Objective:To study the effects of potassium channel tetramerization domain-containing 12 (KCTD-12) overexpression on in vitro proliferation and invasion of gastrointestinal stromal tumor cells.Methods:Gastrointestinal...Objective:To study the effects of potassium channel tetramerization domain-containing 12 (KCTD-12) overexpression on in vitro proliferation and invasion of gastrointestinal stromal tumor cells.Methods:Gastrointestinal stromal tumor cell lines were cultured and randomly divided into KCTD-12 overexpression group and NC overexpression group that were transfected with KCTD-12 overexpression plasmid and negative control plasmid respectively. 24 h after transfection, the expression of proliferation genes, apoptosis genes, PI3K and p38MAPK signaling pathway molecules as well as invasion genes in cells were detected. Results: Ki-67, SCIN, Bcl-2, p-PI3K, p-AKT, p-p38MAPK, CD146, PAR-2, SDF-1 and CXCR4 expression in KCTD-12 overexpression group were significantly lower than those in NC overexpression group while Bax, Caspase-3, Caspase-7, Caspase-9 and p-FOXO1 expression were significantly higher than those in NC overexpression group.Conclusion:KCTD-12 can inhibit the in vitro proliferation and invasion of gastrointestinal stromal tumor cells.展开更多
文摘Objective:To study the effects of potassium channel tetramerization domain-containing 12 (KCTD-12) overexpression on in vitro proliferation and invasion of gastrointestinal stromal tumor cells.Methods:Gastrointestinal stromal tumor cell lines were cultured and randomly divided into KCTD-12 overexpression group and NC overexpression group that were transfected with KCTD-12 overexpression plasmid and negative control plasmid respectively. 24 h after transfection, the expression of proliferation genes, apoptosis genes, PI3K and p38MAPK signaling pathway molecules as well as invasion genes in cells were detected. Results: Ki-67, SCIN, Bcl-2, p-PI3K, p-AKT, p-p38MAPK, CD146, PAR-2, SDF-1 and CXCR4 expression in KCTD-12 overexpression group were significantly lower than those in NC overexpression group while Bax, Caspase-3, Caspase-7, Caspase-9 and p-FOXO1 expression were significantly higher than those in NC overexpression group.Conclusion:KCTD-12 can inhibit the in vitro proliferation and invasion of gastrointestinal stromal tumor cells.
