Background: To determine the concentration of group IIA phospholipase A2 (GIIAPLA2) in tears of patients with atopic blepharoconjunctivitis (ABC), and to compare it with the GIIAPLA2 conce ntration of tears in age-mat...Background: To determine the concentration of group IIA phospholipase A2 (GIIAPLA2) in tears of patients with atopic blepharoconjunctivitis (ABC), and to compare it with the GIIAPLA2 conce ntration of tears in age-matched healthy controls. Methods: The diagnosis of AB C was confirmed with a positive skin prick test and the presence of atopic derma titis in lids. Conjunctival brush cytology was taken, and the cells including eo sinophils, neutrophils, lymphocytes, squamous epithelial cells, columnar epithel ial cells, metaplastic changes and the goblet cells were calculated separately. The GIIAPLA2 concentration of tears was measured with a time-resolved fluoroimm unoassay in 29 patients with ABC (mean age 36.3±12.7 years) and 29 normal subje cts (mean age 37.0±12.0 years). Results: The GIIAPLA2 concentration of tears in patients with ABC was 43.8±33.0 μg/ml, and in normal subjects it was 67.1±23 .3 μg/ml. The difference was statistically significant (P=0.001 8). The concent ration of GIIAPLA2 of tears was lowest in the subgroup of patients with ABC and dry eye (25.8 ±23.6 μg/ml), whereas it was only slightly decreased in patients with ABC and normal tear secretion (56.6±33.3 μg/ml). The difference between these two subgroups was statistically significant (P=0.011). There was no statis tically significant correlation between the GIIAPLA2 concentration of tears and the quantity of different conjunctival cells gathered by the brush cytology. How ever, an almost significant correlation was found between the GIIAPLA2 concentra tion in tears and conjunctival eosinophils. Conclusions: The results indicate th at in patients with ABC the GIIAPLA2 content of tears was decreased, without any dependence on the quantity of different conjunctival cells.展开更多
Purpose: To determine the concentration of group IIA phospholipase A2 (GIIAPLA 2) in tears of patients with ocular rosacea, and to compare it with GIIAPLA2 con centration in tears of age-matched healthy controls. Meth...Purpose: To determine the concentration of group IIA phospholipase A2 (GIIAPLA 2) in tears of patients with ocular rosacea, and to compare it with GIIAPLA2 con centration in tears of age-matched healthy controls. Methods: The GIIAPLA2 conc entration in tears was measured with a time-resolved fluoroimmunoassay in 21 pa tients with ocular rosacea (mean age 55.6 ±9.2 years) and in 21 normal subjects (mean age 53.4 ±8.2 years). Conjunctival brush cytologywas carried out and eos inophils, neutrophils, lymphocytes, squamous epithelial cells, columnar epitheli al cells, metaplastic changes and goblet cells were calculated separately. Resul ts: The GIIAPLA2 concentration in tears was statistically significantly lower in patients with ocular rosacea(31.0±18.4 μg/ml, p=0.0099) and, more spe- cifically, in patients who had dry eye (25.8±15.1 μg/ml, p=0.0034), compared to that in normal controls. There was no correlation between the GIIAPLA2 conte nt of tears and the conjunctival cells collected by the brush cytology. Conclusi on: The tears of patients with dry eye symptoms due to ocular rosacea have decre ased GIIAPLA2 content. The pathogenic importance of this finding is discussed.展开更多
文摘Background: To determine the concentration of group IIA phospholipase A2 (GIIAPLA2) in tears of patients with atopic blepharoconjunctivitis (ABC), and to compare it with the GIIAPLA2 conce ntration of tears in age-matched healthy controls. Methods: The diagnosis of AB C was confirmed with a positive skin prick test and the presence of atopic derma titis in lids. Conjunctival brush cytology was taken, and the cells including eo sinophils, neutrophils, lymphocytes, squamous epithelial cells, columnar epithel ial cells, metaplastic changes and the goblet cells were calculated separately. The GIIAPLA2 concentration of tears was measured with a time-resolved fluoroimm unoassay in 29 patients with ABC (mean age 36.3±12.7 years) and 29 normal subje cts (mean age 37.0±12.0 years). Results: The GIIAPLA2 concentration of tears in patients with ABC was 43.8±33.0 μg/ml, and in normal subjects it was 67.1±23 .3 μg/ml. The difference was statistically significant (P=0.001 8). The concent ration of GIIAPLA2 of tears was lowest in the subgroup of patients with ABC and dry eye (25.8 ±23.6 μg/ml), whereas it was only slightly decreased in patients with ABC and normal tear secretion (56.6±33.3 μg/ml). The difference between these two subgroups was statistically significant (P=0.011). There was no statis tically significant correlation between the GIIAPLA2 concentration of tears and the quantity of different conjunctival cells gathered by the brush cytology. How ever, an almost significant correlation was found between the GIIAPLA2 concentra tion in tears and conjunctival eosinophils. Conclusions: The results indicate th at in patients with ABC the GIIAPLA2 content of tears was decreased, without any dependence on the quantity of different conjunctival cells.
文摘Purpose: To determine the concentration of group IIA phospholipase A2 (GIIAPLA 2) in tears of patients with ocular rosacea, and to compare it with GIIAPLA2 con centration in tears of age-matched healthy controls. Methods: The GIIAPLA2 conc entration in tears was measured with a time-resolved fluoroimmunoassay in 21 pa tients with ocular rosacea (mean age 55.6 ±9.2 years) and in 21 normal subjects (mean age 53.4 ±8.2 years). Conjunctival brush cytologywas carried out and eos inophils, neutrophils, lymphocytes, squamous epithelial cells, columnar epitheli al cells, metaplastic changes and goblet cells were calculated separately. Resul ts: The GIIAPLA2 concentration in tears was statistically significantly lower in patients with ocular rosacea(31.0±18.4 μg/ml, p=0.0099) and, more spe- cifically, in patients who had dry eye (25.8±15.1 μg/ml, p=0.0034), compared to that in normal controls. There was no correlation between the GIIAPLA2 conte nt of tears and the conjunctival cells collected by the brush cytology. Conclusi on: The tears of patients with dry eye symptoms due to ocular rosacea have decre ased GIIAPLA2 content. The pathogenic importance of this finding is discussed.
