目的:探讨^(68)Ga标记的成纤维细胞激活蛋白抑制剂(fibroblast activation protein inhibitor,FAPI)-04正电子发射断层显像/X线计算机体层成像(positron emission tomography and computed tomography,PET/CT)对初诊胰腺癌的诊断价值及...目的:探讨^(68)Ga标记的成纤维细胞激活蛋白抑制剂(fibroblast activation protein inhibitor,FAPI)-04正电子发射断层显像/X线计算机体层成像(positron emission tomography and computed tomography,PET/CT)对初诊胰腺癌的诊断价值及对肿瘤分期的影响。方法:回顾性收集2021年8月至2023年8月于重庆大学附属肿瘤医院就诊的134例因怀疑胰腺占位的患者临床及影像资料。分析^(68)Ga-FAPI-04 PET/CT对胰腺癌原发灶及转移灶的诊断价值;并与传统CT比较,分析^(68)Ga-FAPI-04 PET/CT对胰腺癌肿瘤分期的影响。结果:共计纳入134例患者,其中胰腺癌127例,良性病变7例。^(68)Ga-FAPI-04 PET/CT对胰腺癌原发灶、区域淋巴结及远处转移灶检出率分别为100%(127/127)、68.63%(35/51),95.35%(41/43)。胰腺癌中位最大标准化摄取值(standard uptake value maxium,SUVmax)为14.92,高于胰腺良性病变中位SUVmax(6.1)(Z=−2.921,P=0.003)。与传统CT比较,^(68)Ga-FAPI PET/CT检查后共有32.28%(41/127)胰腺癌患者TNM分期发生改变。3.94%(5/127)患者改变了治疗方案。结论:^(68)Ga-FAPI-04 PET/CT对胰腺癌原发灶诊断具有较高的敏感性,较传统CT可发现更多远处转移病灶,改善患者M分期,有助于全面评估全身肿瘤负荷、制定治疗计划。展开更多
The structure of light diquarks plays a crucial role in formation of exotic hadrons beyond the conventional quark model, especially with regard to the line shapes of bottomed hadron decays. We study the two-body hadro...The structure of light diquarks plays a crucial role in formation of exotic hadrons beyond the conventional quark model, especially with regard to the line shapes of bottomed hadron decays. We study the two-body hadronic weak decays of bottomed baryons and bottomed mesons to probe the light diquark structure and to pin down the quark–quark correlations in the diquark picture. It is found that the light diquark does not favor a compact structure. For instance, the isoscalar diquark [ud] in Λ_(b)^(0) can be easily split and rearranged to form ■via the color-suppressed transition. This provides a hint that the hidden charm pentaquark states produced in Λ_(b)^(0) decays could be the ■chadronic molecular candidates. This quantitative study resolves the apparent conflicts between the production mechanism and the molecular nature of these P_(c) states observed in experiment.展开更多
Transient receptor potential melastatin 2(TRPM2) is an important ion channel that represents a potential target for treating injury caused by cerebral ischemia. However, it is unclear whether reducing TRPM2 expression...Transient receptor potential melastatin 2(TRPM2) is an important ion channel that represents a potential target for treating injury caused by cerebral ischemia. However, it is unclear whether reducing TRPM2 expression can help repair cerebral injury, and if so what the mechanism underlying this process involves. This study investigated the protective effect of reducing TRPM2 expression on pheochromocytoma(PC12) cells injured by oxygen-glucose deprivation(OGD). PC12 cells were transfected with plasmid encoding TRPM2 shRNAS, then subjected to OGD by incubation in glucose-free medium under hypoxic conditions for 8 hours, after which the cells were allowed to reoxygenate for 24 hours. Apoptotic cells, mitochondrial membrane potentials, reactive oxygen species levels, and cellular calcium levels were detected using flow cytometry. The relative expression of C-X-C motif chemokine ligand 2(CXCL2), NACHT, LRR, and PYD domain–containing protein 3(NALP3), and caspase-1 were detected using fluorescence-based quantitative reverse transcription-polymerase chain reaction and western blotting. The rates of apoptosis, mitochondrial membrane potentials, reactive oxygen species levels, and cellular calcium levels in the TRPM2-shRNA + OGD group were lower than those observed in the OGD group. Taken together, these results suggest that TRPM2 knockdown reduces OGD-induced neuronal injury, potentially by inhibiting apoptosis and reducing oxidative stress levels, mitochondrial membrane potentials, intracellular calcium concentrations, and NLRP3 inflammasome activation.展开更多
