Objectives To develop a high-performance liquid chromatography （HPLC） tandem mass spectrometry method for screening and identifying the multiple constituents in the complex Chinese materia medica （CMM） formulation...Objectives To develop a high-performance liquid chromatography （HPLC） tandem mass spectrometry method for screening and identifying the multiple constituents in the complex Chinese materia medica （CMM） formulation Sini Decoction. Methods The chemical constituents in Sini prescription as an entirety were isolated and analyzed by HPLC coupled with electrospray ionization ion-trap mass spectrometry （ESI-IT-MS） in order to clarify the chemical components of Sini Decoction and explore the chemical methods of studying CMM prescriptions. Results Twenty-five compounds were isolated from the water extract of this decoction. Through chemical and spectral analyses, the structures of 20 compounds were elucidated, including 15 flavonoids, one triterpene, two coumarins, and two other types of compounds. By using HPLC-DAD-MSn technique, the MSn cleavage behaviors of the flavonoids have been summarized as well as the origin of the isolated compounds was assigned. Conclusion The phytochemical compositon of Sini prescription is tentatively characterized by HPLC-DAD-ESI-IT-MS.. The results of the experiments demonstrate that the combination of HPLC and ESI-MSn is a sensitive and effective tool for rapidly investigating the complex CMM formulations.展开更多
To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G （1）, was isolated from the roots of Vernonia cumingiana Benth. （Compositae）. The struc...To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G （1）, was isolated from the roots of Vernonia cumingiana Benth. （Compositae）. The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 （2） and 24-methylenelanost-9（11）- en-3β-ol acetate （3） were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.展开更多
文摘Objectives To develop a high-performance liquid chromatography （HPLC） tandem mass spectrometry method for screening and identifying the multiple constituents in the complex Chinese materia medica （CMM） formulation Sini Decoction. Methods The chemical constituents in Sini prescription as an entirety were isolated and analyzed by HPLC coupled with electrospray ionization ion-trap mass spectrometry （ESI-IT-MS） in order to clarify the chemical components of Sini Decoction and explore the chemical methods of studying CMM prescriptions. Results Twenty-five compounds were isolated from the water extract of this decoction. Through chemical and spectral analyses, the structures of 20 compounds were elucidated, including 15 flavonoids, one triterpene, two coumarins, and two other types of compounds. By using HPLC-DAD-MSn technique, the MSn cleavage behaviors of the flavonoids have been summarized as well as the origin of the isolated compounds was assigned. Conclusion The phytochemical compositon of Sini prescription is tentatively characterized by HPLC-DAD-ESI-IT-MS.. The results of the experiments demonstrate that the combination of HPLC and ESI-MSn is a sensitive and effective tool for rapidly investigating the complex CMM formulations.
文摘To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G （1）, was isolated from the roots of Vernonia cumingiana Benth. （Compositae）. The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 （2） and 24-methylenelanost-9（11）- en-3β-ol acetate （3） were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.
