Background:To date,there is no approved blood-based biomarker for breast cancer detection.Herein,we aimed to assess semaphorin 4C(SEMA4C),a pivotal protein involved in breast cancer progression,as a serum diagnostic b...Background:To date,there is no approved blood-based biomarker for breast cancer detection.Herein,we aimed to assess semaphorin 4C(SEMA4C),a pivotal protein involved in breast cancer progression,as a serum diagnostic biomarker.Methods:We included 6,213 consecutive inpatients from Tongji Hospital,Qilu Hospital,and Hubei Cancer Hospital.Training cohort and two validation cohorts were introduced for diagnostic exploration and validation.A pan-cancer cohort was used to independently explore the diagnostic potential of SEMA4C among solid tumors.Breast cancer patients who underwent mass excision prior to modified radical mastectomy were also analyzed.We hypothesized that increased pretreatment serum SEMA4C levels,measured using optimized in-house enzymelinked immunosorbent assay kits,could detect breast cancer.The endpoints were diagnostic performance,including area under the receiver operating characteristic curve(AUC),sensitivity,and specificity.Post-surgery pathological diagnosis was the reference standard and breast cancer staging followed the TNM classification.There was no restriction on disease stage for eligibilities.Results:We included 2667 inpatients with breast lesions,2378 patients with other solid tumors,and 1168 healthy participants.Specifically,118 patients with breast cancer were diagnosed with stage 0(5.71%),620 with stage I(30.00%),966 with stage II(46.73%),217 with stage III(10.50%),and 8 with stage IV(0.39%).Patients with breast cancer had significantly higher serum SEMA4C levels than benign breast tumor patients and normal controls(P<0.001).Elevated serum SEMA4C levels had AUC of 0.920(95%confidence interval[CI]:0.900–0.941)and 0.932(95%CI:0.911–0.953)for breast cancer detection in the two validation cohorts.The AUCs for detecting early-stage breast cancer(n=366)and ductal carcinoma in situ(n=85)were 0.931(95%CI:0.916–0.946)and 0.879(95%CI:0.832–0.925),respectively.Serum SEMA4C levels significantly decreased after surgery,and the reduction was more striking after modified radical mastectomy,compared with mass excision(P<0.001).The positive rate of enhanced serum SEMA4C levels was 84.77%for breast cancer and below 20.75%for the other 14 solid tumors.Conclusions:Serum SEMA4C demonstrated promising potential as a candidate biomarker for breast cancer diagnosis.However,validation in prospective settings and by other study groups is warranted.展开更多
Integration of human papillomavirus(HPV)DNA into the human genome is a reputed key driver of cervical cancer.However,the effects of HPV integration on chromatin structural organization and gene expression are largely ...Integration of human papillomavirus(HPV)DNA into the human genome is a reputed key driver of cervical cancer.However,the effects of HPV integration on chromatin structural organization and gene expression are largely unknown.We studied a cohort of 61 samples and identified an integration hot spot in the CCDC106 gene on chromosome 19.We then selected fresh cancer tissue that contained the unique integration loci at CCDC106 with no HPV episomal DNA and performed whole-genome,RNA,chromatin immunoprecipitation and high-throughput chromosome conformation capture(Hi-C)sequencing to identify the mechanisms of HPV integration in cervical carcinogenesis.Molecular analyses indicated that chromosome 19 exhibited significant genomic variation and differential expression densities,with correlation found between three-dimensional(3D)structural change and gene expression.Importantly,HPV integration divided one topologically associated domain(TAD)into two smaller TADs and hijacked an enhancer from PEG3 to CCDC106,with a decrease in PEG3 expression and an increase in CCDC106 expression.This expression dysregulation was further confirmed using 10 samples from our cohort,which exhibited the same HPV-CCDC106 integration.In summary,we found that HPV-CCDC106 integration altered local chromosome architecture and hijacked an enhancer via 3D genome structure remodeling.Thus,this study provides insight into the 3D structural mechanism underlying HPV integration in cervical carcinogenesis.展开更多
In 1979,De Giorgi conjectured that the only bounded monotone solutions to the Allen-Cahn equation △u+u-u^3=0 in R^N,are one-dimensional.This conjecture and its connection with minimal surfaces and Toda systems are th...In 1979,De Giorgi conjectured that the only bounded monotone solutions to the Allen-Cahn equation △u+u-u^3=0 in R^N,are one-dimensional.This conjecture and its connection with minimal surfaces and Toda systems are the subject of this survey article.展开更多
