Smad8 is involvement in follicular development via the regulation of granulosa cell growth and steroidogenesis in mice

Background:SMAD family proteins(SMADs)are crucial transcription factors downstream of transforming growth factor beta(TGF-ß)/SMAD signaling pathways that have been reported to play a pivotal role in mammalian reproduction.However,the role of SMAD family member 8(SMAD8,also known as SMAD9),a member of the SMAD family,in mammalian reproduction remains unclear.Methods:We employed RNA interference techniques to knock down Smad8 expression in mouse granulosa cells(GCs)to investigate the effects of Smad8 on GC growth and steroidogenesis.Results:Our findings revealed a significant decrease in the proliferative capacity and a substantial increase in the apoptosis rate of GCs after transfection with Smad8-siRNA for 48 h.Subsequent hormone assays demonstrated a significant decrease in estradiol(E2)levels,whereas progesterone(P4)remained unchanged.Further mechanistic analysis showed that the mRNA expression of proliferating cell nuclear antigen(Pcna),Cyclin D2,cell cycle-dependent kinase 4(Cdk4),B-cell lymphoma-2(Bcl-2),estrogen receptor(Er),luteinizing hormone receptor(Lhr)and cytochrome P450 family 19 subfamily A member 1(Cyp19a1)significantly decreased.Conversely,the mRNA of cysteine aspartate proteinase 3(Caspase 3)significantly increased,wheras Bcl2-associated X(Bax),folliclestimulating hormone receptor(Fshr)and cytochrome P450 family 11 subfamily A member 1(Cyp11a1)remained unchanged compared to the controls.Conclusion:This study indicates that Smad8 knockdown inhibits cell proliferation,promotes apoptosis,reduces Er and Lhr transcription,and decreases E2 production in mouse GCs.These findings suggest that Smad8 may serve as a novel genetic marker for mammalian reproduction.

Machine learning with active pharmaceutical ingredient/polymer interaction mechanism:Prediction for complex phase behaviors of pharmaceuticals and formulations

The high throughput prediction of the thermodynamic phase behavior of active pharmaceutical ingredients(APIs)with pharmaceutically relevant excipients remains a major scientific challenge in the screening of pharmaceutical formulations.In this work,a developed machine-learning model efficiently predicts the solubility of APIs in polymers by learning the phase equilibrium principle and using a few molecular descriptors.Under the few-shot learning framework,thermodynamic theory(perturbed-chain statistical associating fluid theory)was used for data augmentation,and computational chemistry was applied for molecular descriptors'screening.The results showed that the developed machine-learning model can predict the API-polymer phase diagram accurately,broaden the solubility data of APIs in polymers,and reproduce the relationship between API solubility and the interaction mechanisms between API and polymer successfully,which provided efficient guidance for the development of pharmaceutical formulations.

A novel interfacial thermodynamic model for predicting solubility of nanoparticles coated by stabilizers

To improve the stability of nanoparticles in aqueous solution,polymer or surfactant,etc.are often added in solutions during the preparation process of nanoparticles,which can induce new interfaces that influence the solubility of nanoparticles.In this work,a novel interfacial thermodynamic model for describing the Gibbs energy of the nanoparticles coated by stabilizers was proposed to predict the solubility of nanoparticles.Within the developed model,the activity coefficient of nano metal system was determined by Davies model and that of nano drug system by Perturbed-Chain Statistical Associating Fluid Theory(PC-SAFT).The Gibbs energy of the interface was established as a function of molecular parameters via the application for nano metal system.Furthermore,the model was further used to predict the solubility of nano drugs itraconazole,fenofibrate,and griseofulvin.It was found that the Gibbs energy of the interface plays an important role especially when the radius of nano metal is less than 40 nm,and the developed model can predict the solubility of nano drug with high accuracy in comparison with the experimental data as well as predict the changing trend of solubility of nano drugs that increases as the particle size decreases.Meanwhile,the stabilization mechanism of stabilizers on nano drugs was studied which provided theoretical guidance for the selection of polymer or surfactant stabilizer.These findings showed that the developed model can provide a reliable prediction of the solubility of nanoparticles and help to comprehend the stabilization mechanism of the stabilizers on nano drugs with different particle sizes,which is expected to provide important information for the design of nano drugs formulations.