This article provides a review of Blood Oxygen Level Dependent functional magnetic resonance imaging(BOLD fMRI)applications for presurgical mapping in patients with brain tumors who are being considered for lesion res...This article provides a review of Blood Oxygen Level Dependent functional magnetic resonance imaging(BOLD fMRI)applications for presurgical mapping in patients with brain tumors who are being considered for lesion resection.Initially,the physical principle of the BOLD effect is discussed,followed by a general overview of the aims of presurgical planning.Subsequently,a review of sensorimotor,language and visual paradigms that are typically utilized in clinical fMRI is provided,followed by a brief description of studies demonstrating the clinical impact of preoperative BOLD fMRI.After this thorough introduction to presurgical fMRI,a detailed explanation of the phenomenon of neurovascular uncoupling(NVU),a major limitation of fMRI,is provided,followed by a discussion of the different approaches taken for BOLD cerebrovascular reactivity(CVR)mapping,which is an effective method of detecting NVU.We then include one clinical case which demonstrates the value of CVR mapping in clinical preoperative fMRI interpretation.The paper then concludes with a brief review of applications of CVR mapping other than for presurgical mapping.展开更多
An efficient and selective method for the deprotection of primary alcoholic tert-butyl-dimethylsilyl (TBS) ethers using SnCl_2·2H_2O as catalyst is described. The reaction conditionsallow primary alcoholic TBS et...An efficient and selective method for the deprotection of primary alcoholic tert-butyl-dimethylsilyl (TBS) ethers using SnCl_2·2H_2O as catalyst is described. The reaction conditionsallow primary alcoholic TBS ethers to be desilylated chemoselectively in the presence of phenolicTBS ethers, secondary and tertiary alcolholic TBS ethers, and the extensively used TBDPS-,acetyl-, benzyloxycarbonyl-, p-toluenesulfonyl- and benzyl protective groups.展开更多
This paper starts with brief introduction to the open topic of the CFD and wing tunnel correlation study, followed by a description of the Chinese Aeronautical Establishment（CAE） –Aerodynamic Validation Model（AVM...This paper starts with brief introduction to the open topic of the CFD and wing tunnel correlation study, followed by a description of the Chinese Aeronautical Establishment（CAE） –Aerodynamic Validation Model（AVM） and its wind tunnel test in the German-Dutch Wind tunnels（DNW）. The features of the aerodynamic design, the CFD approach, the wind tunnel model fabrication and the experimental techniques are discussed along with the motivation of the CAEDNW workshop on CFD-wind tunnel correlation study. The workshop objective is focused on the interference from the aero-elastic deformation of the wind tunnel model and the model support system to the aerodynamic performance and CFD validations. The four study cases, geometry and mesh preparation of the workshop are introduced. A comprehensive summary of the CFD results from the organizer and the participants is provided. Major observations, both CFD to CFD and CFD to wind tunnel, are identified and summarized. The CFD results of the participants are in good agreement with each other, and with the wind tunnel test data when the wing deformation and a Z-sting system are included in the CFD, indicating the importance of considering such interference at high subsonic Mach number of 0.85.展开更多
The Chinese Aeronautical Establishment（CAE） Aerodynamic Validation Model（AVM）is a dual-purpose test geometry dedicated to verify the aerodynamic performance of a conceptual intercontinental jet aircraft and to pro...The Chinese Aeronautical Establishment（CAE） Aerodynamic Validation Model（AVM）is a dual-purpose test geometry dedicated to verify the aerodynamic performance of a conceptual intercontinental jet aircraft and to provide a dataset for CFD software validation. To this end, a scaled model of the AVM was tested in the High-Speed Tunnel（HST） of the German-Dutch Wind-tunnels（DNW） with special test consideration and instrumentation. For complementary analysis of experimental results, specific CAE-AVM geometries are analyzed using a CAE inhouse CFD code. The specific geometries consist of a baseline aircraft, an aircraft with a deformed wing shape, and an aircraft with both a deformed wing shape and a representation of the model support system used in the wind tunnel. Detailed analysis of numerical and experimental results is presented; both the combined and individual attributions of wing deformation and support system interference on wing pressure distributions and longitudinal aerodynamic characteristics are summarized.展开更多
