Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics; however, little information on these interactions has been reported. In this study, th...Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics; however, little information on these interactions has been reported. In this study, the bacterial communities associated with five strains of Microcystis aeruginosa, three species of other Microcystis spp., and four representative species of non-Microcystis cyanobacteria were compared. Bacterial 16S rDNA fragments were amplified and separated by denaturing gradient gel electrophoresis （DGGE） followed by DNA sequence analysis. The similarities among bacterial communities associated with these cyanobacteria were compared to the digitized DGGE profiles using the cluster analyses. The bacterial community structure of all cyanobacterial cultures differed. Cluster analysis showed that the similarity values among M. aeruginosa cultures were higher than those of other cyanobacterial cultures. Sequence analysis of DGGE fragments indicated the presence of bacteria including, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria in the cyanobacterial cultures. Members of the Sphingomonadales were the prevalent group among the Microcystis-associated bacteria. The results provided further evidence for species-specific associations between cyanoabcteria and heterotrophic bacteria, which are useful for understanding interactions between Microcystis and their associated bacteria.展开更多
目的观察比伐芦定对Apo E基因敲除小鼠动脉粥样硬化的影响。方法将20只Apo E-/-小鼠随机分成模型组和治疗组,10只C57BL/6J小鼠作为空白对照组。模型组和治疗组给予高脂饲料喂养,空白组给予普通饲料喂养。于第9周开始,治疗组给予比伐芦...目的观察比伐芦定对Apo E基因敲除小鼠动脉粥样硬化的影响。方法将20只Apo E-/-小鼠随机分成模型组和治疗组,10只C57BL/6J小鼠作为空白对照组。模型组和治疗组给予高脂饲料喂养,空白组给予普通饲料喂养。于第9周开始,治疗组给予比伐芦定治疗4周。全自动生化分析仪检测血清中TG、TC、HDL-C、LDL-C含量,Elisa法检测各组小鼠血清TN F-α、IL-6水平变化,R T-PCR法检测主动脉VEGF、MMP-9、HIF-1αm R N A表达水平。结果与空白对照组比较,模型组小鼠血清TC、TG、LDL-C、TNF-α、IL-6水平显著升高(P<0.01),HDL水平显著降低(P<0.01),VEGF、MMP-9、HIF1-αm R N A表达水平显著升高(P<0.01);与模型组比较,治疗组小鼠血清水平、炎症因子水平以及VEGF、MMP-9、HIF1-αm R N A表达水平明显改善(P<0.01)。结论中药水蛭的有效成分水蛭素的人工合成药物比伐芦定能够通过改善血脂水平,血清炎症因子水平及血管新生等方面发挥良好的抗动脉粥样硬化作用。展开更多
基金supported by the National Basic Research Program (973) of China (No. 2008CB418004)the Natural Science Foundation of Jiangsu Province (No.BK2007150)the China Postdoctoral Science Foundation (No. 20070410345)
文摘Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics; however, little information on these interactions has been reported. In this study, the bacterial communities associated with five strains of Microcystis aeruginosa, three species of other Microcystis spp., and four representative species of non-Microcystis cyanobacteria were compared. Bacterial 16S rDNA fragments were amplified and separated by denaturing gradient gel electrophoresis （DGGE） followed by DNA sequence analysis. The similarities among bacterial communities associated with these cyanobacteria were compared to the digitized DGGE profiles using the cluster analyses. The bacterial community structure of all cyanobacterial cultures differed. Cluster analysis showed that the similarity values among M. aeruginosa cultures were higher than those of other cyanobacterial cultures. Sequence analysis of DGGE fragments indicated the presence of bacteria including, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria in the cyanobacterial cultures. Members of the Sphingomonadales were the prevalent group among the Microcystis-associated bacteria. The results provided further evidence for species-specific associations between cyanoabcteria and heterotrophic bacteria, which are useful for understanding interactions between Microcystis and their associated bacteria.
文摘目的观察比伐芦定对Apo E基因敲除小鼠动脉粥样硬化的影响。方法将20只Apo E-/-小鼠随机分成模型组和治疗组,10只C57BL/6J小鼠作为空白对照组。模型组和治疗组给予高脂饲料喂养,空白组给予普通饲料喂养。于第9周开始,治疗组给予比伐芦定治疗4周。全自动生化分析仪检测血清中TG、TC、HDL-C、LDL-C含量,Elisa法检测各组小鼠血清TN F-α、IL-6水平变化,R T-PCR法检测主动脉VEGF、MMP-9、HIF-1αm R N A表达水平。结果与空白对照组比较,模型组小鼠血清TC、TG、LDL-C、TNF-α、IL-6水平显著升高(P<0.01),HDL水平显著降低(P<0.01),VEGF、MMP-9、HIF1-αm R N A表达水平显著升高(P<0.01);与模型组比较,治疗组小鼠血清水平、炎症因子水平以及VEGF、MMP-9、HIF1-αm R N A表达水平明显改善(P<0.01)。结论中药水蛭的有效成分水蛭素的人工合成药物比伐芦定能够通过改善血脂水平,血清炎症因子水平及血管新生等方面发挥良好的抗动脉粥样硬化作用。