Determination of Residual Amount of 15 Plant Growth Regulators in Bean Sprouts by HPLC-MS/MS

[Objectives]This study was conducted to effectively monitor PGR residues in bean sprouts to provide guarantee for the food safety of agricultural products.[Methods]A high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the determination of residues of 15 plant growth regulators(PGRs)in bean sprouts was established using bean sprouts as an experimental material.Samples were extracted with a solution containing 5%acetic acid-acetonitrile(1∶99,V/V),purified with anhydrous magnesium sulfate,and diluted with methanol solvent to constant volume.The solutions were filtered through 0.22μm filtering membrane and the target analytes were separated on a Phenomenex H18 column.The identification of each compound was established by retention time matching along with the accurate mass measurement of the precursor ions and their main fragment ions.The quantification was carried out using matrix-matched external standard method.[Results]The retention time of the 15 PGRs were found in the range from 5.8-11.7 min under the optimized conditions.The linear relation was good in the concentration range of 0.005-0.050μg/ml,and the correlation coefficients of the 15 PGRs were≥0.9990.The limits of detection were in the range of 0.03-0.92 g/kg,and the limits of quantification were in the range of 0.50-2.10μg/kg.The average recovery in the recovery test at 3 concentration levels was 80%-110%,and the relative standard deviations were in the range of 2.8%-7.5%.[Conclusions]This method is simple and accurate,and can quickly qualitatively and quantitatively analyze the residues of 15 PGRs in bean sprouts.The proposed procedure was simple,quick and accurate for the simultaneous determination of the 15 PGRs in bean sprout.

新时期民族高校图书馆文献资源建设现状探析——以呼和浩特民族学院为例

通过调查分析学生、教师、专家及相关处系人员的图书文献需要数据,找出了呼和浩特民族学院图书馆文献资源存在的问题,探讨了文献建设应注意的因素,提出了建设性措施,以期促进图书馆更好地发挥职能,提供服务。

Comparison and Analysis of the Major Ingredients of Different Chinese Chestnut Varieties

[Objectives]This study was conducted to investigate the differences of main nutrient components in 29 different varieties of Chinese chestnut.[Methods]The protein contents,starch contents,Vc contents,soluble sugar contents and inorganic element contents in the 29 different varieties of Chinese chestnut in the north were detected and compared.[Results]The main nutrient components in the 29 varieties of Chinese chestnut were as follows:amylose content 47.16%-63.27%,protein content 3.23%-7.58%,soluble sugar content 7.23%-16.17%,Vc content 2.42-9.42 mg/100 g,Fe content 7.20-17.28 mg/kg,K content 3611-8940 mg/kg,Zn content 4.43-9.24 mg/kg,Ca content 120-304 mg/kg,and P content 752-1658 mg/kg.In general,four varieties,"Dabanhong","Yanbao","Duanzhi"and"Jinhua",have higher nutritional components.[Conclusions]This study provides a theoretical basis for the processing,utilization,storage,purchase,breeding and scientific management of Chinese chestnut in the future.

Research Progression on Isolation and Purification of Resveratrol

Resveratrol is a ployphenolic compound which has a wide variety of pharmacological activity. This paper reviewed the physicochemical properties of resveratrol and its purification technology including macroporous resin method, high-speed countercurrent chromatography method, molecular imprinting method, membrane separation method, column chromatography method, two-aqueous phase extraction method and the combination of purification methods, so as to provide certain reference for further research and development.

