Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion(Hg(II))transport.However,they cannot reliably simulate the human intestinal microenvironment and m...Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion(Hg(II))transport.However,they cannot reliably simulate the human intestinal microenvironment and monitor cellular physiology in situ;thus,the mechanism of Hg(II)transport in the human intestine is still unclear.Here,a gut-on-a-chip integrated with transepithelial electrical resistance(TEER)sensors and electrochemical sensors is proposed for dynamically simulating the formation of the physical intestinal barrier and monitoring the transport and absorption of Hg(II)in situ.The cellular microenvironment was recreated by applying fluid shear stress(0.02 dyne/cm^(2))and cyclic mechanical strain(1%,0.15 Hz).Hg(II)absorption and physical damage to cells were simultaneously monitored by electrochemical and TEER sensors when intestinal epithelial cells were exposed to different concentrations of Hg(II)mixed in culture medium.Hg(II)absorption increased by 23.59%when tensile strain increased from 1%to 5%,and the corresponding expression of Piezo1 and DMT1 on the cell surface was upregulated.展开更多
基金supported by the Taishan Scholars Program of Shandong Province (No.tsqn201812087)the National Natural Science Foundation of China (No.61901239)+1 种基金Qingchuang Science and Technology Plan Foundation for Colleges and Universities in Shandong Province (No.2019KJB009)the Young Ph.D.Cooperation Foundation of Qilu University of Technology (Shandong Academy of Sciences) (No.2019BSHZ002).
文摘Animal models and static cultures of intestinal epithelial cells are commonly used platforms for exploring mercury ion(Hg(II))transport.However,they cannot reliably simulate the human intestinal microenvironment and monitor cellular physiology in situ;thus,the mechanism of Hg(II)transport in the human intestine is still unclear.Here,a gut-on-a-chip integrated with transepithelial electrical resistance(TEER)sensors and electrochemical sensors is proposed for dynamically simulating the formation of the physical intestinal barrier and monitoring the transport and absorption of Hg(II)in situ.The cellular microenvironment was recreated by applying fluid shear stress(0.02 dyne/cm^(2))and cyclic mechanical strain(1%,0.15 Hz).Hg(II)absorption and physical damage to cells were simultaneously monitored by electrochemical and TEER sensors when intestinal epithelial cells were exposed to different concentrations of Hg(II)mixed in culture medium.Hg(II)absorption increased by 23.59%when tensile strain increased from 1%to 5%,and the corresponding expression of Piezo1 and DMT1 on the cell surface was upregulated.
