Reduced photosynthesis results directly from nitrogen or water deficiency in wheat plants,and leads to a decrease in grain yield.In this study,by measuring the effects of water and N deficiencies,both individually and...Reduced photosynthesis results directly from nitrogen or water deficiency in wheat plants,and leads to a decrease in grain yield.In this study,by measuring the effects of water and N deficiencies,both individually and combined,we characterized the responses of wheat(Triticum aestivum L.Yumai 49-198)plants to these two deficiencies using physiological measurements and comparative proteomics.Significant decreases in grain yield and leaf photosynthetic performance were observed in all deficiency conditions,and 106 photosynthetic proteins that showed responses were identified.Nitrogen deficiency induced the least change in photosynthetic proteins,and similar changes in most of these proteins were also observed for the combined nitrogen and water deficiencies.Water deficiency induced the largest change in photosynthetic proteins and resulted in the lowest 1000-kernel weight.Severe decreases in photosynthesis in both the water-deficiency and combined N and water deficiency groups were reflected mainly in an imbalanced ATP/NADPH ratio associated with the light reaction,which influences carbon metabolism in the Calvin cycle.Photorespiration was respectively stimulated or inhibited by N or water deficiency,while suppression of photorespiratory flux and activation of nitrogen recycling were observed in the combined N and water deficiency treatments.Comparison of photosynthetic proteins between experimental sites suggested that precipitation affected linear electron flow in the photoreaction,and thus photosynthetic efficiency.Our results provide a baseline for future studies of the roles of these photosynthetic proteins in the response to N or water deficiency and their effect on 1000-kernel weight.展开更多
Introduction: Spinal cord infarction (SCI) is a relatively rare disease. Abnormality on magnetic resonance imaging (MRI) is an important diagnostic criterion. It may be normal on the first day. Then abnormal signal wi...Introduction: Spinal cord infarction (SCI) is a relatively rare disease. Abnormality on magnetic resonance imaging (MRI) is an important diagnostic criterion. It may be normal on the first day. Then abnormal signal will appear a few days later. There were some reports with negative MRI findings at the beginning. However, those reports were neither found abnormalities on repeat MRI nor without repeat MRI. Case Presentation: We report a case of SCI presented as paralysis of lower limbs and chest pain with no abnormalities on MRI through the course of disease in a 74-year-old female. Anti-platelet aggregation drugs, stabilizing plaque treatments and rehabilitation exercise were performed. The patient recovered significantly. She could walk independently a month after the onset. Conclusions: Although MRI provides important evidence for diagnosis, clinical manifestations are the most important when diagnosing. For patients who were highly suspected of SCI clinically, treatment for vascular diseases should be applied as soon as possible even if no lesion is found on imaging examination.展开更多
Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness(AHR),inflammatory cell infiltration,and airway remodeling.F-box/WD repeat-containing protein 7(FBXW7),an E3 ubiquit...Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness(AHR),inflammatory cell infiltration,and airway remodeling.F-box/WD repeat-containing protein 7(FBXW7),an E3 ubiquitin ligase,is required for various endothelial functions,such as cell migration,inflammation,and endothelial integrity.This study aimed to investigate the role of FBXW7 in lipopolysaccharide(LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro.By using lentivirus-based technology,FBXW7 was overexpressed or silenced(24 h)in human bronchial epithelial(16HBE)cells,which were treated with LPS or not(24 h).Immunoprecipitation(IP)detection and Western blot analysis were used to evaluate the interaction of target proteins.Cell permeability was measured using transepithelial electrical resistance and FITC dextran flux(48 h).IL-1β,IL-18 and TNF-αin cell supernatants were measured using ELISA(48 h).The results showed that LPS stimulation