Isolation and expression analysis of CBF4 from <i>Vitis amurensis</i>associated with stress

The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript accumulation of V. amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis-elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.

Status Quo and Countermeasures of Sustainable Development for Zhenjiang Wanshan Hongbian Modern Agricultural Science Park

In this paper,status quo of development in Zhenjiang Wanshan Hongbian Modern Agricultural Science Park is studied,and some problems are pointed out,such as unreasonable staffing structure and weak self-financing ability.Talent introduction strategy and branding and standardization strategy are proposed,and financing channels should be widened.Meanwhile,agricultural production structure should be adjusted,and science and technology services should be promoted.The research aims to provide the reference for sustainable development of similar modern agricultural science park in China.

Research Progress on the Development of Modern Agricultural Science and Technology Park

This paper summarizes the typical models,construction theory and development status of modern agricultural science and technology parks at home and abroad,in order to provide a reference for the sustainable development of modern agricultural science and technology parks in China.It is found that at present,the agricultural science and technology park in China is in the stage of quantitative development,neglecting the qualitative construction of the park.This paper points out that the balanced relationship among ecology,economy and society should be adjusted to promote the sustainable development of the park.

3个小型高原湖泊水陆交错带中植物叶片pH值变异及其潜在影响因素

陆生植物叶片pH值是与植物生理功能和养分利用状况息息相关的重要植物属性,并随植物功能型和环境的变化而变化。然而,水生植物叶片pH值的变异特征及其与陆生植物间的差异性仍不清楚。在3个自然形成的小型高原湖泊的湖滨植物群落中,我们采集植物、土壤和湖水样品,检测了植物叶片pH值与碳、氮含量以及环境条件(水或土壤pH值、土壤水位状况)。从植物功能型和群落两个水平分析了湖滨植物叶pH值沿水分梯度的变化及其潜在影响因素。研究结果表明,不同类型的水生植物,以及水生、沼生和陆生环境中生长的植物的叶pH存在较大差异;浮叶植物叶片pH值(4.21±0.05)显著低于挺水植物(5.71±0.07)和沉水植物(5.82±0.06);水生草本植物叶片pH值(5.43±0.10)显著低于沼生草本植物(6.12±0.07)和陆生草本植物(5.74±0.05);陆生草本植物叶片pH值显著高于木本植物。植物功能群间叶片pH值的变异可能与叶片的结构、光利用特性和养分特性有关。群落水平的结果与植物功能型水平相一致:水生群落叶片pH值显著低于陆生群落。群落水平叶片pH值可能主要受物种组成、水分条件和环境pH值的影响。本研究首次探索了水生植物的pH值,并且在景观尺度上对比了不同功能型植物的叶片pH值,为深入探索植物pH值变异机制以及其在水生和湿地生态系统中的生态意义提供参考。