[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different ca...[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.展开更多
High-speed maglev trains will play an important role in the high-speed transportation system in the near future.However,under the conditions of strong magnetic fields and continuous operation,the actuators of the high...High-speed maglev trains will play an important role in the high-speed transportation system in the near future.However,under the conditions of strong magnetic fields and continuous operation,the actuators of the high-speed maglev train suspension system are prone to lose partial effectiveness,which makes the suspension control problem challenging.In addition,most existing fault-tolerant control(FTC)methods for suspension systems require linearization around the equilibrium points during the controller design or stability analysis.Therefore,from a practical perspective,this study presents a novel nonlinear FTC strategy with adaptive compensation for high-speed maglev train suspension systems.First,a nonlinear dynamic model of the suspension system based on join-structure is established and the actuator failures are described.Then,a nonlinear fault-tolerant suspension control law with an adaptive update law is designed to achieve stable suspension against partial actuator failure.The Lyapunov theory and extended Barbalat lemma are utilized to rigorously prove the closed-loop asymptotic stability even if there is partial actuator failure,without any approximation to the original nonlinear dynamics.Finally,hardware experimental results are included to demonstrate the effectiveness of the proposed approach.展开更多
Steroidal drugs have wide indications such as anti-inflammation, anti-tumor, endocrine regulation,fertility management. Phytosterol is the main starting materials for the industrial synthesis of steroid drugs. Microbi...Steroidal drugs have wide indications such as anti-inflammation, anti-tumor, endocrine regulation,fertility management. Phytosterol is the main starting materials for the industrial synthesis of steroid drugs. Microbial transformation of phytosterol is a simple and environmentally friendly process. Efficient microbial strains for industrial phytosterol transformation are critical for the commercial success. To this end, a 96-well plate based method was developed to discriminate the mixture of 4-androstene-3,17-dione, androsta-1,4-diene-3,17-dione, and bisnoraldehyde, with different ratio of the 3 components in the mixture, which mimics the sterol bioconversion products by using the Mycobacterium neoaurum. The M. neoaurum bioconversed broth test using phytosterol as substrate also found that the spectrum methodology can evaluate the relative content of different compounds. The method is practical, high throughput and can replace the conventional HPLC-based assay for rapid selection of desired microbial strains.展开更多
基金Supported by"Six Talents Peaks"Project in Jiangsu Province in 2015(NY-024)Project of Jiangsu Polytechnic College of Agriculture and Forestry(2014KJ28)
文摘[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.
基金supported by the National Natural Science Foundation of China(Nos.52272374 and 52072269)the Shanghai Soft Science Research Project(No.22692194800)the Fundamental Research Funds for the Central Universities,China.
文摘High-speed maglev trains will play an important role in the high-speed transportation system in the near future.However,under the conditions of strong magnetic fields and continuous operation,the actuators of the high-speed maglev train suspension system are prone to lose partial effectiveness,which makes the suspension control problem challenging.In addition,most existing fault-tolerant control(FTC)methods for suspension systems require linearization around the equilibrium points during the controller design or stability analysis.Therefore,from a practical perspective,this study presents a novel nonlinear FTC strategy with adaptive compensation for high-speed maglev train suspension systems.First,a nonlinear dynamic model of the suspension system based on join-structure is established and the actuator failures are described.Then,a nonlinear fault-tolerant suspension control law with an adaptive update law is designed to achieve stable suspension against partial actuator failure.The Lyapunov theory and extended Barbalat lemma are utilized to rigorously prove the closed-loop asymptotic stability even if there is partial actuator failure,without any approximation to the original nonlinear dynamics.Finally,hardware experimental results are included to demonstrate the effectiveness of the proposed approach.
文摘Steroidal drugs have wide indications such as anti-inflammation, anti-tumor, endocrine regulation,fertility management. Phytosterol is the main starting materials for the industrial synthesis of steroid drugs. Microbial transformation of phytosterol is a simple and environmentally friendly process. Efficient microbial strains for industrial phytosterol transformation are critical for the commercial success. To this end, a 96-well plate based method was developed to discriminate the mixture of 4-androstene-3,17-dione, androsta-1,4-diene-3,17-dione, and bisnoraldehyde, with different ratio of the 3 components in the mixture, which mimics the sterol bioconversion products by using the Mycobacterium neoaurum. The M. neoaurum bioconversed broth test using phytosterol as substrate also found that the spectrum methodology can evaluate the relative content of different compounds. The method is practical, high throughput and can replace the conventional HPLC-based assay for rapid selection of desired microbial strains.