suppressed FBXW7 expression in a time-and dose-dependent manner.LPS exposure decreased cell proliferation,elevated IL-1β,IL-18 and TNF-α,increased epithelial permeability,and p38 phosphorylation.These LPS-induced changes were partly compromised by FBXW7 overexpression.Similar to LPS stimulation,FBXW7 knockdown increased epithelial permeability and levels of inflammatory cytokines and p38 phosphorylation,which were,in part,blocked by apoptosis signal-regulating kinase(ASK)1 knockdown or p38 pathway inhibition.IP and Western blot analysis showed that FBXW7 interacted with ASK1.ASK1 expression was inversely associated with FBXW7 expression.FBXW7 overexpression markedly enhanced ASK1 ubiquitination.These data revealed that FBXW7 counter against inflammation and protects epithelial barrier integrity in bronchial epithelial cells by promoting ubiquitination-mediated degradation of ASK1 via the p38 pathway.展开更多
Objective:To study the effect of intrapleural endostatin and mannatide infusion on malignant molecule expression in pleural fluid of malignant pleural effusion.Methods:Patients with lung cancer and malignant pleural e...Objective:To study the effect of intrapleural endostatin and mannatide infusion on malignant molecule expression in pleural fluid of malignant pleural effusion.Methods:Patients with lung cancer and malignant pleural effusion treated in our hospital between April 2013 and December 2015 were selected and randomly divided into two groups, the observation group received intrapleural endostatin and mannatide infusion treatment and control group accepted routine intrapleural infusion treatment. 4 weeks after treatment, the pleural fluid samples were collected to determine the levels of tumor markers, invasion-related molecules, VEGF-related molecules and anti-tumor cytokines.Results:4 weeks after treatment, CEA, CYFRA21-1, NSE, SCC-Ag, CXCL12, CXCR4, MMP2, MMP9, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-R1, VEGF-R2 and VEGF-R3 levels in pleural fluid of observation group were significantly lower than those of control group while LASS2/TMSG-1, IFN-γ, IL-2, TNF-α, IL-17 and IL-23 levels were significantly higher than those of control group.Conclusion:Intrapleural endostatin and mannatide infusion treatment of malignant pleural effusion can more effectively kill cancer cells, inhibit cell invasion, angiogenesis and lymphangiogenesis, and enhance antitumor immune response.展开更多
A simple quasi-distributed fiber sensing interrogation system based on random speckles is proposed for weak fiber Bragg gratings(WFBGs) in this work. Without using tunable lasers or spectrometers, a piece of multimode...A simple quasi-distributed fiber sensing interrogation system based on random speckles is proposed for weak fiber Bragg gratings(WFBGs) in this work. Without using tunable lasers or spectrometers, a piece of multimode fiber is applied to interrogate the WFBGs relying on the wavelength sensitivity of speckles. Instead of the CCD sensor, an InGaAs quadrant detector serves as the receiver to capture the fast-changing speckle patterns. A supervised deep learning algorithm of the multilayer perceptron architecture is implemented to process speckle data and to interrogate temperature changes or dynamic strains. The proposed demodulation system is experimentally demonstrated for WFBGs with 0.1% reflectivity.The experimental results demonstrate that the new system is capable of measuring temperature change with an accuracy of 1℃ and achieving dynamic frequency of 100 Hz. This speckle-based interrogation system paves a new way for distributed WFBGs sensing with a simple design.展开更多
Inactivated COVID-19 vaccines have been widely used to vaccinate the Chinese population.However,limited literature exists to explore the effect of obesity on the humoral and cellular immune response to these vaccines....Inactivated COVID-19 vaccines have been widely used to vaccinate the Chinese population.However,limited literature exists to explore the effect of obesity on the humoral and cellular immune response to these vaccines.In this study,132 high BMI(Body mass index)(obesity and overweight,BMI≥24 kg/m^(2))and 82 normal BMI(BMI<24 kg/m^(2))participants were enrolled.Adverse events(AEs),Spike receptor-binding domain IgG antibody(anti-RBD-IgG),neutralizing antibodies(NAbs),and specific B-cell and T-cell