Background:Fatness traits in animals are important for their growth,meat quality,reproductive performance,and immunity.The liver is the principal organ of the regulation of lipid metabolism,and this study used massive...Background:Fatness traits in animals are important for their growth,meat quality,reproductive performance,and immunity.The liver is the principal organ of the regulation of lipid metabolism,and this study used massive parallelized high-throughput sequencing technologies to determine the porcine liver tissue transcriptome architecture of two full-sibling Songliao black pigs harboring extremely different phenotypes of backfat thickness.Results:The total number of reads produced for each sample was in the region of 53 million,and 8,226 novel transcripts were detected.Approximately 92 genes were differentially regulated in the liver tissue,while 31 spliced transcripts and 33 primary transcripts showed significantly differential expression between pigs with higher and lower backfat thickness.Genes that were differentially expressed were involved in the metabolism of various substances,small molecule biochemistry,and molecular transport.Conclusions:Genes involved in the regulation of lipids could play an important role in lipid and fatty acid metabolism in the liver.These results could help us understand how liver metabolism affects the backfat thickness of pigs.展开更多
Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting...Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting consumers’choice of pork.Weighted gene co-expression network analysis(WGCNA)is effective in pig genetic studies.Therefore,this study aimed to identify modules that co-express genes associated with fat deposition in pigs(Songliao black and Landrace breeds)with extreme levels of backfat(high and low)and to identify the core genes in each of these modules.Results:We used RNA sequences generated in different pig tissues to construct a gene expression matrix consisting of 12,862 genes from 36 samples.Eleven co-expression modules were identified using WGCNA and the number of genes in these modules ranged from 39 to 3,363.Four co-expression modules were significantly correlated with backfat thickness.A total of 16 genes(RAD9A,IGF2R,SCAP,TCAP,SMYD1,PFKM,DGAT1,GPS2,IGF1,MAPK8,FABP,FABP5,LEPR,UCP3,APOF,and FASN)were associated with fat deposition.Conclusions:RAD9A,TCAP,SMYD1,PFKM,GPS2,and APOF were the key genes in the four modules based on the degree of gene connectivity.Combining these results with those from differential gene analysis,SMYD1 and PFKM were proposed as strong candidate genes for body size traits.This study explored the key genes that regulate porcine fat deposition and lays the foundation for further research into the molecular regulatory mechanisms underlying porcine fat deposition.展开更多
Background:Natural astaxanthin(ASTA)has strong antioxidant properties and has been widely used as a health product to improve human health.However,the effects of ASTA on the reproductive performance of aging roosters ...Background:Natural astaxanthin(ASTA)has strong antioxidant properties and has been widely used as a health product to improve human health.However,the effects of ASTA on the reproductive performance of aging roosters have been poorly studied.We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via antioxidation defense system.Methods:In the present study,9653-week-old Jinghong No.1 layer breeder roosters were fed a corn-soybean meal basal diet containing 0,25,50,or 100 mg/kg ASTA for 6 weeks.Results:Semen quality in the ASTA groups remarkably improved than that in the control group,and antioxidant activities,the abilities to scavenge hydroxyl radicals and superoxide anions,increased gradually with ASTA addition(P<0.05).In addition,the mRNA levels of antioxidant enzymes as well as the mRNA and protein levels of the mitogen-activated protein kinase(MAPK)and nuclear factor-erythroid 2-related factor 2(Nrf2)were markedly increased in the 50-100 mg/kg ASTA group(P<0.05).Conclusions:Collectively,these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities and the ability to scavenge hydroxyl radicals,which may be related to upregulation of the MAPK/Nrf2 pathway.展开更多
This paper proposes a multi-axis projection (MAP) based giant component formation strategy via the Maximal Independent Set (MIS) in a random unit-disk graph.We focus on the problem of virtual back-bone construction in...This paper proposes a multi-axis projection (MAP) based giant component formation strategy via the Maximal Independent Set (MIS) in a random unit-disk graph.We focus on the problem of virtual back-bone construction in wireless ad hoc and sensor networks,where the coverage areas of the nodes are disks with identical radii.In the simulation,we show that the MAP-based giant component has the ability to connect most nodes and serves as a backbone in the network.The algorithm is localized and may play an important role in efficiently constructing a virtual backbone for ad hoc and sensor networks.展开更多
