Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery remain.For instance,compound K(CK)is a ...Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery remain.For instance,compound K(CK)is a major metabolite of ginsenosides that is known to protect against joint degeneration by inhibiting the production of inflammatory cytokines and the activation of immune cells.However,its effects on cartilage degradation and tissue regeneration remain unclear.Additionally,tissue-adhesive drug delivery depots that stably adhere to cartilage defects are required for CK delivery.In this study,CK-loaded adhesive patches were reported to seal cartilage defects and deliver CK to defect sites,preventing cartilage degradation and accelerating cartilage tissue regeneration.Adhesive patches are stable and suitable for application in surgical procedures under physiological conditions and show excellent adhesiveness to cartilage surfaces.In addition,there were no significant differences in the adhesive polymeric networks before and after CK loading.CK-loaded hydrocaffeic acid-conjugated chitosan patches significantly inhibited the stimulation of cartilage-degrading enzymes and apoptosis in osteoarthritic cartilage by releasing CK in cartilage defects.Additionally,the NFkB signaling pathway of released CK from the adhesive patches in the treatment of osteoarthritis is revealed.Thus,the CK-loaded adhesive patches are expected to significantly contribute to cartilage regeneration.展开更多
基金supported by a National Research Foundation of Korea(NRF)grant funded by the Korean Government(MSIT)(2022R1A4A1031259 and 2021R1I1A3041149)to E.-J.J.by a Korean Fund for Regenerative Medicine(KFRM)grant funded by the Korean Government(Ministry of Science and ICT,Ministry of Health&Welfare)(22A0103L1)to J.H.R.
文摘Biomaterial-based drug delivery systems have been developed to expedite cartilage regeneration;however,challenges related to drug recovery,validation,and efficient drug delivery remain.For instance,compound K(CK)is a major metabolite of ginsenosides that is known to protect against joint degeneration by inhibiting the production of inflammatory cytokines and the activation of immune cells.However,its effects on cartilage degradation and tissue regeneration remain unclear.Additionally,tissue-adhesive drug delivery depots that stably adhere to cartilage defects are required for CK delivery.In this study,CK-loaded adhesive patches were reported to seal cartilage defects and deliver CK to defect sites,preventing cartilage degradation and accelerating cartilage tissue regeneration.Adhesive patches are stable and suitable for application in surgical procedures under physiological conditions and show excellent adhesiveness to cartilage surfaces.In addition,there were no significant differences in the adhesive polymeric networks before and after CK loading.CK-loaded hydrocaffeic acid-conjugated chitosan patches significantly inhibited the stimulation of cartilage-degrading enzymes and apoptosis in osteoarthritic cartilage by releasing CK in cartilage defects.Additionally,the NFkB signaling pathway of released CK from the adhesive patches in the treatment of osteoarthritis is revealed.Thus,the CK-loaded adhesive patches are expected to significantly contribute to cartilage regeneration.
