A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids,which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The ...A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids,which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells.Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.展开更多
The modification effect of CX-type (CX means the modifiers that have longeffective term) modifiers applied in ZL108 and ZL104 Al-Si alloys has been studied in detail. Theresults show that the morphologies of the eutec...The modification effect of CX-type (CX means the modifiers that have longeffective term) modifiers applied in ZL108 and ZL104 Al-Si alloys has been studied in detail. Theresults show that the morphologies of the eutectic silicon and the primary silicon can be modifiedand refined simultaneously. The modification effect acts quickly and can maintain a period of 8 h bythe CX-type modifiers. The CX-type modifiers increase the mechanical properties of Al-Si alloys andimprove the service properties of machine parts made of Al-Si alloys (such as piston and cylinderetc.). In modifying processes, the amount of the addition of CX-type modifiers is smaller than thatof any other modifiers, and the modifying procedures are simple. There are no smoke, no dust, and noirritant smell in modifying processes using CX-type modifiers. Therefore, the CX-type modifiershave advantages in economy and environment protection.展开更多
基金This study was supported by grants from National Natural Science Foundation of China(No.30240019,30300060)National High Technology Research and Development Program of China(No.2001 AA221I31)+1 种基金Major State Basic Research Project(No.Gl 999055901)State Ministry of Science and Technology Program(No.2002BA7llA0l).
文摘A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids,which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells.Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.
基金This work was supported by National Natural Science Foundation of China (No.59771011).
文摘The modification effect of CX-type (CX means the modifiers that have longeffective term) modifiers applied in ZL108 and ZL104 Al-Si alloys has been studied in detail. Theresults show that the morphologies of the eutectic silicon and the primary silicon can be modifiedand refined simultaneously. The modification effect acts quickly and can maintain a period of 8 h bythe CX-type modifiers. The CX-type modifiers increase the mechanical properties of Al-Si alloys andimprove the service properties of machine parts made of Al-Si alloys (such as piston and cylinderetc.). In modifying processes, the amount of the addition of CX-type modifiers is smaller than thatof any other modifiers, and the modifying procedures are simple. There are no smoke, no dust, and noirritant smell in modifying processes using CX-type modifiers. Therefore, the CX-type modifiershave advantages in economy and environment protection.
