<strong>Objective:</strong><span style="font-family:""><span style="font-family:Verdana;"> Breast Cancer (BC) is characterized by high complexity and heterogeneity, and...<strong>Objective:</strong><span style="font-family:""><span style="font-family:Verdana;"> Breast Cancer (BC) is characterized by high complexity and heterogeneity, and microRNA (miRNA) is bound up with the occurrence and </span><span style="font-family:Verdana;">development of BC. In this study, we evaluated the prognostic value of</span><span style="font-family:Verdana;"> miR</span><span><span style="font-family:Verdana;">NA in BC. </span><b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:Verdana;"> Breast ductal and lobular cancers are the most </span></span><span style="font-family:Verdana;">common types of Breast Carcinomas (BC) and indicate the high complexity heterogeneity in this disease. Each BC patient has unique morphological and </span><span style="font-family:Verdana;">molecular features. MicroRNAs (miRNAs) play a critical role in human </span><span style="font-family:Verdana;">oncoge</span><span style="font-family:Verdana;">nesis, progression, and prognosis. Our study aimed to identify potential</span><span style="font-family:Verdana;"> prognostic biomarkers of breast ductal and lobular cancers to predict the overall </span><span><span style="font-family:Verdana;">survival outcome. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> All analyzed miRNA sequencing and clinical </span></span><span style="font-family:Verdana;">data were obtained from the Genomic Data Commons Data Porta. edgeR package in R </span><span style="font-family:Verdana;">software was used to analyze the differential miRNA expression profiles</span><span style="font-family:Verdana;">. Com</span><span style="font-family:Verdana;">plete survival information and differentially expressed miRNA expression</span><span style="font-family:Verdana;"> were obtained and the Caret package was used for random division of the samples along with their profiles into two groups (training group and test group). We </span><span style="font-family:Verdana;">performed univariate Cox regression analyses for miRNAs in the training</span><span> </span><span style="font-family:Verdana;">group. We utilized three different web-based tools to identify the targe</span><span style="font-family:Verdana;">t genes </span><span style="font-family:Verdana;">of miRNAs and used the Perl language to evaluate the target genes for</span><span><span style="font-family:Verdana;"> miRNA signature. STRING database was used to assess PPIs. </span><b><span style="font-family:Verdana;">Re</span></b></span><b><span style="font-family:Verdana;">sults:</span></b><span style="font-family:Verdana;"> A total of </span><span style="font-family:Verdana;">304 differentially expressed miRNAs were identified (213 were upregulated </span><span style="font-family:Verdana;">and 91 were downregulated). Among these, nine (hsa-miR-204-5p, hsa-miR-7706, hsa-miR-1247-3p, hsa-miR-20b-3p, hsa-miR-605-5p, hsa-</span><span style="font-family:Verdana;">miR-615-3p, hsa-miR-4652-5p, hsa-miR-9-5p, hsa-miR-2115-5p) </span><span style="font-family:Verdana;">miRNAs </span><span style="font-family:Verdana;">which remarkably correlated with overall survival rate of BC were per</span><span style="font-family:Verdana;">formed by Cox regression analysis and miRNA signature risk score built. And then </span><span style="font-family:Verdana;">we performed the model of BC patients for three years survival risk, the</span><span style="font-family:Verdana;"> AUCs of ROC were 0.804, 0.667, and 0.739 in the training, test, and entire groups, respectively. miRNAs were differentially expressed in tumor-related </span><span style="font-family:Verdana;">biological processes and pathways by functional enrichment and</span><span style="font-family:Verdana;"> bioinformatic analysis. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The current study provided novel insights into the mi</span><span style="font-family:Verdana;">RNA-based mRNA network in BC. The nine miRNA and ten hub genes </span><span style="font-family:Verdana;">may be independent prognostic signatures for survival prediction in BC patients.</span></span>展开更多
Intra porous fibrous membranes have enhanced metal ionic adsorption and oil separation abilities than those of intra nonporous fibrous membrane.In this paper,we prepared highly intra porous fibrous poly(vinylidene flu...Intra porous fibrous membranes have enhanced metal ionic adsorption and oil separation abilities than those of intra nonporous fibrous membrane.In this paper,we prepared highly intra porous fibrous poly(vinylidene fluoride)(PVDF)membranes using an innovated water-mediated electrospinning approach.FTIR-ATR and XRD techniques confirmed the conversion of non-polarα-phase to polarβ-phase in electrospun membranes.The porous fibrous membrane M–16 had adsorbed oil almost 120%and metal adsorption around 15%,12%,5%,13%respectively for Pb^(2+),Cd^(2+),Cu^(2+)and Zn^(2+),which are larger than the counterpart of nonporous M–18.The nonporous fibrous membranes have better peak to peak output voltage(Vp-Vp)2 to 3 times than the porous fibrous membranes(M–16).The results show apparent potential applications in wastewater/oil spill treatment as well as piezoelectric sensors.展开更多
