Background： Endometriosis is a challenging disease with symptoms such as dysmenorrhea and infertility. However, its etiology is still vague and there is still no effective markers or treatment. This study aimed to pr...Background： Endometriosis is a challenging disease with symptoms such as dysmenorrhea and infertility. However, its etiology is still vague and there is still no effective markers or treatment. This study aimed to profile the circular RNAs （circRNAs） expressed in eutopic endometrium from patients with ovarian endometriosis and explore potential clues to the pathogenesis of endometriosis, providing an evidence for clinical diagnosis and treatment. Methods： A total of 63 clinical samples, including control endometrium 01 = 22） and eutopic endoxnetrium （n = 41）, were collected from Peking Union Medical College Hospital between May 1,2016, and December 31,2016. Of them, four samples in each group were used for circRNA microarray. Then, tbur upregulated circRNAs were screened out for quantitative real-time polymerase chain reaction （qRT-PCR） validation. After that, bioinformatics analysis was pertbrmed to predict miRNAs targeted by validated circRNAs and investigate the circRNA-miRNA-mRNA interactions. Results： Among 88 differentially expressed circRNAs, 11 were upregulated and 77 were downregulated in eutopic endometrium of patients with endometriosis, qRT-PCR validation results for two upregulated circRNAs （circ0004712 and circ_O002198） matched the microarray results. The area under the receiver operating characteristic curve of circ_0002198 for distinguishing ovarian endometriosis was 0.846 （95% confidence interval [C1]： 0.752-0.939; P 〈 0.001 ） while that ofcitv_O004712 was 0.704 （95% CI： 0.571-0.837; P = 0.008）. On the basis of target prediction, we depicted the molecular interactions between the identified circRNAs and their dominant target miRNAs, as well as constructed a circRNA-miRNA-mRNA network. Conclusions： This study provides evidence that circRNAs are differentially expressed between eutopic and normal endometrium, which suggests that circRNAs are candidate factors in the activation ofendometriosis, circ_0002198 and circ0004712 may be potential novel biomarkers for the diagnosis of ovarian endometriosis.展开更多
Background： Adenomyosis is a gynecological disorder with symptoms most presenting as dysmenorrhea and heavy menstrual bleeding. However, the presence of lower urinary tract symptoms （LUTS） among women with adenomyo...Background： Adenomyosis is a gynecological disorder with symptoms most presenting as dysmenorrhea and heavy menstrual bleeding. However, the presence of lower urinary tract symptoms （LUTS） among women with adenomyosis remains unclear. This study was designed to determine the prevalence of LUTS and factors related to the severity of these symptoms in this population. Methods： From July 2016 to November 2016, a total of 298 untreated symptomatic adenomyosis patients and 280 age-matched controls were enrolled. Demographics, LUTS, pain symptoms, ultrasouographic uterine size, and serum CA125 level were recorded. LUTS were evaluated using the International Prostate Symptom Score （IPSS） questionnaire. Factors related to the severity of LUTS were detected using the logistic regression analysis presented as odds ratio （OR） and 95% confidence interval （CI）. Results： Compared with the control group, patients with adenomyosis had a greater IPSS total （4 [2-8] vs. 2 [0-3], Z = -8.159, P 〈 0.001 ）, IPSS storage （2 [1-4] vs. [[0 2], Z = -7.361 [P 〈 0.001）, and I PSS voiding （2 [0-4] vs. 0 [0-1], Z = -7.[94, P 〈 0.001）. Of the patients with adenomyosis, 30.2% had moderate-to-severe lower urinary tract symptoms （IPSS 〉8）. The most prevalent LUTS were daytime frequency （40.9%）, lbllowed by nocturia （24.8%）, weak stream （24.2%）, and incomplete emptying （23.5%）. In study group, patients with an IPSS total score ≥8 had higher proportion of menorrhagia （51.1% vs. 30.8%, χ2 = 11. 162 P = 0.025） and larger uterine volumes （183.3 [109.8-273.8] cm3 vs. 148.5 [96.4-262.7] cm3, Z=- 1.441, P = 0.150） compared to patients with an IPSS total score 〈8. On multivariate logistic regression analysis, patients with menorrhagia were associated with an increased risk of an IPSS total score 〉8 （OR： 2.309, 95% CI： 1.310-4.070, P= 0.004）, an IPSS storage subscore 〉4 （OR： 2.422, 95% CI： 1.395-4.206, P= 0.002）, and an IPSS voiding subscore 〉5 （OR： 1.971, 95% （CI： 1.176-3.302, p= 0.010）. However, patients with uterine volume more than 180 cm3 had more than 2-fold risk of bearing IPSS total score ≥8 （OR： 2.437, 95% CI： 1.381-4.300, P = 0.002）, 1PSS storage subscore 〉4 （OR： 2.486, 95% CI： 1.433-4.314, P =0.001）, and IPSS voiding subscore ≥5 （OR： 2.700, 95% （CI： 1.485-4.908, P=0.001）. Conclusions： Lower urinary tract synaptoms are prevalent in patients with symptomatic adenomyosis and greatly affect patients＇ quality of life. Menorrhagia and large uterine volume could be potential risk factors that increase the occurrence of moderate-to-severe LUTS.展开更多
