Introduction Primary liver cancer,the second most common cause of cancer related death worldwide1,presents ethnic,etiological,sex,and geographical diversity2(Figure 1A).At the histological level,liver cancer includes ...Introduction Primary liver cancer,the second most common cause of cancer related death worldwide1,presents ethnic,etiological,sex,and geographical diversity2(Figure 1A).At the histological level,liver cancer includes two major types:hepatocellular carcinoma(HCC,about 80%)and cholangiocarcinoma(CCA,about 15%).Many etiological factors contribute to HCC development,such as hepatitis展开更多
Objective:MicroRNAs(miRNAs)have a large number of biologically diverse effects including cell development and tumorigenesis.Tumor-initiating cells or cancer stem cells(CSCs)are responsible for tumor initiation,tumor m...Objective:MicroRNAs(miRNAs)have a large number of biologically diverse effects including cell development and tumorigenesis.Tumor-initiating cells or cancer stem cells(CSCs)are responsible for tumor initiation,tumor metastasis,and recurrence after tumor resection.Until now,many cell surface biomarkers have been used to isolate CSCs,such as EpCAM.展开更多
p53 mutations have been found in many esophageal squamous cell carcinoma(ESCC)clinical specimens and cell lines.We reasoned that functional inactivation of wild-type p53 or the functional activation of mutant-type p53...p53 mutations have been found in many esophageal squamous cell carcinoma(ESCC)clinical specimens and cell lines.We reasoned that functional inactivation of wild-type p53 or the functional activation of mutant-type p53 might exist in these specimens and cell lines.In this study,we identified the correlation between p53 functional activation and its genotype infive different ESCC cell lines.To examine the potential p53 activation in a certain ESCC cell line,DNA damage methods including X-ray exposure and cisplatin treatment were employed to treat cells.Further,the expression of p53 protein and four transcripts of well-known p53 target genes were investi-gated using Western blot and reverse transcription-polymerase chain reaction(RT-PCR)after cell exposure to DNA damage.The results showed that in KYSE 30 cell line with mutant p53 and KYSE 150 with wild-type p53,p53 could be activated by DNA damages.However,p53 could not be activated following the DNA damages in YES 2 with wild-type p53,KYSE 70 with mutant p53,and EC9706 with unknown p53 genotype.All our data indicated that p53 function in certain cells is not closely correlated with its genotype.To judge p53 function in a particular cell line,it is important to examine the p53 functional activation,but not to simply rely on the p53 genotype.展开更多
基金supported,in part,by the Precision Medical Research Program from Ministry of Science and Technology of China(Grant No.YL 2017YFC0908400)National Science and Technology Major Project for Infectious Disease and Funding(Grant No.YL 17-163-12-ZT-005-095-01)+2 种基金Science and Technology Commission in Ministry of National Defense of China(Grant No.YL 17-163-12-ZT-005-095-01)Xinwei Wang was supported by the intramural research program of the Center for Cancer Research,National Cancer Institute of the United StatesJunfang Ji was supported by the Thousand Young Talents Plan of China,National Natural Science Foundation of China(Grant No.81672905)
文摘Introduction Primary liver cancer,the second most common cause of cancer related death worldwide1,presents ethnic,etiological,sex,and geographical diversity2(Figure 1A).At the histological level,liver cancer includes two major types:hepatocellular carcinoma(HCC,about 80%)and cholangiocarcinoma(CCA,about 15%).Many etiological factors contribute to HCC development,such as hepatitis
文摘Objective:MicroRNAs(miRNAs)have a large number of biologically diverse effects including cell development and tumorigenesis.Tumor-initiating cells or cancer stem cells(CSCs)are responsible for tumor initiation,tumor metastasis,and recurrence after tumor resection.Until now,many cell surface biomarkers have been used to isolate CSCs,such as EpCAM.
基金supported in part by the National Basic Research Program of China(2002CB513101)the National Natural Science Foundation of China(30225018 and 30400074)the Beijing Natural Science Foundation(7043074).
文摘p53 mutations have been found in many esophageal squamous cell carcinoma(ESCC)clinical specimens and cell lines.We reasoned that functional inactivation of wild-type p53 or the functional activation of mutant-type p53 might exist in these specimens and cell lines.In this study,we identified the correlation between p53 functional activation and its genotype infive different ESCC cell lines.To examine the potential p53 activation in a certain ESCC cell line,DNA damage methods including X-ray exposure and cisplatin treatment were employed to treat cells.Further,the expression of p53 protein and four transcripts of well-known p53 target genes were investi-gated using Western blot and reverse transcription-polymerase chain reaction(RT-PCR)after cell exposure to DNA damage.The results showed that in KYSE 30 cell line with mutant p53 and KYSE 150 with wild-type p53,p53 could be activated by DNA damages.However,p53 could not be activated following the DNA damages in YES 2 with wild-type p53,KYSE 70 with mutant p53,and EC9706 with unknown p53 genotype.All our data indicated that p53 function in certain cells is not closely correlated with its genotype.To judge p53 function in a particular cell line,it is important to examine the p53 functional activation,but not to simply rely on the p53 genotype.