Primary hyperoxaluria （PH） is a rare autosoma recessive disorder of glyoxylate metabolism in which specific hepatic enzyme deficiencies result in overproduction of oxalate. Because oxalate is excreted exclusively by...Primary hyperoxaluria （PH） is a rare autosoma recessive disorder of glyoxylate metabolism in which specific hepatic enzyme deficiencies result in overproduction of oxalate. Because oxalate is excreted exclusively by the kidney, hyperoxaluria leads to calcium oxalate nephrolithiasis, nephrocalcinosis, and renal failure. PH are considered rare with a prevalence of 0.1-0.2 per 106 population.2 PH was misdiagnosed in some cases initially and unfortunately a case may not be detected until the post-transplant period by allograft biopsy.展开更多
While the standard model is the most successful theory to describe all the interactions and constituents of elementary particle physics,it has been constantly scrutinized for over four decades.Weak decays of charm qua...While the standard model is the most successful theory to describe all the interactions and constituents of elementary particle physics,it has been constantly scrutinized for over four decades.Weak decays of charm quarks can be used to measure the coupling strength between quarks in different families and serve as an ideal probe for CP violation.As the lowest charm-strange baryons with three different flavors,Ξ;baryons(composed of csu or csd)have been extensively studied in experiments.In this study,we use state-of-the-art lattice QCD techniques to generate 2+1 clover fermion ensembles with two lattice spacings,a=(0.108,0.080 fm).Then,we present the first ab-initio lattice QCD calculation of the Ξ;→Ξ form factors.Our theoretical results for the Ξc→Ξl;v;decay widths are consistent with and approximately two times more precise than the latest measurements by the ALICE and Belle collaborations.Based on the latest experimental measurements,we independently obtain the quark-mixing matrix element |V;|,which is in good agreement with results from other theoretical approaches.展开更多
基金partly supported by the National Natural Science Foundation of China (Grant Nos. 12375073, 12035007, 12205106, and 12105028)Guangdong Provincial Fund (Grant No. 2019QN01X172)+2 种基金Guangdong Major Project of Basic and Applied Basic Research (Grant No. 2020B0301030008)the NSFC and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) through the funds provided to the Sino-German Collaborative Research Center TRR110 “Symmetries and the Emergence of Structure in QCD” (NSFC Grant No. 12070131001, DFG Project-ID 196253076-TRR 110)supported by the Natural Science Foundation of Jiangsu Province (Grant No. BK20200980)
文摘The structure of light diquarks plays a crucial role in formation of exotic hadrons beyond the conventional quark model, especially with regard to the line shapes of bottomed hadron decays. We study the two-body hadronic weak decays of bottomed baryons and bottomed mesons to probe the light diquark structure and to pin down the quark–quark correlations in the diquark picture. It is found that the light diquark does not favor a compact structure. For instance, the isoscalar diquark [ud] in Λ_(b)^(0) can be easily split and rearranged to form ■via the color-suppressed transition. This provides a hint that the hidden charm pentaquark states produced in Λ_(b)^(0) decays could be the ■chadronic molecular candidates. This quantitative study resolves the apparent conflicts between the production mechanism and the molecular nature of these P_(c) states observed in experiment.
