"Surplus income" of farmers has been increasing steadily with the marked improvement of rural economy. However,development of rural financing market in China is rather backward. To satisfy the financing requ..."Surplus income" of farmers has been increasing steadily with the marked improvement of rural economy. However,development of rural financing market in China is rather backward. To satisfy the financing requirement of farmers and meet the demand of the construction of new countryside as well as harmonious society,development of financing market in rural area is eager to be quickened. Taking Guangxi Autonomous Region as an example,there are problems in rural investing and financing development. Firstly,farmers are in lacking of accurate understanding of investing and financing. Secondly,investors in rural area lack professional knowledge about financing generally. Thirdly,rural area has underdeveloped information degree as well as imperfect investing and financing environment. Fourthly,there are no financial products developed for rural area. Fifthly,economic development is unbalanced and relatively underdeveloped in rural area. Lastly,rural financial market has long been neglected by financial intermediaries. In order to cope with these problems,firstly,farmers should be assisted to establish accurate financial awareness and master necessary financial knowledge. Secondly,local intermediaries like securities firms and banks should be encouraged to exert impact on rural financial market. Thirdly,financial products suitable for Guangxi rural area are to be developed. Fourthly, construction and perfection of rural financial market should be quickened. Lastly,rural economic development should be quickened to enlarge capital source of financing.展开更多
目的:探讨TGF-β1通过调控脂肪酸合酶对胃癌细胞迁移及侵袭的影响。方法:收集南方医科大学顺德医院2016年1月至2018年12月存档的胃癌手术切除的石蜡标本54例,采用免疫组织化学法检测人胃癌组织中转化生长因子β1(transforminggrowth fac...目的:探讨TGF-β1通过调控脂肪酸合酶对胃癌细胞迁移及侵袭的影响。方法:收集南方医科大学顺德医院2016年1月至2018年12月存档的胃癌手术切除的石蜡标本54例,采用免疫组织化学法检测人胃癌组织中转化生长因子β1(transforminggrowth factorβ1,TGF-β1)及脂肪酸合成酶(fatty acid synthetase,FASN)的表达情况,分析两者之间的相关性。构建TGF-β1基因过表达及si RNA沉默载体转染胃癌细胞株,用Real-time PCR和Western blot法分别检测对FASN m RNA及蛋白表达的影响。利用si RNA行FASN做基因沉默后,采用划痕及Transwell实验检测对胃癌细胞迁移及侵袭能力的影响。结果:免疫组织化学结果显示,TGF-β1与FASN在胃癌组织中的表达呈显著正相关。转染TGF-β1过表达质粒能明显上调FASN m RNA及蛋白的表达水平。反之,TGF-β1 si RNA则明显抑制FASN的表达。共转染FASN si RNA后,能减弱TGF-β1引起的N-cadherin蛋白表达、增强E-cadherin蛋白表达;划痕及Transwell实验显示能明显减弱TGF-β1所上调的细胞迁移及侵袭能力。结论:FASN在TGF-β1调控的胃癌细胞的迁移及侵袭过程中发挥重要作用。展开更多
文摘"Surplus income" of farmers has been increasing steadily with the marked improvement of rural economy. However,development of rural financing market in China is rather backward. To satisfy the financing requirement of farmers and meet the demand of the construction of new countryside as well as harmonious society,development of financing market in rural area is eager to be quickened. Taking Guangxi Autonomous Region as an example,there are problems in rural investing and financing development. Firstly,farmers are in lacking of accurate understanding of investing and financing. Secondly,investors in rural area lack professional knowledge about financing generally. Thirdly,rural area has underdeveloped information degree as well as imperfect investing and financing environment. Fourthly,there are no financial products developed for rural area. Fifthly,economic development is unbalanced and relatively underdeveloped in rural area. Lastly,rural financial market has long been neglected by financial intermediaries. In order to cope with these problems,firstly,farmers should be assisted to establish accurate financial awareness and master necessary financial knowledge. Secondly,local intermediaries like securities firms and banks should be encouraged to exert impact on rural financial market. Thirdly,financial products suitable for Guangxi rural area are to be developed. Fourthly, construction and perfection of rural financial market should be quickened. Lastly,rural economic development should be quickened to enlarge capital source of financing.
文摘目的:探讨TGF-β1通过调控脂肪酸合酶对胃癌细胞迁移及侵袭的影响。方法:收集南方医科大学顺德医院2016年1月至2018年12月存档的胃癌手术切除的石蜡标本54例,采用免疫组织化学法检测人胃癌组织中转化生长因子β1(transforminggrowth factorβ1,TGF-β1)及脂肪酸合成酶(fatty acid synthetase,FASN)的表达情况,分析两者之间的相关性。构建TGF-β1基因过表达及si RNA沉默载体转染胃癌细胞株,用Real-time PCR和Western blot法分别检测对FASN m RNA及蛋白表达的影响。利用si RNA行FASN做基因沉默后,采用划痕及Transwell实验检测对胃癌细胞迁移及侵袭能力的影响。结果:免疫组织化学结果显示,TGF-β1与FASN在胃癌组织中的表达呈显著正相关。转染TGF-β1过表达质粒能明显上调FASN m RNA及蛋白的表达水平。反之,TGF-β1 si RNA则明显抑制FASN的表达。共转染FASN si RNA后,能减弱TGF-β1引起的N-cadherin蛋白表达、增强E-cadherin蛋白表达;划痕及Transwell实验显示能明显减弱TGF-β1所上调的细胞迁移及侵袭能力。结论:FASN在TGF-β1调控的胃癌细胞的迁移及侵袭过程中发挥重要作用。
