Potential targets and mechanism of Xingxiao Pill for the treatment of lung cancer were analyzed based on network pharmacology and molecular docking

Objective:To analyze the potential targets and mechanism of Xingxiao Pill for the treatment of lung cancer based on network pharmacology and molecular docking,providing reference for the research and clinical development of Xingxiao Pill.Method:The main chemical constituents and their targets of Xingxiao Pill was obtained through BATMAN-TCM database.Effective compounds were screened based on SwissADME and their targets were predicted by SwissTargetPrediction.Main lung cancer targets were obtained and integrated through GeneCards,OMIM(OMIM-Gene-Map-Retrieval),TTD and DRUGBANK databases.The intersection targets of Xingxiao Pill for lung cancer were obtained by Venn.The String platform was used to obtain the intersection targets.The core targets of Xingxiao Pill for lung cancer were screened by BisoGenet3.0.The Metasacpe database was used to analyze the biological processes and pathways involved in intersection targets.Cytoscape3.7.2 software was used to construct the"active ingredients of Xingxiao Pill-targets-pathways"network.Finally,Autodock was used for molecular docking verification.Results:The core active components of Xingxiao Pill for the treatment of lung cancer were morin,testosterone,17-Beta-Estradiol,alphaEstradiol,muscone,commiferin,commisterone,octyl acetate,etc.The primary pathways mainly included PI3K/Akt,MTOR,MAPK signaling pathway and MicroRNAs in cancer,etc.It has been proved that the combination of testosterone and estradiol with AKT1,SIRT7 and MDM2 was stable and could be used as reference.Conclusion:This study preliminarily revealed the mechanism of Xingxiao Pill on treating lung cancer with multiple components,targets and pathways,providing some ideas and references for clinical application and basic research in the future.

Experimental study on the effect of cryoablation on lung cancer mice based on MAPK/ERK signaling pathway

Objective:To study the regulatory effect of cryoablation on MAPK/ERK signaling pathway in mice with lung adenocarcinoma.Methods:Lewis mouse lung adenocarcinoma cell line was used to establish subcutaneous transplanted tumor model in C57BL/6 mice.Ten mice were randomly divided into two groups:sham operation group and cryoablation group,with 5 mice in each group.The cryoablation group was treated with double circulation-rewarming ablation,and the sham operation group was treated with incision and suture at the transplanted tumor.The tumor tissues were taken 14 days after operation.Detect the effect of cryoablation on MAPK/ERK pathway related proteins by Western blot,such as KRAS,RAF1,MEK1,ERK1/2,P-RAF1,P-MEK1,P-ERK1/2.The expression of KRAS gene was further verified by qRt-PCR.Results:Compared with the sham operation group,the phosphorylated proteins P-RAF1,P-MEK1 and P-ERK1/2 in tumor tissue after cryoablation were decreased(P<0.05),and the key molecule KRAS in MAPK/ERK pathway was decreased in protein and gene expression(P<0.05).Conclusion:Cryoablation can negatively regulate MAPK/ERK signaling pathway by down-regulating KRas expression.

The value of preoperative PLR and NLR in the prognostic evaluation of lung cancer

Objective:To explore the relationship between platelet/lymphocyte ratio(PLR)and neutrophil/lymphocyte ratio(NLR)and prognosis of lung cancer patients before cold ablation.Methods:Retrospectively analyze the case data of primary lung cancer patients who underwent cryoablation of lung argon helium knife in our hospital from January 2012 to March 2018.PLR and NLR were calculated based on the blood routine data of the patients within 1 week before the operation.value.The patients were followed up.The deadline for follow-up was June 2021.The survival curve(ROC curve)was drawn according to the patient's survival prognosis,and the Youden index was calculated to determine the optimal cutoff value of PLR and NLR,and grouped based on this analysis.The correlation between pre-PLR level and NLR level with clinicopathological characteristics and prognostic survival time of patients with primary lung cancer.Results:The best cut-off point was calculated according to the ROC curve and divided into high PLR group(PLR≥155.72,n=127)and low PLR group(<155.72,n=87);high NLR group(≥2.91,n=120)and Low NLR group(<2.91,n=94);preoperative high PLR,high NLR group pT,pN,pM and TNM staging compared with low value group,the difference was statistically significant(P<0.05);Cox multivariate Analysis shows that high PLR,high NLR,high pT staging,high pM staging,and high TNM staging are independent risk factors affecting postoperative survival;the postoperative survival rate of the high-level PLRNLR group was significantly lower than that of the other groups,and the difference was statistically significant.significance.Conclusion:The significant increase of NLR and PLT before operation in patients with primary lung cancer is associated with poor prognosis;the combination of the two has potential clinical application value in assessing the survival of patients after surgery.