基金supported by the National Natural Science Foundation of China,Nos.81671532,81771625(to XF)the Jiangsu Provincial Key Medical Discipline of China,No.ZDXKA2016013(to XF)+3 种基金the Jiangsu Provincial Medical Youth Talent of China,No.QNRC2016758(to XF)the Jiangsu Province Women and Children Health Research Project of China,No.F201750(to XF)the Public Health Technology Project of Suzhou City of China,No.SYS201765(to XF)a grant from the Department of Pediatrics Clinical Center of Suzhou City of China,No.Szzx201504(to XF)。
文摘Transient receptor potential melastatin 2(TRPM2) is an important ion channel that represents a potential target for treating injury caused by cerebral ischemia. However, it is unclear whether reducing TRPM2 expression can help repair cerebral injury, and if so what the mechanism underlying this process involves. This study investigated the protective effect of reducing TRPM2 expression on pheochromocytoma(PC12) cells injured by oxygen-glucose deprivation(OGD). PC12 cells were transfected with plasmid encoding TRPM2 shRNAS, then subjected to OGD by incubation in glucose-free medium under hypoxic conditions for 8 hours, after which the cells were allowed to reoxygenate for 24 hours. Apoptotic cells, mitochondrial membrane potentials, reactive oxygen species levels, and cellular calcium levels were detected using flow cytometry. The relative expression of C-X-C motif chemokine ligand 2(CXCL2), NACHT, LRR, and PYD domain–containing protein 3(NALP3), and caspase-1 were detected using fluorescence-based quantitative reverse transcription-polymerase chain reaction and western blotting. The rates of apoptosis, mitochondrial membrane potentials, reactive oxygen species levels, and cellular calcium levels in the TRPM2-shRNA + OGD group were lower than those observed in the OGD group. Taken together, these results suggest that TRPM2 knockdown reduces OGD-induced neuronal injury, potentially by inhibiting apoptosis and reducing oxidative stress levels, mitochondrial membrane potentials, intracellular calcium concentrations, and NLRP3 inflammasome activation.
文摘This article provides a review of Blood Oxygen Level Dependent functional magnetic resonance imaging(BOLD fMRI)applications for presurgical mapping in patients with brain tumors who are being considered for lesion resection.Initially,the physical principle of the BOLD effect is discussed,followed by a general overview of the aims of presurgical planning.Subsequently,a review of sensorimotor,language and visual paradigms that are typically utilized in clinical fMRI is provided,followed by a brief description of studies demonstrating the clinical impact of preoperative BOLD fMRI.After this thorough introduction to presurgical fMRI,a detailed explanation of the phenomenon of neurovascular uncoupling(NVU),a major limitation of fMRI,is provided,followed by a discussion of the different approaches taken for BOLD cerebrovascular reactivity(CVR)mapping,which is an effective method of detecting NVU.We then include one clinical case which demonstrates the value of CVR mapping in clinical preoperative fMRI interpretation.The paper then concludes with a brief review of applications of CVR mapping other than for presurgical mapping.
文摘An efficient and selective method for the deprotection of primary alcoholic tert-butyl-dimethylsilyl (TBS) ethers using SnCl_2·2H_2O as catalyst is described. The reaction conditionsallow primary alcoholic TBS ethers to be desilylated chemoselectively in the presence of phenolicTBS ethers, secondary and tertiary alcolholic TBS ethers, and the extensively used TBDPS-,acetyl-, benzyloxycarbonyl-, p-toluenesulfonyl- and benzyl protective groups.
