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Recombinogenic Effect of the Ternary Complex of Copper(II) with Doxycycline and 1,10-Phenanthroline on Somatic Cells of Drosophila melanogaster
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作者 Jeyson cesary Lopes Luisa Mariana Melo Guimaraes +2 位作者 julio cesar nepomuceno Sandra Morelli Robson Jose de Oliveira Junior 《Journal of Pharmacy and Pharmacology》 2018年第5期531-540,共10页
In the search for new anti-tumor agents, exploiting features such as the flexibility of coordination modes of metals have become an alternative strategy for synthesizing pharmaceuticals. It has been shown that the Cu... In the search for new anti-tumor agents, exploiting features such as the flexibility of coordination modes of metals have become an alternative strategy for synthesizing pharmaceuticals. It has been shown that the CuDP (copper(II), doxycycline, and 1,10-phenanthroline) complex cleaves DNA strands by an oxidative mechanism and by intercalating the major groove, resulting in a cytotoxic action. The objective of this study was to assess the mutageinc/recombinogenic effects of the CuDP complex in vivo using the SMART (Somatic Mutation and Recombination Test) in Drosophila melanogaster. Treatments were carried out with third instar larvae at the standard cross and high bioactivation cross using three concentrations of CuDP (6.92, 13.84 or 27.67 mM). The mutagenic doxorubicin (0.4 mM) was used as a positive control and reverse osmosis water as a negative control. For each compound, marked trans-heterozygous and balanced heterozygous individuals were analyzed to determine the mutational and recombinogenic events occurring in the cells, We found that CuDP significantly increased the frequencies of mutant cells in both standard and high bioactivation crosses, mostly by induction of recombination. These data show that CuDP is a direct recombinogenic agent that is independent of bioactivation, 展开更多
关键词 COPPER recombination MUTATION Drosophila melanogaster.
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Effects of a Carbonated Soft Drink on Epitheial Tumor Incidence in Drosophila melanogaster
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作者 Paula Marynella Alves Pereira Lima Priscila Capelari Orsoli +2 位作者 Thaise Gongalves Araujo Douglas Brandao Cardoso julio cesar nepomuceno 《Journal of Pharmacy and Pharmacology》 2018年第3期240-247,共8页
Diet represents an important risk factor for tumor development and numerous food dyes have been tested for their carcinogenic potential. Caramel IV colorant and its byproduct, the 4-methylimidazole (4-MI), are part ... Diet represents an important risk factor for tumor development and numerous food dyes have been tested for their carcinogenic potential. Caramel IV colorant and its byproduct, the 4-methylimidazole (4-MI), are part of the composition of cola soft drinks, although in undefined concentration. Considering that this concentration depends on the manufacturing place, we aimed to evaluate the potential carcinogenic activity of cola soft drinks from Brazil, Canada, Egypt and Netherlands, through the test for detection of epithelial tumor clones (warts) in somatic cells of Drosophila melanogaster. We used 72-hour larvae resulting from the crossing of wts/TM3, Sb^1 virgin females and mwh/mwh males. Larvae were exposed to the media containing the cola soft drinks for 48 hours and the analysis was performed in adults after transformation. Cola soft drinks from Brazil significantly induced epithelial tumor in D. melanogaster, while those from Canada, Egypt and Netherlands did not. In fact, the concentration of 4-MI is higher in cola soft drink commercialized in Brazil. Our results highlight the importance of investigating the toxic potential of current drinks, which may correlate with the rising incidence of malignant diseases. 展开更多
关键词 Carcinogenic effect Drosophila melanogaster caramel IV WARTS
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