of the Mixed Ombrophilous Forest in the Atlantic Forest Biome of Brazil.The development of a germplasm conservation protocol for long-term seed bank storage is compromised for this species,as it is sensitive to desiccation.Furthermore,in situ establishment of a soil seed bank in its natural habitat may be limited.This study evaluates the storability of two provenances of A.angustifolia seeds and their behavior in an artificial soil seed bank in two forest environments(understory and edge).Results show that both seed provenances may be stored at 5C for approximately 12 months,retaining high viability.The subsequent decrease in germination was associated with a reduction and an increase in seed water content,as well as with increased electrical conductivity.In the understory environment,seed viability was above 85%for the first 60 days,and at the end of the experiment(270 days),seedlings emerged.However,at the forest edge,there was a total loss of seed viability after 120 days associated with a reduction in water content and high predation.It is concluded,therefore,that short-term storage of A.angustifolia seeds is possible in a cold room,which is fundamental to supply seed demand outside the production period.Forest cover conservation is important for regeneration and conservation of the species.
