It is widely known that intestinal capacities such as the enzymatic hydrolysis of carbohydrates,lipids and proteins,and the subsequent absorption of the hydrolyzed products.are evolutionary matched to dietary loads an...It is widely known that intestinal capacities such as the enzymatic hydrolysis of carbohydrates,lipids and proteins,and the subsequent absorption of the hydrolyzed products.are evolutionary matched to dietary loads and feeding behaviors.In this study,we demonstrate that the protein expression of apically located sodium-dependent glucose cotransporter-1(SGLT-1)throughout rat ontogeny is daily adjusted to afford glucose uptake when the load of this metabolically essential monosaccharide in the intestinal lumen is maximum.The jejunal expression of SGLT-1 protein in 14 one-day-old suckling pups was found to increase at dark and early light phase(P<0.05).when they have a better access to mother milk.In weaning21-d-old and juvenile 28-d-old rats,the cotransporter expression was high throughout the entire day(P<0.05).Finally,adult 90-d-old rats showed a well-developed circadian rhythm for SGLT-1 protein(P<0.05).whose expression increased at late light and dark phase when the highest intestinal glucose load was achieved.To our knowledge,these results are the first reporting the daily profile of SGLT-1 expression during rat early developmental stage and may contribute to understand the biological significance of a well-established molecular capacity to deal with the crucial increase of glucose load in the diet during the weaning process.展开更多
基金supported by PIP 2016-2018 by CONICET,PROICO 2-0814 and PROICO 2-0516 by CyT-UNSL and PICT-201-0595 by Agencia Nacional de Promocion Cientifica y Tecnologica
文摘It is widely known that intestinal capacities such as the enzymatic hydrolysis of carbohydrates,lipids and proteins,and the subsequent absorption of the hydrolyzed products.are evolutionary matched to dietary loads and feeding behaviors.In this study,we demonstrate that the protein expression of apically located sodium-dependent glucose cotransporter-1(SGLT-1)throughout rat ontogeny is daily adjusted to afford glucose uptake when the load of this metabolically essential monosaccharide in the intestinal lumen is maximum.The jejunal expression of SGLT-1 protein in 14 one-day-old suckling pups was found to increase at dark and early light phase(P<0.05).when they have a better access to mother milk.In weaning21-d-old and juvenile 28-d-old rats,the cotransporter expression was high throughout the entire day(P<0.05).Finally,adult 90-d-old rats showed a well-developed circadian rhythm for SGLT-1 protein(P<0.05).whose expression increased at late light and dark phase when the highest intestinal glucose load was achieved.To our knowledge,these results are the first reporting the daily profile of SGLT-1 expression during rat early developmental stage and may contribute to understand the biological significance of a well-established molecular capacity to deal with the crucial increase of glucose load in the diet during the weaning process.