responses were evaluated 21–105 days after full-course inactivated COVID-19 vaccination.The overall incidence of AEs was similar in individuals with and without obesity/overweight.No serious vaccine-related AEs occurred.Individuals with obesity/overweight had a reduced seropositivity rate of NAbs compared to those with normal BMI.Anti-RBD-IgG and NAbs titers in the high BMI group were significantly lower than those in the normal BMI group.The frequencies of RBD-specific memory B cells(MBCs)and the numbers of spike-specific TNF-α+spot-forming cells(SFCs)in individuals with obesity/overweight were reduced compared with those noted in individuals without obesity/overweight.A similar trend of weakened humoral responses was also observed in individuals with central obesity.Our study results suggested that inactivated COVID-19 vaccines were safe and well tolerated but induced poor humoral and cellular immune responses in Chinese individuals with obesity/overweight.展开更多
Polyneuropathy,organomegaly,endocrinopathy,M proteins,and skin changes(POEMS)syndrome is a multisystemic disorder that clinically manifests as paraneoplastic and monoclonal plasma cell dyscrasia.Its acronym is derived...Polyneuropathy,organomegaly,endocrinopathy,M proteins,and skin changes(POEMS)syndrome is a multisystemic disorder that clinically manifests as paraneoplastic and monoclonal plasma cell dyscrasia.Its acronym is derived from its principal characteristics:polyneuropathy,organomegaly,endocrinopathy,M proteins,and skin changes.Here,the authors reported a case of POEMS syndrome that was also associated with Castleman disease.A 53-year-old female patient was admitted to our hospital with limb weakness,numbness,edema,abdominal distention,and fever.Physical examination revealed tetraplegia,paraesthesia,and hyporeflexia in all four limbs,in addition to lymphadenectasis,splenomegaly,skin hyperpigmentation,hypertrichosis,and pitting edema.Laboratory tests and imaging revealed thrombocytosis,hypothyroidism,diabetes,hydropericardium,hydrothorax,splenomegaly,and lymphadenectasis.Electromyography showed the characteristic patterns of both demyelinating disease and axonal degeneration.Serum protein electrophoresis revealed monoclonal immunoglobulin G-lambda paraproteins.Histological examination clearly diagnosed the disease as the hyaline vascular subtype.The final diagnosis in this case was POEMS syndrome in association with Castleman disease.展开更多
基金supported by the National Key Research and Development Program of China(2018YFD0300707 and 2016YFD0300105)the Modern Wheat Industrial Technology System of Henan Province(S2010-01-G07).
文摘Reduced photosynthesis results directly from nitrogen or water deficiency in wheat plants,and leads to a decrease in grain yield.In this study,by measuring the effects of water and N deficiencies,both individually and combined,we characterized the responses of wheat(Triticum aestivum L.Yumai 49-198)plants to these two deficiencies using physiological measurements and comparative proteomics.Significant decreases in grain yield and leaf photosynthetic performance were observed in all deficiency conditions,and 106 photosynthetic proteins that showed responses were identified.Nitrogen deficiency induced the least change in photosynthetic proteins,and similar changes in most of these proteins were also observed for the combined nitrogen and water deficiencies.Water deficiency induced the largest change in photosynthetic proteins and resulted in the lowest 1000-kernel weight.Severe decreases in photosynthesis in both the water-deficiency and combined N and water deficiency groups were reflected mainly in an imbalanced ATP/NADPH ratio associated with the light reaction,which influences carbon metabolism in the Calvin cycle.Photorespiration was respectively stimulated or inhibited by N or water deficiency,while suppression of photorespiratory flux and activation of nitrogen recycling were observed in the combined N and water deficiency treatments.Comparison of photosynthetic proteins between experimental sites suggested that precipitation affected linear electron flow in the photoreaction,and thus photosynthetic efficiency.Our results provide a baseline for future studies of the roles of these photosynthetic proteins in the response to N or water deficiency and their effect on 1000-kernel weight.