Objective.Several studies examined the putativefets of SARS-Cov-2infectionon spem parametersHoweve,the resuls remain controversial.In this study,we conducted the most up-to-date systematic review and meta-analysis to ...Objective.Several studies examined the putativefets of SARS-Cov-2infectionon spem parametersHoweve,the resuls remain controversial.In this study,we conducted the most up-to-date systematic review and meta-analysis to investigate the effect of SARS-CoV-2 infection on sperm quality in COVID-19-positive and COVID-19-negative male participants.Method.Seven databases were searched forliterature released through June 10,2022,containing estimates for the outcomes of interest Using a random-fects model(REM)or a fixed-ffects model(FEM),we analyzed the pooled results.The quality of all included studies was asssed by the Newcastle-Ottawa scale In addition,we performed aquanttative and subgroup analysis of semen data across all included studies.Results.Fourteen studies were extracted from 10 publications,involving a total of 1174 participates for meta-analysis.Sperm parameters of 521 COVID-19 male patients and 653 controls were analyzed.In 8 case-control studies,the pooled mean dfference(MD)of total sperm motility was-5.37%(95%confidence interval(CI):-8.47 to-2.28:<0.05),suggesting that total motility was significantly impaired inmale COVID-19 cases.Subgroup analysis showed a significant decreasein semen volume,sperm concentration,and total motility in 238 patients with arecovery timeof less than 90 days.Moreover,in the other 6 included pre-to post-COVID-19 studies,the pooled MDs of sperm concentration,total sperm count,total motility,progressivemotility,and normal morphology were6.54×10^(6)/ml(95%CI:-10.27 to-2.81;p<0.05),38.89×10^(6)(95%CI:-59.20 to-18.58;p<0.05),-7.21%(95%CI:-14.36 to-0.07;p<0.05),-5.12%(95%CI:-8.71 to-1.53;p<0.05),and-1.52%(95%CI:-2.88 to-0.16;p<0.05),respectively,which indicate SARS-CoV-2 infection significantly affected these five sperm parameters.Conclusion.Our results revealed that SARS-CoV-2 infection was significantly correlated with decreased sperm quality.Of six sperm parameters,total motility and sperm concentration were the most significantly decreased parameters.These results suggest a possible negative influence of SARS-CoV-2 infection on testicular function and male fertility.Given the potential detrimental effect of SARS-CoV-2 on semen quality,male reproductive health should be monitored closely in patients with COVID-19.This trial is registered with CRD42021275823.展开更多
基金financially supported by the innovation research team for modern agricultural industry and technology in Beijing
文摘Background:Fatness traits in animals are important for their growth,meat quality,reproductive performance,and immunity.The liver is the principal organ of the regulation of lipid metabolism,and this study used massive parallelized high-throughput sequencing technologies to determine the porcine liver tissue transcriptome architecture of two full-sibling Songliao black pigs harboring extremely different phenotypes of backfat thickness.Results:The total number of reads produced for each sample was in the region of 53 million,and 8,226 novel transcripts were detected.Approximately 92 genes were differentially regulated in the liver tissue,while 31 spliced transcripts and 33 primary transcripts showed significantly differential expression between pigs with higher and lower backfat thickness.Genes that were differentially expressed were involved in the metabolism of various substances,small molecule biochemistry,and molecular transport.Conclusions:Genes involved in the regulation of lipids could play an important role in lipid and fatty acid metabolism in the liver.These results could help us understand how liver metabolism affects the backfat thickness of pigs.
基金financially supported by the National Key Research and Development Project(2019YFE0106800)the National Key R&D Program of China(2018YFD0501000)+2 种基金Beijing Municipal Education Commission Science and Technology Program General Project(KM201910020010)Beijing Innovation Consortium of Agriculture Research System(BAIC02-2021)the China Agriculture Research System(CARS-35).
文摘Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting consumers’choice of pork.Weighted gene co-expression network analysis(WGCNA)is effective in pig genetic studies.Therefore,this study aimed to identify modules that co-express genes associated with fat deposition in pigs(Songliao black and Landrace breeds)with extreme levels of backfat(high and low)and to identify the core genes in each of these modules.Results:We used RNA sequences generated in different pig tissues to construct a gene expression matrix consisting of 12,862 genes from 36 samples.Eleven co-expression modules were identified using WGCNA and the number of genes in these modules ranged from 39 to 3,363.Four co-expression modules were significantly correlated with backfat thickness.A total of 16 genes(RAD9A,IGF2R,SCAP,TCAP,SMYD1,PFKM,DGAT1,GPS2,IGF1,MAPK8,FABP,FABP5,LEPR,UCP3,APOF,and FASN)were associated with fat deposition.Conclusions:RAD9A,TCAP,SMYD1,PFKM,GPS2,and APOF were the key genes in the four modules based on the degree of gene connectivity.Combining these results with those from differential gene analysis,SMYD1 and PFKM were proposed as strong candidate genes for body size traits.This study explored the key genes that regulate porcine fat deposition and lays the foundation for further research into the molecular regulatory mechanisms underlying porcine fat deposition.
