Intelligent drug delivery system based on “stimulus-response”mode emerging a promising perspective in next generation lipidbased nanoparticle.Here,we classify signal sources into physical and physiological stimulati...Intelligent drug delivery system based on “stimulus-response”mode emerging a promising perspective in next generation lipidbased nanoparticle.Here,we classify signal sources into physical and physiological stimulation according to their origin.展开更多
The Coronavirus disease 2019(COVID-19)has posed a serious threat to global health and the world economy.Antiviral therapies targeting coronavirus are urgently required.The Cepharanthine(CEP)is a traditional Chinese he...The Coronavirus disease 2019(COVID-19)has posed a serious threat to global health and the world economy.Antiviral therapies targeting coronavirus are urgently required.The Cepharanthine(CEP)is a traditional Chinese herbal extract.Our previous research revealed that CEP has a very potent anti-coronavirus effect,but its mechanism of action was not fully understood.To investigate the effect of novel coronavirus on protein glycosylation in infected cells and to further investigate the mechanism of action of CEP against coronavirus,a cellular model using coronavirus GX_P2V infection of Vero E6 cells was established.The effect of coronavirus GX_P2V on host cell protein glycosylation was investigated by N-glycoproteomic analysis,and the antagonistic effect of CEP on the abnormal protein glycosylation caused by coronavirus was analyzed.The results showed that GX_P2V could cause abnormal changes in protein glycosylation levels in host cells,while CEP could partially antagonize the abnormal protein glycosylation caused by GX_P2V.In addition,we also found that CEP could regulate the glycosylation level of coronavirus S protein.In conclusion,this article provides important ideas about the infection mechanism of novel coronaviruses,providing evidence for CEP as a promising therapeutic option for coronavirus infection.展开更多
基金This work was supported by Beijing Natural Science Foundation(7214283)the National Key Research&Development Program of China(grant Nos.2021YFA1201000 and 2018YFE0117800)+3 种基金National Natural Science Foundation of China(NSFC)Key Project(grant No.32030060)NSFC International CollaborationKey Project(grant No.51861135103)The authors also appreciate the support by the Beijing-Tianjin-Hebei Basic Research Cooperation Project(19JCZDJC64100)This study was also supported by the Science and Technology Development Fund,Macao SAR(File no.0124/2019/A3).
文摘Intelligent drug delivery system based on “stimulus-response”mode emerging a promising perspective in next generation lipidbased nanoparticle.Here,we classify signal sources into physical and physiological stimulation according to their origin.
基金the National Key Research and Development Program of China(2018YFA0903000)the National Natural Science Foundation of China(81672001)+2 种基金China MoST Emergency Project on COVID-19(2020YFC0840800)Guangdong Basic and Applied Basic Research Foundation(2021A1515110619)the Fundamental Research Funds for the Central Universities(KG16052901).
文摘The Coronavirus disease 2019(COVID-19)has posed a serious threat to global health and the world economy.Antiviral therapies targeting coronavirus are urgently required.The Cepharanthine(CEP)is a traditional Chinese herbal extract.Our previous research revealed that CEP has a very potent anti-coronavirus effect,but its mechanism of action was not fully understood.To investigate the effect of novel coronavirus on protein glycosylation in infected cells and to further investigate the mechanism of action of CEP against coronavirus,a cellular model using coronavirus GX_P2V infection of Vero E6 cells was established.The effect of coronavirus GX_P2V on host cell protein glycosylation was investigated by N-glycoproteomic analysis,and the antagonistic effect of CEP on the abnormal protein glycosylation caused by coronavirus was analyzed.The results showed that GX_P2V could cause abnormal changes in protein glycosylation levels in host cells,while CEP could partially antagonize the abnormal protein glycosylation caused by GX_P2V.In addition,we also found that CEP could regulate the glycosylation level of coronavirus S protein.In conclusion,this article provides important ideas about the infection mechanism of novel coronaviruses,providing evidence for CEP as a promising therapeutic option for coronavirus infection.
