重构色彩空间下陈旧印章印文自适应Canny检测

图像内陈旧印章印文边缘检测过程中,经过历时性变化的印章印文因表面氧化变色,背景颜色复杂且杂点较多易导致边缘误检。针对上述问题,提出一种重构色彩空间下自适应Canny算子的陈旧印章印文边缘检测方法。首先,将陈旧印章印文图像的RGB色彩分量经线性变换至定义的SN色彩空间,根据SN色彩空间内颜色分量分离出陈旧印章印文主体;然后,使用基于双边滤波的自适应Canny算子对形态学处理后图像进行边缘检测,其中Canny算子的阈值由陈旧印章印文主体的均值、方差自适应计算。实验结果表明,重构色彩空间下提取陈旧印章印文主体色彩分量效果更好、Calinski-Harabasz值更优,陈旧印章印文边缘检测中峰值信噪比、均方差等多个指标较传统边缘检测算法更优,且能够更好地抑制伪边缘出现。

HPLC双波长法测定米力农注射液有关物质

目的建立高效液相色谱法(HPLC)测定米力农注射液有关物质,有效控制不同生产企业的产品质量。方法采用Shiseido C18(250 mm×4.6 mm,5μm)色谱柱,柱温30℃,流动相为水-甲醇-硼酸钠缓冲液(725:250:25),流速1.0 ml/min,检测波长为284 nm(5-羟甲基糠醛)和254 nm(米力农杂质I、米力农杂质Ⅱ、其他杂质)。结果米力农与各杂质峰均能有效分离,米力农、5-羟甲基糠醛、米力农杂质I、米力农杂质Ⅱ分别在0.2~5μg/ml(r=0.9999)、2~50μg/ml(r=0.9996)、0.2~5μg/ml(r=1.0000)、0.2~5μg/ml(r=0.9999)浓度范围内线性关系良好,5-羟甲基糠醛、米力农杂质I、米力农杂质Ⅱ的回收率分别为99.9%(RSD=0.49%,n=9)、102.0%(RSD=0.48%,n=9)和95.8%(RSD=1.47%,n=9);定量限分别为5 ng/ml,1ng/ml,0.025μg/ml,0.05μg/ml。结论建立的方法灵敏准确,可有效地对米力农注射液的有关物质进行定量测定。

Scapaundulin C,a novel labdane diterpenoid isolated from Chinese liverwort Scapania undulate,inhibits acetylcholinesterase activity

In the present study, scapaundulin C(1), a new labdane diterpenoid, and four related known compounds scapaundulin A(2), 5?, 8?, 9?-trihydroxy-13E-labden-12-one(3), 5?, 8?-dihydroxy-13E-labden-12-one(4), and(13S)-15-hydroxylabd-8(17)-en-19-oic acid(5), were isolated from the Chinese liverwort Scapania undulate(L.) Dum., using column chromatography. The structures of these compounds were determined on the basis of 1D- and 2D-NMR analyses. The acetylcholinesterase(Ach E) inhibitory activity was evaluated using a bioautographic TLC assay and the cytotoxic activity was evaluated by the MTT method. All the compounds were reported for the first time to exhibit moderate Ach E inhibitory activity with minimal inhibitory quantities ranging from 250 to 500 ng. All the compounds were tested for their cytotoxicity against five human tumor cell lines, A549, K562, A2780, Hela, and HT29, and compounds 3 and 4 exhibited moderate inhibitory effects on the growth of A2780 cells.