Microorganisms regulate the responses of terrestrial ecosystems to anthropogenic nutrient inputs.The escalation of anthropogenic activities has resulted in a rise in the primary terrestrial constraining elements,namel...Microorganisms regulate the responses of terrestrial ecosystems to anthropogenic nutrient inputs.The escalation of anthropogenic activities has resulted in a rise in the primary terrestrial constraining elements,namely nitrogen(N)and phosphorus(P).Nevertheless,the specific mechanisms governing the influence of soil microbial community structure and ecological processes in ecologically vulnerable and delicate semi-arid loess agroecosystems remain inadequately understood.Therefore,we explored the effects of different N and P additions on soil microbial community structure and its associated ecological processes in the farmland of Chinese Loess Plateau based on a 36-a long-term experiment.Nine fertilization treatments with complete interactions of high,medium,and low N and P gradients were set up.Soil physical and chemical properties,along with the microbial community structure were measured in this study.Additionally,relevant ecological processes such as microbial biomass,respiration,N mineralization,and enzyme activity were quantified.To elucidate the relationships between these variables,we examined correlation-mediated processes using statistical techniques,including redundancy analysis(RDA)and structural equation modeling(SEM).The results showed that the addition of N alone had a detrimental effect on soil microbial biomass,mineralized N accumulation,andβ-1,4-glucosidase activity.Conversely,the addition of P exhibited an opposing effect,leading to positive influences on these soil parameters.The interactive addition of N and P significantly changed the microbial community structure,increasing microbial activity(microbial biomass and soil respiration),but decreasing the accumulation of mineralized N.Among them,N24P12 treatment showed the greatest increase in the soil nutrient content and respiration.N12P12 treatment increased the overall enzyme activity and total phospholipid fatty acid(PLFA)content by 70.93%.N and P nutrient contents of the soil dominate the microbial community structure and the corresponding changes in hydrolytic enzymes.Soil microbial biomass,respiration,and overall enzyme activity are driven by mineralized N.Our study provides a theoretical basis for exploring energy conversion processes of soil microbial community and environmental sustainability under long-term N and P additions in semi-arid loess areas.展开更多
【背景】磷转化微生物对于自然环境中的磷循环具有重要作用。此类微生物通过对环境中磷素的溶解矿化和吸收转运等作用促进资源高效利用,减少环境污染,对粮食安全和生态系统稳定也具有积极的影响。【目的】探究近年来国内外关于磷转化微...【背景】磷转化微生物对于自然环境中的磷循环具有重要作用。此类微生物通过对环境中磷素的溶解矿化和吸收转运等作用促进资源高效利用,减少环境污染,对粮食安全和生态系统稳定也具有积极的影响。【目的】探究近年来国内外关于磷转化微生物的发展热点及趋势,展示该领域的知识结构和知识演化过程,为后续研究提供可行性的参考和启示。【方法】应用CiteSpace可视化软件对2002−2022年发表在中国知网(CNKI)和Web of Science(WOS)数据库中的文献通过关键词共现分析、关键词聚类分析、关键词突现分析、发文数量分析、国家分布、作者合作共现及机构合作共现等方式对磷转化微生物的研究现状及新兴趋势进行分析。【结果】最终共筛选出887篇有效文献。磷转化微生物研究在2016年后开始蓬勃发展,近几年增速加快;中国和美国学者是此领域重要的研究力量;中国是发文量最多的国家,中国科学院是发文量最多的机构;热门的研究领域为菌株的分类与筛选、代谢途径、营养元素循环以及环境污染与生态保护。【结论】磷转化微生物具有广阔的发展前景。目前,生物炭及磷转化微生物的响应机制正在成为新的研究热点。此类技术在未来的广泛应用是必然的发展方向。本文以可视化的方式阐释了磷转化微生物研究发展态势,提出了未来重点研究方向,为农业生产与可持续发展提供了重要的理论基础。展开更多
基金funded by the Project of Science and Technology Department of Shaanxi Province,China(2022NY-074)the National Natural Science Foundation of China(41501255)+1 种基金the Xi'an Science and Technology Project(21NYYF0033)the Fundamental Research Funds for the Central Universities(SYJS202224,GK202206032).
文摘Microorganisms regulate the responses of terrestrial ecosystems to anthropogenic nutrient inputs.The escalation of anthropogenic activities has resulted in a rise in the primary terrestrial constraining elements,namely nitrogen(N)and phosphorus(P).Nevertheless,the specific mechanisms governing the influence of soil microbial community structure and ecological processes in ecologically vulnerable and delicate semi-arid loess agroecosystems remain inadequately understood.Therefore,we explored the effects of different N and P additions on soil microbial community structure and its associated ecological processes in the farmland of Chinese Loess Plateau based on a 36-a long-term experiment.Nine fertilization treatments with complete interactions of high,medium,and low N and P gradients were set up.Soil physical and chemical properties,along with the microbial community structure were measured in this study.Additionally,relevant ecological processes such as microbial biomass,respiration,N mineralization,and enzyme activity were quantified.To elucidate the relationships between these variables,we examined correlation-mediated processes using statistical techniques,including redundancy analysis(RDA)and structural equation modeling(SEM).The results showed that the addition of N alone had a detrimental effect on soil microbial biomass,mineralized N accumulation,andβ-1,4-glucosidase activity.Conversely,the addition of P exhibited an opposing effect,leading to positive influences on these soil parameters.The interactive addition of N and P significantly changed the microbial community structure,increasing microbial activity(microbial biomass and soil respiration),but decreasing the accumulation of mineralized N.Among them,N24P12 treatment showed the greatest increase in the soil nutrient content and respiration.N12P12 treatment increased the overall enzyme activity and total phospholipid fatty acid(PLFA)content by 70.93%.N and P nutrient contents of the soil dominate the microbial community structure and the corresponding changes in hydrolytic enzymes.Soil microbial biomass,respiration,and overall enzyme activity are driven by mineralized N.Our study provides a theoretical basis for exploring energy conversion processes of soil microbial community and environmental sustainability under long-term N and P additions in semi-arid loess areas.
文摘【背景】磷转化微生物对于自然环境中的磷循环具有重要作用。此类微生物通过对环境中磷素的溶解矿化和吸收转运等作用促进资源高效利用,减少环境污染,对粮食安全和生态系统稳定也具有积极的影响。【目的】探究近年来国内外关于磷转化微生物的发展热点及趋势,展示该领域的知识结构和知识演化过程,为后续研究提供可行性的参考和启示。【方法】应用CiteSpace可视化软件对2002−2022年发表在中国知网(CNKI)和Web of Science(WOS)数据库中的文献通过关键词共现分析、关键词聚类分析、关键词突现分析、发文数量分析、国家分布、作者合作共现及机构合作共现等方式对磷转化微生物的研究现状及新兴趋势进行分析。【结果】最终共筛选出887篇有效文献。磷转化微生物研究在2016年后开始蓬勃发展,近几年增速加快;中国和美国学者是此领域重要的研究力量;中国是发文量最多的国家,中国科学院是发文量最多的机构;热门的研究领域为菌株的分类与筛选、代谢途径、营养元素循环以及环境污染与生态保护。【结论】磷转化微生物具有广阔的发展前景。目前,生物炭及磷转化微生物的响应机制正在成为新的研究热点。此类技术在未来的广泛应用是必然的发展方向。本文以可视化的方式阐释了磷转化微生物研究发展态势,提出了未来重点研究方向,为农业生产与可持续发展提供了重要的理论基础。
