Polypropylene(PP)scaffolds are the most commonly used biomedical scaffolds despite their disadvan-tages,which include problems with adhesion,infection,and inflammatory responses.Here,we report on the successful develo...Polypropylene(PP)scaffolds are the most commonly used biomedical scaffolds despite their disadvan-tages,which include problems with adhesion,infection,and inflammatory responses.Here,we report on the successful development of a facile one-step method to fabricate a series of novel triclosan poly-dopamine polypropylene(TPP)composite scaffolds and thereby effectively improve the biocompatibility and long-term antibacterial properties of PP scaffolds.The antibacterial triclosan can effectively interact with dopamine during biocompatible polydopamine formation on the PP scaffold by one-step green fab-rication.Thanks to the sustained release of triclosan from the biocompatible polydopamine coating,a 5mm×5mm sample of TPP-coated scaffold made with a triclosan concentration of 8 mg-mL^(-1)(referredto herein as TPP-8)exhibited a continuous antibacterial effect against both Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)for more than 15d,at maximum antibacterial volumes of 2 and 5mL,respectively.Our study establishes a new direction for facile long-term antibacterial studies for medical applications.展开更多
OBJECTIVE:To explore the pathologic characteristics of hyperplasia of the mammary gland(HMG) by observing differences in infrared radiation temperature of points of HMG in patients with different syndromes compared wi...OBJECTIVE:To explore the pathologic characteristics of hyperplasia of the mammary gland(HMG) by observing differences in infrared radiation temperature of points of HMG in patients with different syndromes compared with healthy controls.METHODS:AFLIRSystems Therma CAM P30 infrared thermal camera was used to detect the infrared temperature of Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV 12),Qihai(CV 6),Guanyuan(CV 4),Taixi(KI 3),and Taichong(LR 3) in 113 patients with HMG.Of these patients,71 were placed in the Liver Qi stagnation group,34 were placed in the Dysfunction of conception and thoroughfare vessels group,and 8 were placed in the Phlegm and blood stasis in combination group.The infrared radiation temperature of each point in the patients was compared with that of healthy controls,and the differences in the infrared radiation temperatures of the points in the patients were analyzed.RESULTS:Overall,the bilateral corresponding point in both the controls and patients exhibited no significant difference in infrared radiation temperature.In all cases,the infrared radiation temperature of the points from proximal to distal tended to decrease.In a comparison of the patients and controls,the infrared radiation temperature of the trunk points Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV12),Qihai(CV6),and Guanyuan(CV 4) of the patients was higher than that of the controls,while the infrared radiation temperature of the lower extremity points Taixi(KI 3) and Taichong(LR 3) was lower than that of the controls.Of these points,Shanzhong(CV 17)(P=0.0368),Zhongwan(CV 12)(P=0.0028),Qihai(CV 6)(P=0.0085),and Guanyuan(CV4)(P=0.0018) showed significant differences.In a comparison of the corresponding point on the same side in the Liver Qi stagnation group and controls,the infrared radiation temperature of Shanzhong(CV17)(P=0.0089),right-side Qimen(LR 14)(P=0.0382),Zhongwan(CV 12)(P= 0.0000),Qihai(CV 6)(P=0.0011),and Guanyuan(CV 4)(P=0.0000) of the patients was significantly higher than that of the controls,while the differences in the infrared radiation temperature of the other points were not statistically significant(P= 0.0833-0.8397).In a comparison of the corresponding point on the same side in the Dysfunction of conception and thoroughfare vessels group and controls,the infrared radiation temperature of left-side Taichong(LR 3)(P=0.0048),right-side Taichong(LR 3)(P=0.0329),left-side Taixi(KI 3)(P= 0.0171),and right-side Taixi(KI 3)(t=0.544,P= 0.0165) of the patients was significantly lower than that of the controls,while the differences in the infrared radiation temperature of the other points were not statistically significant(P=0.3793-0.9197).In a comparison of the corresponding point on the same side in the Phlegm and blood stasis in combination group and controls,the infrared radiation temperature of Shanzhong(CV 17),Qimen(LR 14),Qihai(CV 6),Guanyuan(CV 4),Taixi(KI 