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Epstein-Barr virus in hepatocellular carcinogenesis 被引量:6
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作者 WeiLi Bao-AnWu +6 位作者 Yong-MingZeng Guang-CanChen Xin-XinLi jun-tianchen Yu-WenGuo Man-HongLi YiZeng 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第23期3409-3413,共5页
AIM: In recent years, studies have suggested that EpsteinBarr virus (EBV) is associated with HCC. The present study was to determine the prevalence of EBV in HCC patients,and whether EBV acted synergistically with hep... AIM: In recent years, studies have suggested that EpsteinBarr virus (EBV) is associated with HCC. The present study was to determine the prevalence of EBV in HCC patients,and whether EBV acted synergistically with hepatitis viruses in HCC carcinogenesis.METHODS: Liver tissue 115 HCC patients and 26 noncarcinoma patients were studied, Polymerase chain reaction (PCR) was performed to detect EBV BarnHI W DNA, EBV LMP1 DNA, HI3V X DNA, and HBV S DNA, Reverse transcription PCR (RT-PCR) was performed to detect HCV RNA and HDV RNA, Immunohistochemistry was performed to detect LMP1,HBsAg, HBcAg and HCV, The positive ratios were compared between HCC group and control group by X^2 test,RESULTS: Totally, 78 HCC samples whose 13-globulin DNA was positively detected by amplified PCR were selected,PCR was performed in all cases for EI3V DNA and HBV DNA,RT-PCR was performed in 18 cases for HCV RNA and HDV RNA. EBV BarnHI W and EBV LMP1 were positive in 18 and 6 cases, respectively. HBV X gene and HBV S gene were positive in 42 and 27 cases respectively. HCV was positive in one of the 18 cases, and none was positive for HDV. The positive rates were 28.2% (22 of 78) for EBV DNA (BarnHI W and/or LMP1) and 56.4% (44 of 78) for HBV DNA (X gene and/or S gene) respectively. In addition, 12 cases were positive for both EBV DNA and HBV DNA. Among the 26 cases in the control group, 2 cases were positive for EBV BarnHI W, 4 positive for HBV X gene and 3 positive for HBV S gene. The positive rates were 8.0% (2 of 26) and 23.1% (6 of 26),respectively, for EBV DNA and HBV DNA. The result of DNA sequencing of BamHI W was 100% homologous with the corresponding sequence of B95-8. There was significant difference in EBV infection rate between HCC patients and controls (X^2 = 4.622, P<0.05). The difference in HBV infection rate was also significant (X^2 = 8.681, P<0.05). However, there was no obvious correlation between HBV and EBV in HCC patients (X^2 = 0.835,/)>0.05). LMP1, HBV (HBsAg, HI3cAg) and HCV were detected positively in 25, 45 and 6 of 78 cases of HCC tissues respectively. In the 26 control cases,the corresponding positive cases were 2, 4 and O. The difference in EBV infection rate between HCC patients and control cases was statistically significant (X^2= 6.02,P<0.05). The difference in HBV infection rate was also statistically significant (X^2 = 10.03, P<0.05). In the 25 cases with positive LMP1 expression, 6 were in the nuclei of tumor cells, 9 in the cytoplasm of tumor cells and 10 in mesenchymal lymphocyte cytoplasm.CONCLUSION: The existence of EBV infection in HCC tissues suggests that EBV may be involved in the hepatocellular carcinogenesis in China. HBV infection may be a major cause of HCC. There is no correlation between EBV and HBV in the development of HCC. The prevalence of HCV infection is low in our area, and HDV appears not to play a direct role in hepatocellular carcinogenesis. 展开更多
关键词 EBV 爱泼斯坦-巴尔病毒 肝细胞 致癌作用 肿瘤 肝癌
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