Low sample volume origami-paper-based graphene-modified aptasensors for label-free electrochemical detection of cancer biomarker-EGFR

In this work,an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor(EGFR)by employing anti-EGFR aptamers as the bio-recognition element.The device used the concept of paper-folding,or origami,to serve as a valve between sample introduction and detection,so reducing sampling volumes and improving operation convenience.Amino-functionalized graphene(NH 2-GO)/thionine(THI)/gold particle(AuNP)nanocomposites were used to modify the working electrode not only to generate the electrochemical signals,but also to provide an environment conducive to aptamer immobilization.Electrochemical characterization revealed that the formation of an insulating aptamer–antigen immunocomplex would hinder electron transfer from the sample medium to the working electrode,thus resulting in a lower signal.The experimental results showed that the proposed aptasensor exhibited a linear range from 0.05 to 200 ngmL^(−1)(R^(2)=0.989)and a detection limit of 5pgmL^(−1) for EGFR.The analytical reliability of the proposed paper-based aptasensor was further investigated by analyzing serum samples,showing good agreement with the gold-standard enzyme-linked immunosorbent assay.

Branched hybridization chain reaction-using highly dimensional DNA nanostructures for label-free,reagent-less,multiplexed molecular diagnostics

The specific and multiplexed detection of DNA underpins many analytical methods,including the detection of microorganisms that are important in the medical,veterinary,and environmental sciences.To achieve such measurements generally requires enzyme-mediated amplification of the low concentrations of the target nucleic acid sequences present,together with the precise control of temperature,as well as the use of enzyme-compatible reagents.This inevitably leads to compromises between analytical performance and the complexity of the assay.The hybridization chain reaction(HCR)provides an attractive alternative,as a route to enzyme-free DNA amplification.To date,the linear nucleic acid products,produced during amplification,have not enabled the development of efficient multiplexing strategies,nor the use of label-free analysis.Here,we show that by designing new DNA nanoconstructs,we are able,for the first time,to increase the molecular dimensionality of HCR products,creating highly branched amplification products,which can be readily detected on label-free sensors.To show that this new,branching HCR system offers a route for enzyme-free,label-free DNA detection,we demonstrate the multiplexed detection of a target sequence(as the initiator)in whole blood.In the future,this technology will enable rapid point-of-care multiplexed clinical analysis or in-the-field environmental monitoring.