Introduction The laparoscopic technique has been used for colorectal surgery for decades.Minimally invasive surgery is executed differently in operations on the right colon,left colon,and the rectum[1].Sometimes,the a...Introduction The laparoscopic technique has been used for colorectal surgery for decades.Minimally invasive surgery is executed differently in operations on the right colon,left colon,and the rectum[1].Sometimes,the abdominal incision is critical for rectal surgery,which can be used for extraction of the resected specimen and for creation of the diverting stoma.However,for right or left colectomy,once the anastomosis is performed intra-corporeally,then the specimen can be extracted through any incision(abdominal wall or vaginal)to enhance post-operative recovery.展开更多
Infinite coordination polymers are recognized as excellent platform for functionalization.Dithienylethene motifs,which are one of the most attractive functional moieties,were incorporated into an infinite coordination...Infinite coordination polymers are recognized as excellent platform for functionalization.Dithienylethene motifs,which are one of the most attractive functional moieties,were incorporated into an infinite coordination polymer,to deliver a‘‘smart’’porous material that can response to external stimuli.The obtained dithienylethene-based infinite coordination polymers（named Cu-DTEDBA）share the advantages of both infinite coordination polymers（porosity and stability）and dithienylethene motifs（photochromism）.The physical and chemical properties of Cu-DTEDBA were characterized by FTIR,TEM,SEM,XRD,TGA,UV–vis,EDX and BET.Moreover,the combination of dithienylethene and infinite coordination polymers gives rise to a synergistic effect,which induces functional behaviors of ammonia sensor applications.Both open and closed forms of Cu-DTEDBA exhibit distinct colorimetric change upon exposure to gaseous ammonia,which is not observed in dithienylethene free molecules.展开更多
基金This study was supported by grants from the National Key R＆D Program of China （No. 2017YFC1001200） and National Natural Science Foundation of China （No. 81270681 ）.
文摘Background： Endometriosis is a challenging disease with symptoms such as dysmenorrhea and infertility. However, its etiology is still vague and there is still no effective markers or treatment. This study aimed to profile the circular RNAs （circRNAs） expressed in eutopic endometrium from patients with ovarian endometriosis and explore potential clues to the pathogenesis of endometriosis, providing an evidence for clinical diagnosis and treatment. Methods： A total of 63 clinical samples, including control endometrium 01 = 22） and eutopic endoxnetrium （n = 41）, were collected from Peking Union Medical College Hospital between May 1,2016, and December 31,2016. Of them, four samples in each group were used for circRNA microarray. Then, tbur upregulated circRNAs were screened out for quantitative real-time polymerase chain reaction （qRT-PCR） validation. After that, bioinformatics analysis was pertbrmed to predict miRNAs targeted by validated circRNAs and investigate the circRNA-miRNA-mRNA interactions. Results： Among 88 differentially expressed circRNAs, 11 were upregulated and 77 were downregulated in eutopic endometrium of patients with endometriosis, qRT-PCR validation results for two upregulated circRNAs （circ0004712 and circ_O002198） matched the microarray results. The area under the receiver operating characteristic curve of circ_0002198 for distinguishing ovarian endometriosis was 0.846 （95% confidence interval [C1]： 0.752-0.939; P 〈 0.001 ） while that ofcitv_O004712 was 0.704 （95% CI： 0.571-0.837; P = 0.008）. On the basis of target prediction, we depicted the molecular interactions between the identified circRNAs and their dominant target miRNAs, as well as constructed a circRNA-miRNA-mRNA network. Conclusions： This study provides evidence that circRNAs are differentially expressed between eutopic and normal endometrium, which suggests that circRNAs are candidate factors in the activation ofendometriosis, circ_0002198 and circ0004712 may be potential novel biomarkers for the diagnosis of ovarian endometriosis.