文摘Introduction: Spinal cord infarction (SCI) is a relatively rare disease. Abnormality on magnetic resonance imaging (MRI) is an important diagnostic criterion. It may be normal on the first day. Then abnormal signal will appear a few days later. There were some reports with negative MRI findings at the beginning. However, those reports were neither found abnormalities on repeat MRI nor without repeat MRI. Case Presentation: We report a case of SCI presented as paralysis of lower limbs and chest pain with no abnormalities on MRI through the course of disease in a 74-year-old female. Anti-platelet aggregation drugs, stabilizing plaque treatments and rehabilitation exercise were performed. The patient recovered significantly. She could walk independently a month after the onset. Conclusions: Although MRI provides important evidence for diagnosis, clinical manifestations are the most important when diagnosing. For patients who were highly suspected of SCI clinically, treatment for vascular diseases should be applied as soon as possible even if no lesion is found on imaging examination.
基金funded by Fundamental research program funding of Ninth People’s Hospital Affiliated to Shanghai Jiaotong University School of Medicine(JYZZ069).
文摘Bronchial asthma is a common chronic inflammatory disease characterized by airway hyperresponsiveness(AHR),inflammatory cell infiltration,and airway remodeling.F-box/WD repeat-containing protein 7(FBXW7),an E3 ubiquitin ligase,is required for various endothelial functions,such as cell migration,inflammation,and endothelial integrity.This study aimed to investigate the role of FBXW7 in lipopolysaccharide(LPS)-induced epithelial barrier impairment in bronchial epithelial cells in vitro.By using lentivirus-based technology,FBXW7 was overexpressed or silenced(24 h)in human bronchial epithelial(16HBE)cells,which were treated with LPS or not(24 h).Immunoprecipitation(IP)detection and Western blot analysis were used to evaluate the interaction of target proteins.Cell permeability was measured using transepithelial electrical resistance and FITC dextran flux(48 h).IL-1β,IL-18 and TNF-αin cell supernatants were measured using ELISA(48 h).The results showed that LPS stimulation suppressed FBXW7 expression in a time-and dose-dependent manner.LPS exposure decreased cell proliferation,elevated IL-1β,IL-18 and TNF-α,increased epithelial permeability,and p38 phosphorylation.These LPS-induced changes were partly compromised by FBXW7 overexpression.Similar to LPS stimulation,FBXW7 knockdown increased epithelial permeability and levels of inflammatory cytokines and p38 phosphorylation,which were,in part,blocked by apoptosis signal-regulating kinase(ASK)1 knockdown or p38 pathway inhibition.IP and Western blot analysis showed that FBXW7 interacted with ASK1.ASK1 expression was inversely associated with FBXW7 expression.FBXW7 overexpression markedly enhanced ASK1 ubiquitination.These data revealed that FBXW7 counter against inflammation and protects epithelial barrier integrity in bronchial epithelial cells by promoting ubiquitination-mediated degradation of ASK1 via the p38 pathway.
文摘Objective:To study the effect of intrapleural endostatin and mannatide infusion on malignant molecule expression in pleural fluid of malignant pleural effusion.Methods:Patients with lung cancer and malignant pleural effusion treated in our hospital between April 2013 and December 2015 were selected and randomly divided into two groups, the observation group received intrapleural endostatin and mannatide infusion treatment and control group accepted routine intrapleural infusion treatment. 4 weeks after treatment, the pleural fluid samples were collected to determine the levels of tumor markers, invasion-related molecules, VEGF-related molecules and anti-tumor cytokines.Results:4 weeks after treatment, CEA, CYFRA21-1, NSE, SCC-Ag, CXCL12, CXCR4, MMP2, MMP9, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-R1, VEGF-R2 and VEGF-R3 levels in pleural fluid of observation group were significantly lower than those of control group while LASS2/TMSG-1, IFN-γ, IL-2, TNF-α, IL-17 and IL-23 levels were significantly higher than those of control group.Conclusion:Intrapleural endostatin and mannatide infusion treatment of malignant pleural effusion can more effectively kill cancer cells, inhibit cell invasion, angiogenesis and lymphangiogenesis, and enhance antitumor immune response.
基金supported by the National Key Research and Development Program of China(No.2021YFC3340400)Key Laboratory of Medical Electronics and Digital Health of Zhejiang Province(No.MEDH202209)+1 种基金Natural Science Foundation of Zhejiang Province(No.LY22F050004)Zhejiang Xinmiao Talents Program(No.2022R409043).