文摘This paper starts with brief introduction to the open topic of the CFD and wing tunnel correlation study, followed by a description of the Chinese Aeronautical Establishment（CAE） –Aerodynamic Validation Model（AVM） and its wind tunnel test in the German-Dutch Wind tunnels（DNW）. The features of the aerodynamic design, the CFD approach, the wind tunnel model fabrication and the experimental techniques are discussed along with the motivation of the CAEDNW workshop on CFD-wind tunnel correlation study. The workshop objective is focused on the interference from the aero-elastic deformation of the wind tunnel model and the model support system to the aerodynamic performance and CFD validations. The four study cases, geometry and mesh preparation of the workshop are introduced. A comprehensive summary of the CFD results from the organizer and the participants is provided. Major observations, both CFD to CFD and CFD to wind tunnel, are identified and summarized. The CFD results of the participants are in good agreement with each other, and with the wind tunnel test data when the wing deformation and a Z-sting system are included in the CFD, indicating the importance of considering such interference at high subsonic Mach number of 0.85.
文摘The Chinese Aeronautical Establishment（CAE） Aerodynamic Validation Model（AVM）is a dual-purpose test geometry dedicated to verify the aerodynamic performance of a conceptual intercontinental jet aircraft and to provide a dataset for CFD software validation. To this end, a scaled model of the AVM was tested in the High-Speed Tunnel（HST） of the German-Dutch Wind-tunnels（DNW） with special test consideration and instrumentation. For complementary analysis of experimental results, specific CAE-AVM geometries are analyzed using a CAE inhouse CFD code. The specific geometries consist of a baseline aircraft, an aircraft with a deformed wing shape, and an aircraft with both a deformed wing shape and a representation of the model support system used in the wind tunnel. Detailed analysis of numerical and experimental results is presented; both the combined and individual attributions of wing deformation and support system interference on wing pressure distributions and longitudinal aerodynamic characteristics are summarized.
文摘Primary hyperoxaluria （PH） is a rare autosoma recessive disorder of glyoxylate metabolism in which specific hepatic enzyme deficiencies result in overproduction of oxalate. Because oxalate is excreted exclusively by the kidney, hyperoxaluria leads to calcium oxalate nephrolithiasis, nephrocalcinosis, and renal failure. PH are considered rare with a prevalence of 0.1-0.2 per 106 population.2 PH was misdiagnosed in some cases initially and unfortunately a case may not be detected until the post-transplant period by allograft biopsy.
基金Supported in part by Natural Science Foundation of China(11735010,U2032102,11653003,12005130,11521505,12070131001,11975127,11935017,12005130,12125503)Natural Science Foundation of Shanghai(15DZ2272100)+5 种基金the China Postdoctoral Science Foundation and the National Postdoctoral Program for Innovative Talents(BX20190207)National Key Research and Development Program of China(2020YFA0406400)Jiangsu Specially Appointed Professor Programthe Strategic Priority Research Program of Chinese Academy of Sciences(XDB34030300,XDPB15)a NSFC-DFG joint grant(12061131006,SCHA 458/22)Chinese Academy of Science CAS Strategic Priority Research Program of Chinese Academy of Sciences(XDC01040100)。
文摘While the standard model is the most successful theory to describe all the interactions and constituents of elementary particle physics,it has been constantly scrutinized for over four decades.Weak decays of charm quarks can be used to measure the coupling strength between quarks in different families and serve as an ideal probe for CP violation.As the lowest charm-strange baryons with three different flavors,Ξ;baryons(composed of csu or csd)have been extensively studied in experiments.In this study,we use state-of-the-art lattice QCD techniques to generate 2+1 clover fermion ensembles with two lattice spacings,a=(0.108,0.080 fm).Then,we present the first ab-initio lattice QCD calculation of the Ξ;→Ξ form factors.Our theoretical results for the Ξc→Ξl;v;decay widths are consistent with and approximately two times more precise than the latest measurements by the ALICE and Belle collaborations.Based on the latest experimental measurements,we independently obtain the quark-mixing matrix element |V;|,which is in good agreement with results from other theoretical approaches.