文摘Background： Adenomyosis is a gynecological disorder with symptoms most presenting as dysmenorrhea and heavy menstrual bleeding. However, the presence of lower urinary tract symptoms （LUTS） among women with adenomyosis remains unclear. This study was designed to determine the prevalence of LUTS and factors related to the severity of these symptoms in this population. Methods： From July 2016 to November 2016, a total of 298 untreated symptomatic adenomyosis patients and 280 age-matched controls were enrolled. Demographics, LUTS, pain symptoms, ultrasouographic uterine size, and serum CA125 level were recorded. LUTS were evaluated using the International Prostate Symptom Score （IPSS） questionnaire. Factors related to the severity of LUTS were detected using the logistic regression analysis presented as odds ratio （OR） and 95% confidence interval （CI）. Results： Compared with the control group, patients with adenomyosis had a greater IPSS total （4 [2-8] vs. 2 [0-3], Z = -8.159, P 〈 0.001 ）, IPSS storage （2 [1-4] vs. [[0 2], Z = -7.361 [P 〈 0.001）, and I PSS voiding （2 [0-4] vs. 0 [0-1], Z = -7.[94, P 〈 0.001）. Of the patients with adenomyosis, 30.2% had moderate-to-severe lower urinary tract symptoms （IPSS 〉8）. The most prevalent LUTS were daytime frequency （40.9%）, lbllowed by nocturia （24.8%）, weak stream （24.2%）, and incomplete emptying （23.5%）. In study group, patients with an IPSS total score ≥8 had higher proportion of menorrhagia （51.1% vs. 30.8%, χ2 = 11. 162 P = 0.025） and larger uterine volumes （183.3 [109.8-273.8] cm3 vs. 148.5 [96.4-262.7] cm3, Z=- 1.441, P = 0.150） compared to patients with an IPSS total score 〈8. On multivariate logistic regression analysis, patients with menorrhagia were associated with an increased risk of an IPSS total score 〉8 （OR： 2.309, 95% CI： 1.310-4.070, P= 0.004）, an IPSS storage subscore 〉4 （OR： 2.422, 95% CI： 1.395-4.206, P= 0.002）, and an IPSS voiding subscore 〉5 （OR： 1.971, 95% （CI： 1.176-3.302, p= 0.010）. However, patients with uterine volume more than 180 cm3 had more than 2-fold risk of bearing IPSS total score ≥8 （OR： 2.437, 95% CI： 1.381-4.300, P = 0.002）, 1PSS storage subscore 〉4 （OR： 2.486, 95% CI： 1.433-4.314, P =0.001）, and IPSS voiding subscore ≥5 （OR： 2.700, 95% （CI： 1.485-4.908, P=0.001）. Conclusions： Lower urinary tract synaptoms are prevalent in patients with symptomatic adenomyosis and greatly affect patients＇ quality of life. Menorrhagia and large uterine volume could be potential risk factors that increase the occurrence of moderate-to-severe LUTS.
文摘Introduction The laparoscopic technique has been used for colorectal surgery for decades.Minimally invasive surgery is executed differently in operations on the right colon,left colon,and the rectum[1].Sometimes,the abdominal incision is critical for rectal surgery,which can be used for extraction of the resected specimen and for creation of the diverting stoma.However,for right or left colectomy,once the anastomosis is performed intra-corporeally,then the specimen can be extracted through any incision(abdominal wall or vaginal)to enhance post-operative recovery.
基金supported by National Basic Research Program of China (No. 2013CB733501)National Natural Science Foundation of China (Nos. 91334203, 21376074, 21402050)Fundamental Research Funds for the Central Universities of China (No. WK1314008)
文摘Infinite coordination polymers are recognized as excellent platform for functionalization.Dithienylethene motifs,which are one of the most attractive functional moieties,were incorporated into an infinite coordination polymer,to deliver a‘‘smart’’porous material that can response to external stimuli.The obtained dithienylethene-based infinite coordination polymers（named Cu-DTEDBA）share the advantages of both infinite coordination polymers（porosity and stability）and dithienylethene motifs（photochromism）.The physical and chemical properties of Cu-DTEDBA were characterized by FTIR,TEM,SEM,XRD,TGA,UV–vis,EDX and BET.Moreover,the combination of dithienylethene and infinite coordination polymers gives rise to a synergistic effect,which induces functional behaviors of ammonia sensor applications.Both open and closed forms of Cu-DTEDBA exhibit distinct colorimetric change upon exposure to gaseous ammonia,which is not observed in dithienylethene free molecules.
