Sensitivity and specificity of five commonly used rapid malaria test kits in South-South geopolitical zone of Nigeria were analyzed, namely Plasmotest (Biotech International, Germany), Malariatest (Acon Diagnostic, US...Sensitivity and specificity of five commonly used rapid malaria test kits in South-South geopolitical zone of Nigeria were analyzed, namely Plasmotest (Biotech International, Germany), Malariatest (Acon Diagnostic, USA), AcumPF (Acumen, China), PF test (Diaspot, Indonesia) and Malaria P.f. (Global, USA). A total of 200 positive malaria blood sample using microscopy as gold standard and 200 negative were obtained from adult and children. All the kits were tested against these gold standards as directed by the manufacturers. Of the 100 positive gold standard used from children sample, 96% sensitivity were recorded for Biotech, Acon and Global 97%, while Acumen and Diaspot had 99% and 98% respectively. The the highest Specificity was recorded with Biotech 99% both Acumen and Diaspot had 97% specificity. In adult blood sample, there was general reduction in the sensitivity of the test kits. We obtained Sensitivity of 30% with Biotech, 46% with Acon, Global 73%, Diaspot 75%, and Acumen 78%. Considering the increasing influx of MRDT’s into the country, and from the result obtained from this study, there is need for control agensis to ensure that diagnostic test kits be reevaluated before introduction into Nigeria market.展开更多
Anemia in children is defined by the World Health Organization as a hemoglobin concentration below 11 g/dl for children (0.5-5.0 yrs) and12 g/dl for teens (12-15 yrs). 4 ml of venous blood sample was collected in EDTA...Anemia in children is defined by the World Health Organization as a hemoglobin concentration below 11 g/dl for children (0.5-5.0 yrs) and12 g/dl for teens (12-15 yrs). 4 ml of venous blood sample was collected in EDTA container. Of the total of three hundred and thirty four (334) subjects, one hundred and fifty two (152) were Females and one hundred and eighty two (182) were Males. Intestinal parasite assessment was done by Direct Smear technique and Formol-Ether concentration methods. Hemoglobin concentration was analyzed using Cyanmethaemoglobin method. Thirty (30) subjects were infested with Ascaris lumbricoides (single infestation), Ninety Five (95) subjects were infested with Ascaris lumbricoides and Hookworm (Double infestation) and Forty Two (42) subjects were infested with Ascaris lumbricoides, Hookworm, Entamoeba histolytica and Trichuris trichiura (Multiple infestation). The Mean ± Standard Deviation of Hemoglobin concentration of the various infestation types against the control subject shows a statistically significant decrease展开更多
C reactive protein is sensitive physiological biomarkers of sub clinical inflammation associated with hyperglycemia. The aim of this study is to determine the fasting serum C reactive protein level in type 2 diabetes ...C reactive protein is sensitive physiological biomarkers of sub clinical inflammation associated with hyperglycemia. The aim of this study is to determine the fasting serum C reactive protein level in type 2 diabetes mellitus patients attending diabetic clinic in Benin City, Nigeria. The population sample consists of 142 subjects. 71 patients were known type 2 diabetes mellitus, while the other 71 were age matched control subjects. Fasting glucose and C reactive protein were estimated using glucose oxidase method and ELISA method respectively. The age group that has the highest number of type 2 diabetes mellitus was 41 - 50 (64% of males and 36% of the females). Our finding revealed that C reactive protein and serum glucose level of type 2 Diabetes mellitus in both females and males show a statistically significant increase as compared with age matched Control subjects,展开更多
文摘Sensitivity and specificity of five commonly used rapid malaria test kits in South-South geopolitical zone of Nigeria were analyzed, namely Plasmotest (Biotech International, Germany), Malariatest (Acon Diagnostic, USA), AcumPF (Acumen, China), PF test (Diaspot, Indonesia) and Malaria P.f. (Global, USA). A total of 200 positive malaria blood sample using microscopy as gold standard and 200 negative were obtained from adult and children. All the kits were tested against these gold standards as directed by the manufacturers. Of the 100 positive gold standard used from children sample, 96% sensitivity were recorded for Biotech, Acon and Global 97%, while Acumen and Diaspot had 99% and 98% respectively. The the highest Specificity was recorded with Biotech 99% both Acumen and Diaspot had 97% specificity. In adult blood sample, there was general reduction in the sensitivity of the test kits. We obtained Sensitivity of 30% with Biotech, 46% with Acon, Global 73%, Diaspot 75%, and Acumen 78%. Considering the increasing influx of MRDT’s into the country, and from the result obtained from this study, there is need for control agensis to ensure that diagnostic test kits be reevaluated before introduction into Nigeria market.
文摘Anemia in children is defined by the World Health Organization as a hemoglobin concentration below 11 g/dl for children (0.5-5.0 yrs) and12 g/dl for teens (12-15 yrs). 4 ml of venous blood sample was collected in EDTA container. Of the total of three hundred and thirty four (334) subjects, one hundred and fifty two (152) were Females and one hundred and eighty two (182) were Males. Intestinal parasite assessment was done by Direct Smear technique and Formol-Ether concentration methods. Hemoglobin concentration was analyzed using Cyanmethaemoglobin method. Thirty (30) subjects were infested with Ascaris lumbricoides (single infestation), Ninety Five (95) subjects were infested with Ascaris lumbricoides and Hookworm (Double infestation) and Forty Two (42) subjects were infested with Ascaris lumbricoides, Hookworm, Entamoeba histolytica and Trichuris trichiura (Multiple infestation). The Mean ± Standard Deviation of Hemoglobin concentration of the various infestation types against the control subject shows a statistically significant decrease
文摘C reactive protein is sensitive physiological biomarkers of sub clinical inflammation associated with hyperglycemia. The aim of this study is to determine the fasting serum C reactive protein level in type 2 diabetes mellitus patients attending diabetic clinic in Benin City, Nigeria. The population sample consists of 142 subjects. 71 patients were known type 2 diabetes mellitus, while the other 71 were age matched control subjects. Fasting glucose and C reactive protein were estimated using glucose oxidase method and ELISA method respectively. The age group that has the highest number of type 2 diabetes mellitus was 41 - 50 (64% of males and 36% of the females). Our finding revealed that C reactive protein and serum glucose level of type 2 Diabetes mellitus in both females and males show a statistically significant increase as compared with age matched Control subjects,
