Objective:To study the relationship of the expressions of p53 and mdm2 in leukoplakia cancer.Methods:RT-PCR was used to detect the mRNA of p53,mdm2 in patients with leukoplakia cancer.The frequencies of p53,mdm2 in pe...Objective:To study the relationship of the expressions of p53 and mdm2 in leukoplakia cancer.Methods:RT-PCR was used to detect the mRNA of p53,mdm2 in patients with leukoplakia cancer.The frequencies of p53,mdm2 in peripheral blood were detected by flow cytometric analysis.Results:The expression of p53mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 7.7%,27.3%,33.3%,56.8%,respectively.The frequencies of p53 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.3±0.1)%,(l.6±0.9)%,(1.9±1.1)%,(3.4±1.8)%.The expression of mdm2 mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 0.0%,6.8%,11.1%,37.8%,respectively.The frequencies of mdm2 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.1±0.1)%,(0.8±0.6)%,(1.2±0.8)%,(1.2±0.8)%.There was a positively correlation between p53 mRNA and mdm2 mRNA.Conclusions:The positive rate of p53 and mdm2 cells in the peripheral blood increases in patients with leukoplakia cancer tissue and has positive correlation with the severity of leukoplakia cancer.展开更多
AIM:To investigate T helper 17/regulatory T cell alterations in early severe hepatitis B and the effect of glucocorticoids.METHODS:The study included 20 patients in the early stage of severe hepatitis B(SHB)and 11 hea...AIM:To investigate T helper 17/regulatory T cell alterations in early severe hepatitis B and the effect of glucocorticoids.METHODS:The study included 20 patients in the early stage of severe hepatitis B(SHB)and 11 healthy controls.All patients had elevated T helper 17(Th17)levels,decreased regulatory T(Treg)cell levels,and significant Th17/Treg ratios.RESULTS:After glucocorticoid treatment,16 patients showed improvement with significant decreases in Th17 levels,increases in Treg,and rebalanced Th17/Treg ratios.The four patients who showed no improvement had increases in both Th17 and Treg levels andan even higher Th17/Treg ratio than before.CONCLUSION:Glucocorticoid treatment can rectify Th17/Treg dysregulation in patients with SHB.展开更多
基金supported by Education Department of Anhui(NoKJ2012Z148)
文摘Objective:To study the relationship of the expressions of p53 and mdm2 in leukoplakia cancer.Methods:RT-PCR was used to detect the mRNA of p53,mdm2 in patients with leukoplakia cancer.The frequencies of p53,mdm2 in peripheral blood were detected by flow cytometric analysis.Results:The expression of p53mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 7.7%,27.3%,33.3%,56.8%,respectively.The frequencies of p53 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.3±0.1)%,(l.6±0.9)%,(1.9±1.1)%,(3.4±1.8)%.The expression of mdm2 mRNA in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were 0.0%,6.8%,11.1%,37.8%,respectively.The frequencies of mdm2 in normal oral mucosa,simple oral leukoplakia,no-simple oral leukoplakia and leukoplakia cancer were(0.1±0.1)%,(0.8±0.6)%,(1.2±0.8)%,(1.2±0.8)%.There was a positively correlation between p53 mRNA and mdm2 mRNA.Conclusions:The positive rate of p53 and mdm2 cells in the peripheral blood increases in patients with leukoplakia cancer tissue and has positive correlation with the severity of leukoplakia cancer.
基金Supported by 12 5-year Plan National Science and Technology Key Project of China,No.2012ZX10002-004
文摘AIM:To investigate T helper 17/regulatory T cell alterations in early severe hepatitis B and the effect of glucocorticoids.METHODS:The study included 20 patients in the early stage of severe hepatitis B(SHB)and 11 healthy controls.All patients had elevated T helper 17(Th17)levels,decreased regulatory T(Treg)cell levels,and significant Th17/Treg ratios.RESULTS:After glucocorticoid treatment,16 patients showed improvement with significant decreases in Th17 levels,increases in Treg,and rebalanced Th17/Treg ratios.The four patients who showed no improvement had increases in both Th17 and Treg levels andan even higher Th17/Treg ratio than before.CONCLUSION:Glucocorticoid treatment can rectify Th17/Treg dysregulation in patients with SHB.
