OBJECTIVE To investigate the anti-irradiation effects of a polypeptide from Chlamys farreri (PCF) on γ-ray induced damage to mouse thymocytes.METHODS Thymocytes were randomly divided into 6 groups including an untrea...OBJECTIVE To investigate the anti-irradiation effects of a polypeptide from Chlamys farreri (PCF) on γ-ray induced damage to mouse thymocytes.METHODS Thymocytes were randomly divided into 6 groups including an untreated control, a model group, groups treated with 5, 2.5 and 1.25mg PCF/ml and a 0.1% vitamin C group. Cell viability, morphology, nucleic acid and enzymatic changes of the cells 3h after 3Gy γ-ray irradiation at a dose rate of 0.6Gy/min were determined.RESULTS γ-Ray irradiation decreased the cell viability and SOD activity and increased MDA levels and the apoptotic number Of cells. PCF increased cell viability and SOD activity and decreased MDA levels and the apoptotic number of the cells in a dose-dependant manner.CONCLUSION PCF pretreatment car)attenuate cytotoxicity, inhibit apoptosi.s,.reduce the level of MDA and maintain the activity of SOD.Therefore PCF has anti-irradiation effects on γ-ray induced damage of mouse thymocytes.展开更多
文摘OBJECTIVE To investigate the anti-irradiation effects of a polypeptide from Chlamys farreri (PCF) on γ-ray induced damage to mouse thymocytes.METHODS Thymocytes were randomly divided into 6 groups including an untreated control, a model group, groups treated with 5, 2.5 and 1.25mg PCF/ml and a 0.1% vitamin C group. Cell viability, morphology, nucleic acid and enzymatic changes of the cells 3h after 3Gy γ-ray irradiation at a dose rate of 0.6Gy/min were determined.RESULTS γ-Ray irradiation decreased the cell viability and SOD activity and increased MDA levels and the apoptotic number Of cells. PCF increased cell viability and SOD activity and decreased MDA levels and the apoptotic number of the cells in a dose-dependant manner.CONCLUSION PCF pretreatment car)attenuate cytotoxicity, inhibit apoptosi.s,.reduce the level of MDA and maintain the activity of SOD.Therefore PCF has anti-irradiation effects on γ-ray induced damage of mouse thymocytes.
