目的探讨早期腹腔镜胆囊切除术(laparoscopic cholecystectomy,LC)治疗急诊普通外科(emergency general surgery,EGS)Ⅲ级及以上重症急性胆囊炎的安全性及有效性。方法回顾分析2017年1月至2022年6月于华中科技大学同济医学院附属武汉市...目的探讨早期腹腔镜胆囊切除术(laparoscopic cholecystectomy,LC)治疗急诊普通外科(emergency general surgery,EGS)Ⅲ级及以上重症急性胆囊炎的安全性及有效性。方法回顾分析2017年1月至2022年6月于华中科技大学同济医学院附属武汉市中心医院接受LC的急性胆囊炎病人1381例,根据病情严重程度不同分为重症组(112例)和普通组(1269例)。分析比较两组术前一般资料、手术时间、术中失血量、中转开腹率、术后转ICU率、围手术期死亡率、术后引流量、术后住院时间及并发症发生率等。结果重症组相较于普通组,手术时间[(100.54±22.23)min比(61.31±10.48)min]、术中失血量[40.00(20.00,60.00)mL比5.00(5.00,10.00)mL]、术后引流量[100.00(60.00,152.50)mL比30.00(20.00,40.00)mL]、术后住院时间[(7.31±2.68)d比(4.03±1.23)d]均有所增多,差异具有统计学意义(P<0.05);重症组中转开腹率为3.57%(P=0.208)、术后转ICU率为4.46%(P=0.297)、围手术期死亡率为0、并发症发生率为7.14%(P=0.133),与普通组相比差异无统计学意义。结论EGSⅢ级及以上重症急性胆囊炎早期行LC治疗,术后并发症发生率等和轻度急性胆囊炎术后相比并不增加,是相对安全、有效的,可作为该类重症急性胆囊炎的一种治疗选择。展开更多
Although 18 S r DNA sequence is extremely conservative, the polymorphism still has been found in few species.In the present study, three types(Type A, B and C) of 18 S r DNA sequence coexisted in Cynoglossus lineolatu...Although 18 S r DNA sequence is extremely conservative, the polymorphism still has been found in few species.In the present study, three types(Type A, B and C) of 18 S r DNA sequence coexisted in Cynoglossus lineolatus genome, suggesting a non-concerted evolution process, rather than a strictly concerted evolution fashion.Based on the differences of sequence variation, GC content, secondary structure and minimum free energy,Types A and B were speculated as the potential pseudogenes. Additionally, a fascinating finding was a 189-bp duplication of 18 S r DNA in Type A sequence. To our knowledge, this is the first report on such a long duplication in teleostean ribosomal DNA. Compared with several theories accounting for the formation of tandem repeats, the unequal crossing over model was thought to be the most likely mechanism to generate the189-bp duplication of 18 S r DNA. These results not only provide a novel molecular evidence for the unequal crossing over model, but also benefit for the further study on 18 S r DNA in fishes.展开更多
Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chla...Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.展开更多
文摘目的探讨早期腹腔镜胆囊切除术(laparoscopic cholecystectomy,LC)治疗急诊普通外科(emergency general surgery,EGS)Ⅲ级及以上重症急性胆囊炎的安全性及有效性。方法回顾分析2017年1月至2022年6月于华中科技大学同济医学院附属武汉市中心医院接受LC的急性胆囊炎病人1381例,根据病情严重程度不同分为重症组(112例)和普通组(1269例)。分析比较两组术前一般资料、手术时间、术中失血量、中转开腹率、术后转ICU率、围手术期死亡率、术后引流量、术后住院时间及并发症发生率等。结果重症组相较于普通组,手术时间[(100.54±22.23)min比(61.31±10.48)min]、术中失血量[40.00(20.00,60.00)mL比5.00(5.00,10.00)mL]、术后引流量[100.00(60.00,152.50)mL比30.00(20.00,40.00)mL]、术后住院时间[(7.31±2.68)d比(4.03±1.23)d]均有所增多,差异具有统计学意义(P<0.05);重症组中转开腹率为3.57%(P=0.208)、术后转ICU率为4.46%(P=0.297)、围手术期死亡率为0、并发症发生率为7.14%(P=0.133),与普通组相比差异无统计学意义。结论EGSⅢ级及以上重症急性胆囊炎早期行LC治疗,术后并发症发生率等和轻度急性胆囊炎术后相比并不增加,是相对安全、有效的,可作为该类重症急性胆囊炎的一种治疗选择。
基金The National Natural Science Foundation of China under contract No.31272273the Open Research Fund Program of Guangxi Key Laboratory of Mangrove Conservation and Utilization under contract No.GKLMC-201502
文摘Although 18 S r DNA sequence is extremely conservative, the polymorphism still has been found in few species.In the present study, three types(Type A, B and C) of 18 S r DNA sequence coexisted in Cynoglossus lineolatus genome, suggesting a non-concerted evolution process, rather than a strictly concerted evolution fashion.Based on the differences of sequence variation, GC content, secondary structure and minimum free energy,Types A and B were speculated as the potential pseudogenes. Additionally, a fascinating finding was a 189-bp duplication of 18 S r DNA in Type A sequence. To our knowledge, this is the first report on such a long duplication in teleostean ribosomal DNA. Compared with several theories accounting for the formation of tandem repeats, the unequal crossing over model was thought to be the most likely mechanism to generate the189-bp duplication of 18 S r DNA. These results not only provide a novel molecular evidence for the unequal crossing over model, but also benefit for the further study on 18 S r DNA in fishes.
基金This work was financially supported by the"863"Project of China under contract No.2002AA626020the National Nalural Science Foundation of China under contract No.30570242.
文摘Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.
