[ Objective] To understand the physicochemical properties of musk deer pneumonia and purulent disease viruses. [ Method] The pneu- monia and purulent disease viruses were isolated from the abnormal and purulent lung t...[ Objective] To understand the physicochemical properties of musk deer pneumonia and purulent disease viruses. [ Method] The pneu- monia and purulent disease viruses were isolated from the abnormal and purulent lung tissues of musk deer. Then the isolated viruses were inocula- ted into the Vero cells. After culturing, the virus solution was collected and used to determine TCID50 and genoma types. The sensitivity to fat sol- vent, resistance to hydrochloric acid and trypsin as well as tolerance to heat of the musk deer pneumonia and purulent disease viruses were detec- ted, respectively. [ Result] The obvious cytopathic effects （CPE） were found in Veto cells infected by the isolated viruses. The virus was 2-1.43 TCID50/ml and its genome was RNA. The virus was not sensitive to chloroform, 1% trypsin and heats, and it had a certain tolerance to 0.1 mol/L hy- drochloric acid. [ Conclusion] The study on the physicochemical properties of musk deer pneumonia and purulent disease viruses lays a foundation for prevention and control of the musk deer pneumonia and purulent diseases.展开更多
文摘目的研究麦芽三糖酶对大米淀粉回生性质的影响。方法以不同麦芽三糖酶添加量(0、200、300、400、500、600 U)水解大米淀粉,利用差示扫描量热仪、X射线衍射仪测定酶解产物的回生性质和重结晶性质,并利用凝胶色谱分析系统分析酶对淀粉分子结构的影响。结果麦芽三糖酶酶解大米淀粉,随着水解程度增加可显著降低其4℃储藏14 d后的回生焓、相对结晶度以及分子尺寸,显著提高脱支大米淀粉酶解产物中聚合度(degree of polymerization,DP)<9的极短链所占比例;淀粉水解率≥25.96%后,实验条件下未能检测到回生焓和重结晶峰。结论麦芽三糖酶酶解大米淀粉后可有效抑制酶解产物的回生,当淀粉水解率≥25.96%,大米淀粉酶解产物的回生被完全抑制,这主要是因为支链淀粉的外链被水解生成具有大量DP<9极短链的分支糊精。本研究可为麦芽三糖酶应用大米淀粉制品的酶法回生控制提供理论参考和技术依据。
基金supported by the Youth Fund Projects of Sichuan Education Department(07ZB060)Technology Support Projects of Sichuan Science and Technology Department(2009SZ0228)
文摘[ Objective] To understand the physicochemical properties of musk deer pneumonia and purulent disease viruses. [ Method] The pneu- monia and purulent disease viruses were isolated from the abnormal and purulent lung tissues of musk deer. Then the isolated viruses were inocula- ted into the Vero cells. After culturing, the virus solution was collected and used to determine TCID50 and genoma types. The sensitivity to fat sol- vent, resistance to hydrochloric acid and trypsin as well as tolerance to heat of the musk deer pneumonia and purulent disease viruses were detec- ted, respectively. [ Result] The obvious cytopathic effects （CPE） were found in Veto cells infected by the isolated viruses. The virus was 2-1.43 TCID50/ml and its genome was RNA. The virus was not sensitive to chloroform, 1% trypsin and heats, and it had a certain tolerance to 0.1 mol/L hy- drochloric acid. [ Conclusion] The study on the physicochemical properties of musk deer pneumonia and purulent disease viruses lays a foundation for prevention and control of the musk deer pneumonia and purulent diseases.