This paper presents a new family of solutions to the singularly perturbed Allen-Cahn equation α~2Δu + u(1- u^2) = 0 in a smooth bounded domain Ω R^3, with Neumann boundary condition and α > 0 a small paramete...This paper presents a new family of solutions to the singularly perturbed Allen-Cahn equation α~2Δu + u(1- u^2) = 0 in a smooth bounded domain Ω R^3, with Neumann boundary condition and α > 0 a small parameter. These solutions have the property that as α→ 0, their level sets collapse onto a bounded portion of a complete embedded minimal surface with finite total curvature intersecting ?Ω orthogonally and that is non-degenerate respect to ?Ω. The authors provide explicit examples of surfaces to which the result applies.展开更多
In this paper,we study the following N-coupled nonlinear Schrodinger system■,wheren≤3,N≥3,uj>0,βi,j=βj,i>0 are constants andβj,j=μj,j=1,...,N.There have been intensive studies for the system on existence/...In this paper,we study the following N-coupled nonlinear Schrodinger system■,wheren≤3,N≥3,uj>0,βi,j=βj,i>0 are constants andβj,j=μj,j=1,...,N.There have been intensive studies for the system on existence/non-existence and clas-sification of ground state solutions when N=2.However fewer results about the classification of ground state solution are available for N≥3.In this paper,we first give a complete classification result on ground state solutions with Morse indices 1,2 or 3 for three-coupled Schrodinger system.Then we generalize our results to N-coupled Schrodinger system for ground state solutions with Morse indices 1 and N.We show that any positive ground state solutions with Morse index 1 or Morse index N must be the form of(d1w,d2w,...,dNw)under suitable conditions,where w is the unique positive ground state solution of certain equation.Finally,we generalize our results to fractional N-coupled Schrödinger system.展开更多
基金National Science and Technology Major Sub-Project,Grant/Award Number:2018ZX10301402-002National Natural Science Foundation of China,Grant/Award Numbers:81772787,81902653,82072889+2 种基金Technical Innovation Special Project of Hubei Province,Grant/Award Number:2018ACA138Fundamental Research Funds for the Central Universities,Grant/Award Number:2019kfyXMBZ024Municipal Health Commission Project ofWuhan,Grant/Award Number:WX18Q16。
文摘Background:To date,there is no approved blood-based biomarker for breast cancer detection.Herein,we aimed to assess semaphorin 4C(SEMA4C),a pivotal protein involved in breast cancer progression,as a serum diagnostic biomarker.Methods:We included 6,213 consecutive inpatients from Tongji Hospital,Qilu Hospital,and Hubei Cancer Hospital.Training cohort and two validation cohorts were introduced for diagnostic exploration and validation.A pan-cancer cohort was used to independently explore the diagnostic potential of SEMA4C among solid tumors.Breast cancer patients who underwent mass excision prior to modified radical mastectomy were also analyzed.We hypothesized that increased pretreatment serum SEMA4C levels,measured using optimized in-house enzymelinked immunosorbent assay kits,could detect breast cancer.The endpoints were diagnostic performance,including area under the receiver operating characteristic curve(AUC),sensitivity,and specificity.Post-surgery pathological diagnosis was the reference standard and breast cancer staging followed the TNM classification.There was no restriction on disease stage for eligibilities.Results:We included 2667 inpatients with breast lesions,2378 patients with other solid tumors,and 1168 healthy participants.Specifically,118 patients with breast cancer were diagnosed with stage 0(5.71%),620 with stage I(30.00%),966 with stage II(46.73%),217 with stage III(10.50%),and 8 with stage IV(0.39%).Patients with breast cancer had significantly higher serum SEMA4C levels than benign breast tumor patients and normal controls(P<0.001).Elevated serum SEMA4C levels had AUC of 0.920(95%confidence interval[CI]:0.900–0.941)and 0.932(95%CI:0.911–0.953)for breast cancer detection in the two validation cohorts.The AUCs for detecting early-stage breast cancer(n=366)and ductal carcinoma in situ(n=85)were 0.931(95%CI:0.916–0.946)and 0.879(95%CI:0.832–0.925),respectively.Serum SEMA4C levels significantly decreased after surgery,and the reduction was more striking after modified radical mastectomy,compared with mass excision(P<0.001).The positive rate of enhanced serum SEMA4C levels was 84.77%for breast cancer and below 20.75%for the other 14 solid tumors.Conclusions:Serum SEMA4C demonstrated promising potential as a candidate biomarker for breast cancer diagnosis.However,validation in prospective settings and by other study groups is warranted.