陈春燕:“新一代刘三姐”为时代放歌

“刘三姐”的民歌尚过岁月的河,刻进了几代人的记忆。陈春燕为人民创作、为时代放歌,被誉为“新一代刘三姐”陈春燕是瑶族歌唱家、国家一级演员、广西壮族自治区南宁市群众艺术馆馆长。《飘飘瑶》《壮乡春早》《漓江情》,壮乡的民族文化之美,在她的歌声中飞向世界。她带领志愿服务团队文化下乡,开展喜闻乐见的活动,让艺术之花在泥土的芬芳中生长、在群众中绽放。

姜丽娟:在“梦开始的地方”梦想成真

“习近平总书记强调,妇女事业和妇联工作要始终坚持正确政治方向,与党同心同行。我在工作实践中发现,调动妇女的主观能动性十分重要。很多女性善于沟通、具有亲和力,在基层社会治理中发挥了重要作用。作为一名农村基层的妇女工作者,我要引领妇女坚定不移听党话、跟党走,在全面推进乡村振兴共同富裕中,聚‘她’力量、展‘她’活力。”浙江省杭州市淳安县枫树岭镇下姜村党总支书记、村委会主任、妇联主席,中国妇女十三大代表姜丽娟说。作为村里第一位返乡创业的大学生,姜丽娟的青春已经与家乡的这片土地、与带领村民致富的使命联系在了一起。

技能成就梦想

技能是强国之基、立业之本。习近平总书记指出:“要在全社会弘扬精益求精的工匠精神,激励广大青年走技能成才、技能报国之路。”党的二十大报告提出,加快建设国家战略人才力量,努力培养造就更多大师、战略科学家、一流科技领军人才和创新团队、青年科技人才、卓越工程师、大国工匠、高技能人才。

2023,祝福千家万户幸福安康

Let Family Be Important Basis forNational Development,Ethnic Progress,Social Harmony让家庭成为国家发展、民族进步、社会和谐的重要基点 Since the 18th National Congress of the Communist Party of China(CPC),held in 2012,the CPC Central Committee with Comrade Xi Jinping at the core has attached great importance to fostering stronger family ties,values and traditions.Families have become an important basis for national development,ethnic progress and social harmony.Women have continued to play a unique role in fostering stronger family ties,values and traditions,and women have become builders and advocates of the happiness and well-being of families.

内蒙古大青山自然保护区生态服务价值评估与建设

内蒙古大青山地处蒙古高原中温型草原和暖温性草原交汇处,是阴山山地中段保存最好的次生林区,气候、土壤、地质地貌、动物、植物都极具代表性。同时,大青山是黄河中上游的重要水源地,具有防止水土流失、改善水文状况、净化水质等作用,是阴山山地重要的水源涵养地之一。为客观地反映大青山森林生态系统在经济社会可持续发展中的地位、贡献与作用,提高全民森林生态保护意识,并为大青山森林资源经营利用、管理及生态环境保护提供科学依据,对大青山森林生态功能及其价值进行评估,促使其发挥更大的生态、经济和社会效益。依据2016年国家林业局《森林生态服务功能评估规范》(LY/T1721-2008),本文以内蒙古大青山自然保护区为主要研究评价对象,并结合森林净化天然大气、涵养天然水源等6种应用类型8项评价指标结果对森林资源生态系统管理服务应用价值进行线性定量分析评价。

Experiencing Charm of Peking Opera While Cycling in Beijing

Beijing Overseas Cultural Exchanges Center has created an online roadmap of the Cultural Tour of Peking Opera,which highlights Peking Opera theaters,former residences of Peking Opera artists,guild halls and other places used for Peking Opera performances.The center advocates low-carbon travel,and it recommends a cycling route of the Cultural Tour of Peking Opera.While cycling along the streets of Beijing,people can visit landmark buildings of the Peking Opera,and they can feel the unique charm of the city.

Women Probe Beauty of Science,Technology

Women have become an important force in promoting scientific and technological advancement globally.Many women scientists,worldwide,have created“miracles”that have greatly promoted the development of human civilization.

WOMEN RETURNEES CREATE WONDERFUL LIVES IN HOME COUNTRY

China has promulgated preferential policies in recent years to encourage overseas Chinese to return home,to realize the value of their lives.As a result,more and more young Chinese have returned to China,after they have completed their studies in other countries,so they tan pursue their dreams and make contributions to their home country.Women of China recently interviewed several returned overseas Chinese women,who shared their stories of devoting their lives to building their motherland into a better place.