A maize variety,Huatian-1,had an unusuallylow translocation rate of cadmium(Cd)(59.6 mg·kg^(-1)inthe roots and 0.093 mg·kg^(-1)in the grain)compared to24 other varieties while being grown in soils with16.50 ...A maize variety,Huatian-1,had an unusuallylow translocation rate of cadmium(Cd)(59.6 mg·kg^(-1)inthe roots and 0.093 mg·kg^(-1)in the grain)compared to24 other varieties while being grown in soils with16.50 mg·kg^(-1)Cd.This indicates that this particularspecies may have special mechanisms that affect theabsorption and translocation pattern of Cd.In this paper,the technique of suppression subtractive hybridization(SSH)was used to isolate and identify Cd-induced genesfrom Huatian-1 hydroponically exposed to 0.1mMCdCl_(2) for 1 h,12 h,24 h,and 48 h.We found a total of15 differentially expressed genes in the four groups;2,3,4,and 6 genes were from the groups of 1 h,12 h,24 h,and48 h treatment,respectively.Phospholipase PLDb1mRNA,adenosine triphosphate(ATP)phosphoribosyltransferase 2,and Sp17 were turned on in the maize inresponse to Cd stress,and it might provide new clues toexplain the mechanism of maize tolerance to Cd.展开更多
基金This research was supported by the National Natural Science Foundation of China(Grant No.20877104)the Natural Science Foundation of Guangdong Province(No.021686)the Research Foundation for Doctoral Programs of Chinese Universities(No.20020558004).
文摘A maize variety,Huatian-1,had an unusuallylow translocation rate of cadmium(Cd)(59.6 mg·kg^(-1)inthe roots and 0.093 mg·kg^(-1)in the grain)compared to24 other varieties while being grown in soils with16.50 mg·kg^(-1)Cd.This indicates that this particularspecies may have special mechanisms that affect theabsorption and translocation pattern of Cd.In this paper,the technique of suppression subtractive hybridization(SSH)was used to isolate and identify Cd-induced genesfrom Huatian-1 hydroponically exposed to 0.1mMCdCl_(2) for 1 h,12 h,24 h,and 48 h.We found a total of15 differentially expressed genes in the four groups;2,3,4,and 6 genes were from the groups of 1 h,12 h,24 h,and48 h treatment,respectively.Phospholipase PLDb1mRNA,adenosine triphosphate(ATP)phosphoribosyltransferase 2,and Sp17 were turned on in the maize inresponse to Cd stress,and it might provide new clues toexplain the mechanism of maize tolerance to Cd.