文摘<strong>Objective:</strong><span style="font-family:""><span style="font-family:Verdana;"> Breast Cancer (BC) is characterized by high complexity and heterogeneity, and microRNA (miRNA) is bound up with the occurrence and </span><span style="font-family:Verdana;">development of BC. In this study, we evaluated the prognostic value of</span><span style="font-family:Verdana;"> miR</span><span><span style="font-family:Verdana;">NA in BC. </span><b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:Verdana;"> Breast ductal and lobular cancers are the most </span></span><span style="font-family:Verdana;">common types of Breast Carcinomas (BC) and indicate the high complexity heterogeneity in this disease. Each BC patient has unique morphological and </span><span style="font-family:Verdana;">molecular features. MicroRNAs (miRNAs) play a critical role in human </span><span style="font-family:Verdana;">oncoge</span><span style="font-family:Verdana;">nesis, progression, and prognosis. Our study aimed to identify potential</span><span style="font-family:Verdana;"> prognostic biomarkers of breast ductal and lobular cancers to predict the overall </span><span><span style="font-family:Verdana;">survival outcome. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> All analyzed miRNA sequencing and clinical </span></span><span style="font-family:Verdana;">data were obtained from the Genomic Data Commons Data Porta. edgeR package in R </span><span style="font-family:Verdana;">software was used to analyze the differential miRNA expression profiles</span><span style="font-family:Verdana;">. Com</span><span style="font-family:Verdana;">plete survival information and differentially expressed miRNA expression</span><span style="font-family:Verdana;"> were obtained and the Caret package was used for random division of the samples along with their profiles into two groups (training group and test group). We </span><span style="font-family:Verdana;">performed univariate Cox regression analyses for miRNAs in the training</span><span> </span><span style="font-family:Verdana;">group. We utilized three different web-based tools to identify the targe</span><span style="font-family:Verdana;">t genes </span><span style="font-family:Verdana;">of miRNAs and used the Perl language to evaluate the target genes for</span><span><span style="font-family:Verdana;"> miRNA signature. STRING database was used to assess PPIs. </span><b><span style="font-family:Verdana;">Re</span></b></span><b><span style="font-family:Verdana;">sults:</span></b><span style="font-family:Verdana;"> A total of </span><span style="font-family:Verdana;">304 differentially expressed miRNAs were identified (213 were upregulated </span><span style="font-family:Verdana;">and 91 were downregulated). Among these, nine (hsa-miR-204-5p, hsa-miR-7706, hsa-miR-1247-3p, hsa-miR-20b-3p, hsa-miR-605-5p, hsa-</span><span style="font-family:Verdana;">miR-615-3p, hsa-miR-4652-5p, hsa-miR-9-5p, hsa-miR-2115-5p) </span><span style="font-family:Verdana;">miRNAs </span><span style="font-family:Verdana;">which remarkably correlated with overall survival rate of BC were per</span><span style="font-family:Verdana;">formed by Cox regression analysis and miRNA signature risk score built. And then </span><span style="font-family:Verdana;">we performed the model of BC patients for three years survival risk, the</span><span style="font-family:Verdana;"> AUCs of ROC were 0.804, 0.667, and 0.739 in the training, test, and entire groups, respectively. miRNAs were differentially expressed in tumor-related </span><span style="font-family:Verdana;">biological processes and pathways by functional enrichment and</span><span style="font-family:Verdana;"> bioinformatic analysis. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> The current study provided novel insights into the mi</span><span style="font-family:Verdana;">RNA-based mRNA network in BC. The nine miRNA and ten hub genes </span><span style="font-family:Verdana;">may be independent prognostic signatures for survival prediction in BC patients.</span></span>
基金This work was supported by the Natural Science Foundation of China(Grant No.51372042,51872053)Guangdong Provincial Natural Science Foundation(2015A030308004)+1 种基金the NSFCGuangdong Joint Fund(Grant No.U1501246)the Dongguan City Frontier Research Project(2019622101006)and the Advanced Energy Science and Technology Guangdong Provincial Laboratory Foshan Branch-Foshan Xianhu Laboratory Open Fund-Key Project(Grant No.XHT2020-011).
文摘Intra porous fibrous membranes have enhanced metal ionic adsorption and oil separation abilities than those of intra nonporous fibrous membrane.In this paper,we prepared highly intra porous fibrous poly(vinylidene fluoride)(PVDF)membranes using an innovated water-mediated electrospinning approach.FTIR-ATR and XRD techniques confirmed the conversion of non-polarα-phase to polarβ-phase in electrospun membranes.The porous fibrous membrane M–16 had adsorbed oil almost 120%and metal adsorption around 15%,12%,5%,13%respectively for Pb^(2+),Cd^(2+),Cu^(2+)and Zn^(2+),which are larger than the counterpart of nonporous M–18.The nonporous fibrous membranes have better peak to peak output voltage(Vp-Vp)2 to 3 times than the porous fibrous membranes(M–16).The results show apparent potential applications in wastewater/oil spill treatment as well as piezoelectric sensors.