基金supported by the National Natural Science Foundation of China(81630060 to P.W.,31771402 to G.L.,81830074 and 81772786 to H.W.,81572569 to G.C.,and 81772775 to J.W.)National Science and Technology Major Project(2019YFC1005202 and 2019YFC1005201 to K.L.,and 2018ZX10301402-002 to Q.G.)the research-oriented clinician funding program of Tongji Medical College,Huazhong University of Science and Technology for P.W
文摘Integration of human papillomavirus(HPV)DNA into the human genome is a reputed key driver of cervical cancer.However,the effects of HPV integration on chromatin structural organization and gene expression are largely unknown.We studied a cohort of 61 samples and identified an integration hot spot in the CCDC106 gene on chromosome 19.We then selected fresh cancer tissue that contained the unique integration loci at CCDC106 with no HPV episomal DNA and performed whole-genome,RNA,chromatin immunoprecipitation and high-throughput chromosome conformation capture(Hi-C)sequencing to identify the mechanisms of HPV integration in cervical carcinogenesis.Molecular analyses indicated that chromosome 19 exhibited significant genomic variation and differential expression densities,with correlation found between three-dimensional(3D)structural change and gene expression.Importantly,HPV integration divided one topologically associated domain(TAD)into two smaller TADs and hijacked an enhancer from PEG3 to CCDC106,with a decrease in PEG3 expression and an increase in CCDC106 expression.This expression dysregulation was further confirmed using 10 samples from our cohort,which exhibited the same HPV-CCDC106 integration.In summary,we found that HPV-CCDC106 integration altered local chromosome architecture and hijacked an enhancer via 3D genome structure remodeling.Thus,this study provides insight into the 3D structural mechanism underlying HPV integration in cervical carcinogenesis.
基金partially supported by Natural Sciences and Engineering Research Council of Canada
文摘In 1979,De Giorgi conjectured that the only bounded monotone solutions to the Allen-Cahn equation △u+u-u^3=0 in R^N,are one-dimensional.This conjecture and its connection with minimal surfaces and Toda systems are the subject of this survey article.
基金supported by the Grant 13-00863S of the Grant Agency of the Czech Republicgrants Fondecyt 1150066,Fondo Basal CMM,Millenium+1 种基金Nucleus CAPDE NC130017NSERC accelerator
文摘This paper presents a new family of solutions to the singularly perturbed Allen-Cahn equation α~2Δu + u(1- u^2) = 0 in a smooth bounded domain Ω R^3, with Neumann boundary condition and α > 0 a small parameter. These solutions have the property that as α→ 0, their level sets collapse onto a bounded portion of a complete embedded minimal surface with finite total curvature intersecting ?Ω orthogonally and that is non-degenerate respect to ?Ω. The authors provide explicit examples of surfaces to which the result applies.
文摘In this paper,we study the following N-coupled nonlinear Schrodinger system■,wheren≤3,N≥3,uj>0,βi,j=βj,i>0 are constants andβj,j=μj,j=1,...,N.There have been intensive studies for the system on existence/non-existence and clas-sification of ground state solutions when N=2.However fewer results about the classification of ground state solution are available for N≥3.In this paper,we first give a complete classification result on ground state solutions with Morse indices 1,2 or 3 for three-coupled Schrodinger system.Then we generalize our results to N-coupled Schrodinger system for ground state solutions with Morse indices 1 and N.We show that any positive ground state solutions with Morse index 1 or Morse index N must be the form of(d1w,d2w,...,dNw)under suitable conditions,where w is the unique positive ground state solution of certain equation.Finally,we generalize our results to fractional N-coupled Schrödinger system.