3),Taichong(LR 3),and Zhongwan(CV 12) tended to increase,but without statistical significance(P=0.175-.759).CONCLUSION:The corresponding points of HMG patients with different syndromes are in different deficiency/excess states.Changes in the infrared radiation temperature of the trunk points Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV 12),Qihai(CV 6),and Guanyuan(CV 4) are closely related to the pathological characteristics of the Liver Qi stagnation syndrome of HMG patients,while changes in the infrared radiation temperature of the lower extremity points Taixi(KI 3) and Taichong(LR 3) are closely related to the pathological characteristics of the Dysfunction of conception and thoroughfare vessels syndrome of HMG patients.On the whole,HMG patients with Liver Qi stagnation syndrome are characterized by "upper excess," and those with Dysfunction of conception and thoroughfare vessels syndrome are characterized by"lowerdeficiency."展开更多
The clinical translation of stem cells and their extracellular vesicles(EVs)-based therapy for central nervous system(CNS) diseases is booming. Nevertheless, the insufficient CNS delivery and retention together with t...The clinical translation of stem cells and their extracellular vesicles(EVs)-based therapy for central nervous system(CNS) diseases is booming. Nevertheless, the insufficient CNS delivery and retention together with the invasiveness of current administration routes prevent stem cells or EVs from fully exerting their clinical therapeutic potential. Intranasal(IN) delivery is a possible strategy to solve problems as IN route could circumvent the brain-blood barrier non-invasively and fit repeated dosage regimens. Herein, we gave an overview of studies and clinical trials involved with IN route and discussed the possibility of employing IN delivery to solve problems in stem cells or EVs-based therapy. We reviewed relevant researches that combining stem cells or EVs-based therapy with IN administration and analyzed benefits brought by IN route. Finally, we proposed possible suggestions to facilitate the development of IN delivery of stem cells or EVs.展开更多
When structured illumination is used in digital holographic microscopy(DHM),each direction of the illumination fringe is required to be shifted at least three times to perform the phase-shifting reconstruction.In this...When structured illumination is used in digital holographic microscopy(DHM),each direction of the illumination fringe is required to be shifted at least three times to perform the phase-shifting reconstruction.In this paper,we propose a scheme for spatial resolution enhancement of DHM by using the structured illumination but without phase shifting.The structured illuminations of different directions,which are generated by a spatial light modulator,illuminate the sample sequentially in the object plane.The formed object waves interfere with a reference wave in an off-axis configuration,and a CCD camera records the generated hologram.After the object waves are reconstructed numerically,a synthetic aperture is performed by an iterative algorithm to enhance the spatial resolution.The resolution improvement of the proposed method is proved and demonstrated by both simulation and experiment.展开更多
基金support from the project of the Science and Technology Program of Guangzhou(201704020059 and 201803010074).
文摘Polypropylene(PP)scaffolds are the most commonly used biomedical scaffolds despite their disadvan-tages,which include problems with adhesion,infection,and inflammatory responses.Here,we report on the successful development of a facile one-step method to fabricate a series of novel triclosan poly-dopamine polypropylene(TPP)composite scaffolds and thereby effectively improve the biocompatibility and long-term antibacterial properties of PP scaffolds.The antibacterial triclosan can effectively interact with dopamine during biocompatible polydopamine formation on the PP scaffold by one-step green fab-rication.Thanks to the sustained release of triclosan from the biocompatible polydopamine coating,a 5mm×5mm sample of TPP-coated scaffold made with a triclosan concentration of 8 mg-mL^(-1)(referredto herein as TPP-8)exhibited a continuous antibacterial effect against both Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)for more than 15d,at maximum antibacterial volumes of 2 and 5mL,respectively.Our study establishes a new direction for facile long-term antibacterial studies for medical applications.