基金This study was supported by Modern Agricultural Industry Technology System-Peking Poultry Innovation Team(BAIC04–2021)the National Key R&D Program of China(2016YFD0700201).
文摘Background:Natural astaxanthin(ASTA)has strong antioxidant properties and has been widely used as a health product to improve human health.However,the effects of ASTA on the reproductive performance of aging roosters have been poorly studied.We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via antioxidation defense system.Methods:In the present study,9653-week-old Jinghong No.1 layer breeder roosters were fed a corn-soybean meal basal diet containing 0,25,50,or 100 mg/kg ASTA for 6 weeks.Results:Semen quality in the ASTA groups remarkably improved than that in the control group,and antioxidant activities,the abilities to scavenge hydroxyl radicals and superoxide anions,increased gradually with ASTA addition(P<0.05).In addition,the mRNA levels of antioxidant enzymes as well as the mRNA and protein levels of the mitogen-activated protein kinase(MAPK)and nuclear factor-erythroid 2-related factor 2(Nrf2)were markedly increased in the 50-100 mg/kg ASTA group(P<0.05).Conclusions:Collectively,these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities and the ability to scavenge hydroxyl radicals,which may be related to upregulation of the MAPK/Nrf2 pathway.
基金Supported by the National Natural Science Foundation of China(No. 60903055)the China Postdoctoral Science Foundation Funded Project (No. 20080430776)the National Basic Research and Development (973) Program of China (No. 2011CB302905)
文摘This paper proposes a multi-axis projection (MAP) based giant component formation strategy via the Maximal Independent Set (MIS) in a random unit-disk graph.We focus on the problem of virtual back-bone construction in wireless ad hoc and sensor networks,where the coverage areas of the nodes are disks with identical radii.In the simulation,we show that the MAP-based giant component has the ability to connect most nodes and serves as a backbone in the network.The algorithm is localized and may play an important role in efficiently constructing a virtual backbone for ad hoc and sensor networks.
基金supported by the National Natural Science Foundation of China(Nos.81801540,81771662and 82101749)the Fundamental Research Funds for the Central Universities(No.2042021kf0082).
文摘Objective.Several studies examined the putativefets of SARS-Cov-2infectionon spem parametersHoweve,the resuls remain controversial.In this study,we conducted the most up-to-date systematic review and meta-analysis to investigate the effect of SARS-CoV-2 infection on sperm quality in COVID-19-positive and COVID-19-negative male participants.Method.Seven databases were searched forliterature released through June 10,2022,containing estimates for the outcomes of interest Using a random-fects model(REM)or a fixed-ffects model(FEM),we analyzed the pooled results.The quality of all included studies was asssed by the Newcastle-Ottawa scale In addition,we performed aquanttative and subgroup analysis of semen data across all included studies.Results.Fourteen studies were extracted from 10 publications,involving a total of 1174 participates for meta-analysis.Sperm parameters of 521 COVID-19 male patients and 653 controls were analyzed.In 8 case-control studies,the pooled mean dfference(MD)of total sperm motility was-5.37%(95%confidence interval(CI):-8.47 to-2.28:<0.05),suggesting that total motility was significantly impaired inmale COVID-19 cases.Subgroup analysis showed a significant decreasein semen volume,sperm concentration,and total motility in 238 patients with arecovery timeof less than 90 days.Moreover,in the other 6 included pre-to post-COVID-19 studies,the pooled MDs of sperm concentration,total sperm count,total motility,progressivemotility,and normal morphology were6.54×10^(6)/ml(95%CI:-10.27 to-2.81;p<0.05),38.89×10^(6)(95%CI:-59.20 to-18.58;p<0.05),-7.21%(95%CI:-14.36 to-0.07;p<0.05),-5.12%(95%CI:-8.71 to-1.53;p<0.05),and-1.52%(95%CI:-2.88 to-0.16;p<0.05),respectively,which indicate SARS-CoV-2 infection significantly affected these five sperm parameters.Conclusion.Our results revealed that SARS-CoV-2 infection was significantly correlated with decreased sperm quality.Of six sperm parameters,total motility and sperm concentration were the most significantly decreased parameters.These results suggest a possible negative influence of SARS-CoV-2 infection on testicular function and male fertility.Given the potential detrimental effect of SARS-CoV-2 on semen quality,male reproductive health should be monitored closely in patients with COVID-19.This trial is registered with CRD42021275823.
