A cementitious material was prepared by mixing 80wt% Si-Mn slag powder, 10wt% lime, and 10wt% anhydrite. The compressive strength of mortar samples reaches 51.48 MPa after 28 d curing. The analyses of X-ray diffracti...A cementitious material was prepared by mixing 80wt% Si-Mn slag powder, 10wt% lime, and 10wt% anhydrite. The compressive strength of mortar samples reaches 51.48 MPa after 28 d curing. The analyses of X-ray diffraction （XRD） and scanning electron microscopy （SEM） show that much ettringite is formed in the sample cured for 3 d, and C-S-H gel increases rapidly during subsequent curing. Nuclear magnetic resonance （NMR） analysis of 29Si and 27Al and infrared spectroscopy （IR） analysis show that aluminum decomposition from tetrahedral network of the slag glass and its subsequent migration and re-combination play an important role in the process of hydration and strength development of the samples.展开更多
Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine （8-OHdG） in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid （INH） -induced oxidative D...Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine （8-OHdG） in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid （INH） -induced oxidative DNA damage. Methods Precision-cut liver slices （300 μm） were prepared from male rats, and incubated with INH （0.018 mol/L） for 2 h after 1 h preincubation. DNA in the slices was extracted and digested into free nucleosides at 37℃ . The samples were injected into HPLC-MS/MS after the proteins were removed. The level of oxidative DNA damage was estimated using the ratio of 8-OHdG to deoxyguanosine （dG）. Results The limit of detection of 8-OHdG was 1 ng/mL （S/N=3） and the intra-assay relative standard variation was 3.38% when one transition 284.3/168.4 was used as a quantifier and another two transitions 284.3/140.2, 306.1/190.2 as qualifiers. 8-OHdG and dG were well separated, as indicated by elution at 10.02 and 7.37 min, respectively. INH significantly increased the ratio of 8-OHdG to dG in rat liver slices （P〈0.05）. Conclusion 8-OHdG in precision-cut liver slices could be sensitively determined by HPLC-MS/MS. HPLC-MS/MS coupled with precision-cut tissue slices is a fast and reliable analytical technique to evaluate oxidative DNA damage of target tissues caused by procarcinogens and cytotoxins.展开更多
基金supported by the National High Technology Research and Development Program (No.2006BAC21B03)
文摘A cementitious material was prepared by mixing 80wt% Si-Mn slag powder, 10wt% lime, and 10wt% anhydrite. The compressive strength of mortar samples reaches 51.48 MPa after 28 d curing. The analyses of X-ray diffraction （XRD） and scanning electron microscopy （SEM） show that much ettringite is formed in the sample cured for 3 d, and C-S-H gel increases rapidly during subsequent curing. Nuclear magnetic resonance （NMR） analysis of 29Si and 27Al and infrared spectroscopy （IR） analysis show that aluminum decomposition from tetrahedral network of the slag glass and its subsequent migration and re-combination play an important role in the process of hydration and strength development of the samples.
基金This research was supported by the National Natural Science Foundation of China (No. 30600773).
文摘Objective To establish a fast and sensitive method for the detection of 8-hydroxy-2’-deoxyguanosine （8-OHdG） in precision-cut rat liver slices by HPLC-MS/MS and to investigate isoniazid （INH） -induced oxidative DNA damage. Methods Precision-cut liver slices （300 μm） were prepared from male rats, and incubated with INH （0.018 mol/L） for 2 h after 1 h preincubation. DNA in the slices was extracted and digested into free nucleosides at 37℃ . The samples were injected into HPLC-MS/MS after the proteins were removed. The level of oxidative DNA damage was estimated using the ratio of 8-OHdG to deoxyguanosine （dG）. Results The limit of detection of 8-OHdG was 1 ng/mL （S/N=3） and the intra-assay relative standard variation was 3.38% when one transition 284.3/168.4 was used as a quantifier and another two transitions 284.3/140.2, 306.1/190.2 as qualifiers. 8-OHdG and dG were well separated, as indicated by elution at 10.02 and 7.37 min, respectively. INH significantly increased the ratio of 8-OHdG to dG in rat liver slices （P〈0.05）. Conclusion 8-OHdG in precision-cut liver slices could be sensitively determined by HPLC-MS/MS. HPLC-MS/MS coupled with precision-cut tissue slices is a fast and reliable analytical technique to evaluate oxidative DNA damage of target tissues caused by procarcinogens and cytotoxins.