基金Supported by the National Basic Research Program of China(2009CB522901)the Key Program of the State Administration of Traditional Chinese Medicine of the People's Republic of Chinathe Shanghai Municipal Science Foundation(11DZ1973300,11ZR1436700)
文摘OBJECTIVE:To explore the pathologic characteristics of hyperplasia of the mammary gland(HMG) by observing differences in infrared radiation temperature of points of HMG in patients with different syndromes compared with healthy controls.METHODS:AFLIRSystems Therma CAM P30 infrared thermal camera was used to detect the infrared temperature of Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV 12),Qihai(CV 6),Guanyuan(CV 4),Taixi(KI 3),and Taichong(LR 3) in 113 patients with HMG.Of these patients,71 were placed in the Liver Qi stagnation group,34 were placed in the Dysfunction of conception and thoroughfare vessels group,and 8 were placed in the Phlegm and blood stasis in combination group.The infrared radiation temperature of each point in the patients was compared with that of healthy controls,and the differences in the infrared radiation temperatures of the points in the patients were analyzed.RESULTS:Overall,the bilateral corresponding point in both the controls and patients exhibited no significant difference in infrared radiation temperature.In all cases,the infrared radiation temperature of the points from proximal to distal tended to decrease.In a comparison of the patients and controls,the infrared radiation temperature of the trunk points Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV12),Qihai(CV6),and Guanyuan(CV 4) of the patients was higher than that of the controls,while the infrared radiation temperature of the lower extremity points Taixi(KI 3) and Taichong(LR 3) was lower than that of the controls.Of these points,Shanzhong(CV 17)(P=0.0368),Zhongwan(CV 12)(P=0.0028),Qihai(CV 6)(P=0.0085),and Guanyuan(CV4)(P=0.0018) showed significant differences.In a comparison of the corresponding point on the same side in the Liver Qi stagnation group and controls,the infrared radiation temperature of Shanzhong(CV17)(P=0.0089),right-side Qimen(LR 14)(P=0.0382),Zhongwan(CV 12)(P= 0.0000),Qihai(CV 6)(P=0.0011),and Guanyuan(CV 4)(P=0.0000) of the patients was significantly higher than that of the controls,while the differences in the infrared radiation temperature of the other points were not statistically significant(P= 0.0833-0.8397).In a comparison of the corresponding point on the same side in the Dysfunction of conception and thoroughfare vessels group and controls,the infrared radiation temperature of left-side Taichong(LR 3)(P=0.0048),right-side Taichong(LR 3)(P=0.0329),left-side Taixi(KI 3)(P= 0.0171),and right-side Taixi(KI 3)(t=0.544,P= 0.0165) of the patients was significantly lower than that of the controls,while the differences in the infrared radiation temperature of the other points were not statistically significant(P=0.3793-0.9197).In a comparison of the corresponding point on the same side in the Phlegm and blood stasis in combination group and controls,the infrared radiation temperature of Shanzhong(CV 17),Qimen(LR 14),Qihai(CV 6),Guanyuan(CV 4),Taixi(KI 3),Taichong(LR 3),and Zhongwan(CV 12) tended to increase,but without statistical significance(P=0.175-.759).CONCLUSION:The corresponding points of HMG patients with different syndromes are in different deficiency/excess states.Changes in the infrared radiation temperature of the trunk points Shanzhong(CV 17),Qimen(LR 14),Zhongwan(CV 12),Qihai(CV 6),and Guanyuan(CV 4) are closely related to the pathological characteristics of the Liver Qi stagnation syndrome of HMG patients,while changes in the infrared radiation temperature of the lower extremity points Taixi(KI 3) and Taichong(LR 3) are closely related to the pathological characteristics of the Dysfunction of conception and thoroughfare vessels syndrome of HMG patients.On the whole,HMG patients with Liver Qi stagnation syndrome are characterized by "upper excess," and those with Dysfunction of conception and thoroughfare vessels syndrome are characterized by"lowerdeficiency."
基金supported by National Natural Science Foundation of China (81973252,82003668)China Postdoctoral Science Foundation (2020M671771)+2 种基金Ten-thousand Talents Program of Zhejiang Province (2018R52049,China)Natural Science Foundation of Zhejiang Province (LQ21H300002,China)Fundamental Research Funds for the Central Universities (2021QNA7021,China)。
文摘The clinical translation of stem cells and their extracellular vesicles(EVs)-based therapy for central nervous system(CNS) diseases is booming. Nevertheless, the insufficient CNS delivery and retention together with the invasiveness of current administration routes prevent stem cells or EVs from fully exerting their clinical therapeutic potential. Intranasal(IN) delivery is a possible strategy to solve problems as IN route could circumvent the brain-blood barrier non-invasively and fit repeated dosage regimens. Herein, we gave an overview of studies and clinical trials involved with IN route and discussed the possibility of employing IN delivery to solve problems in stem cells or EVs-based therapy. We reviewed relevant researches that combining stem cells or EVs-based therapy with IN administration and analyzed benefits brought by IN route. Finally, we proposed possible suggestions to facilitate the development of IN delivery of stem cells or EVs.
基金This work is supported by the National Basic Research Program(973 Program)of China under Grant No.2012CB921900the National Natural Science Foundation of China(NSFC)under Grant Nos.61377008,61107003,and 61275191.
文摘When structured illumination is used in digital holographic microscopy(DHM),each direction of the illumination fringe is required to be shifted at least three times to perform the phase-shifting reconstruction.In this paper,we propose a scheme for spatial resolution enhancement of DHM by using the structured illumination but without phase shifting.The structured illuminations of different directions,which are generated by a spatial light modulator,illuminate the sample sequentially in the object plane.The formed object waves interfere with a reference wave in an off-axis configuration,and a CCD camera records the generated hologram.After the object waves are reconstructed numerically,a synthetic aperture is performed by an iterative algorithm to enhance the spatial resolution.The resolution improvement of the proposed method is proved and demonstrated by both simulation and experiment.