文摘A simple quasi-distributed fiber sensing interrogation system based on random speckles is proposed for weak fiber Bragg gratings(WFBGs) in this work. Without using tunable lasers or spectrometers, a piece of multimode fiber is applied to interrogate the WFBGs relying on the wavelength sensitivity of speckles. Instead of the CCD sensor, an InGaAs quadrant detector serves as the receiver to capture the fast-changing speckle patterns. A supervised deep learning algorithm of the multilayer perceptron architecture is implemented to process speckle data and to interrogate temperature changes or dynamic strains. The proposed demodulation system is experimentally demonstrated for WFBGs with 0.1% reflectivity.The experimental results demonstrate that the new system is capable of measuring temperature change with an accuracy of 1℃ and achieving dynamic frequency of 100 Hz. This speckle-based interrogation system paves a new way for distributed WFBGs sensing with a simple design.
基金supported by the National Science and Technology Major Project of China(No.2017ZX10202203-007,No.2017ZX10202203-008,and No.2018ZX10302206-003)a pilot project of clinical cooperation between traditional Chinese and western medicine for significant and complicated diseases of National Administration of Traditional Chinese Medicine:hepatic fibrosis.We also acknowledge the support of the National Natural Science Foundation of China(No.81772198)+1 种基金the Natural Science Foundation of Chongqing,China(No.cstc2020jcyj-msxmX0389)General Project of Chongqing Natural Science Foundation(No.cstc2020jcyj-msxmX0015).
文摘Inactivated COVID-19 vaccines have been widely used to vaccinate the Chinese population.However,limited literature exists to explore the effect of obesity on the humoral and cellular immune response to these vaccines.In this study,132 high BMI(Body mass index)(obesity and overweight,BMI≥24 kg/m^(2))and 82 normal BMI(BMI<24 kg/m^(2))participants were enrolled.Adverse events(AEs),Spike receptor-binding domain IgG antibody(anti-RBD-IgG),neutralizing antibodies(NAbs),and specific B-cell and T-cell responses were evaluated 21–105 days after full-course inactivated COVID-19 vaccination.The overall incidence of AEs was similar in individuals with and without obesity/overweight.No serious vaccine-related AEs occurred.Individuals with obesity/overweight had a reduced seropositivity rate of NAbs compared to those with normal BMI.Anti-RBD-IgG and NAbs titers in the high BMI group were significantly lower than those in the normal BMI group.The frequencies of RBD-specific memory B cells(MBCs)and the numbers of spike-specific TNF-α+spot-forming cells(SFCs)in individuals with obesity/overweight were reduced compared with those noted in individuals without obesity/overweight.A similar trend of weakened humoral responses was also observed in individuals with central obesity.Our study results suggested that inactivated COVID-19 vaccines were safe and well tolerated but induced poor humoral and cellular immune responses in Chinese individuals with obesity/overweight.
文摘Polyneuropathy,organomegaly,endocrinopathy,M proteins,and skin changes(POEMS)syndrome is a multisystemic disorder that clinically manifests as paraneoplastic and monoclonal plasma cell dyscrasia.Its acronym is derived from its principal characteristics:polyneuropathy,organomegaly,endocrinopathy,M proteins,and skin changes.Here,the authors reported a case of POEMS syndrome that was also associated with Castleman disease.A 53-year-old female patient was admitted to our hospital with limb weakness,numbness,edema,abdominal distention,and fever.Physical examination revealed tetraplegia,paraesthesia,and hyporeflexia in all four limbs,in addition to lymphadenectasis,splenomegaly,skin hyperpigmentation,hypertrichosis,and pitting edema.Laboratory tests and imaging revealed thrombocytosis,hypothyroidism,diabetes,hydropericardium,hydrothorax,splenomegaly,and lymphadenectasis.Electromyography showed the characteristic patterns of both demyelinating disease and axonal degeneration.Serum protein electrophoresis revealed monoclonal immunoglobulin G-lambda paraproteins.Histological examination clearly diagnosed the disease as the hyaline vascular subtype.The final diagnosis in this case was POEMS syndrome in association with Castleman disease.
