Objective:To study anxiolytic property of hydro alchohol extract and to estimate polyphenols present in the extract by HPLC.Methods:To evaluate anxiolytic properly two animal models were used viz.Elevated T maze and h...Objective:To study anxiolytic property of hydro alchohol extract and to estimate polyphenols present in the extract by HPLC.Methods:To evaluate anxiolytic properly two animal models were used viz.Elevated T maze and hyponeophagia.Diazepam(1 mg/kg body wt.) served as the standard anxiolvtic agent for all the tests.The dried extract of the plant leaf in doses of 100.200 and 400 mg/kg body weight was administered orally to mice for duration of 1? or 30 days and locomotor and anxiolytic activities were performed.Polyphenols was estimated using HPLC. Results:The HPLC analysis of the polyphenols revealed the presence chlorogenic acid,vanillin, epicatechin,caffeic acid,rutin hydrate,sinapic acid,quercetin-3-rhamnoside.p-coumeric acid and quercitin.Time spent and number of entries into the open arm was improved in 30 days treated animals than that of 15 days treated groups,200 and 400 mg/kg body weight treated group showed significant results when comparing with the control group.Conclusions:Te hydro alcohol extract rich in Polyphenols and other secondary metabolites is a potent anxiolytic agent.展开更多
文摘Objective:To study anxiolytic property of hydro alchohol extract and to estimate polyphenols present in the extract by HPLC.Methods:To evaluate anxiolytic properly two animal models were used viz.Elevated T maze and hyponeophagia.Diazepam(1 mg/kg body wt.) served as the standard anxiolvtic agent for all the tests.The dried extract of the plant leaf in doses of 100.200 and 400 mg/kg body weight was administered orally to mice for duration of 1? or 30 days and locomotor and anxiolytic activities were performed.Polyphenols was estimated using HPLC. Results:The HPLC analysis of the polyphenols revealed the presence chlorogenic acid,vanillin, epicatechin,caffeic acid,rutin hydrate,sinapic acid,quercetin-3-rhamnoside.p-coumeric acid and quercitin.Time spent and number of entries into the open arm was improved in 30 days treated animals than that of 15 days treated groups,200 and 400 mg/kg body weight treated group showed significant results when comparing with the control group.Conclusions:Te hydro alcohol extract rich in Polyphenols and other secondary metabolites is a potent anxiolytic agent.
