Dedifferentiation of Schwann cells is an important feature of the response to peripheral nerve injury and specific negative myelination reg- ulators are considered to have a major role in this process. However, most e...Dedifferentiation of Schwann cells is an important feature of the response to peripheral nerve injury and specific negative myelination reg- ulators are considered to have a major role in this process. However, most experiments have focused on the distal nerve stump, where the Notch signaling pathway is strongly associated with Schwann cell dedifferentiation and repair of the nerve. We observed the phenotypic changes of Schwann cells and changes of active Notch signaling on the proximal stump during peripheral nerve repair using small gap conduit tubulization. Eighty rats, with right sciatic nerve section of 4 mm, were randomly assigned to conduit bridging group and control group （epineurium suture）. Glial fibrillary acidic protein expression, in myelinating Schwann cells on the proximal stump, began to up-reg- ulate at 1 day after injury and was still evident at 5 days. Compared with the control group, Notchl mRNA was expressed at a higher level in the conduit bridging group during the first week on the proximal stump. Hesl mRNA levels in the conduit bridging group significantly increased compared with the control group at 3, 5, 7 and 14 days post-surgery. The change of the Notch intracellular domain shared a simi- lar trend as Hesl mRNA expression. Our results confirmed that phenotypic changes of Schwann cells occurred in the proximal stump. The differences in these changes between the conduit tubulization and epineurium suture groups correlate with changes in Notch signaling. This suggests that active Notch signaling might be a key mechanism during the early stage of neural regeneration in the proximal nerve stump.展开更多
Aim:To study the expression of PANDA,LincRNA-p21,and PUMA in lung tissue of patients with lung cancer from Xuanwei of Yunnan Province.Methods:Forty-five cases of lung cancer patients from Xuanwei and 42 lung cancer ca...Aim:To study the expression of PANDA,LincRNA-p21,and PUMA in lung tissue of patients with lung cancer from Xuanwei of Yunnan Province.Methods:Forty-five cases of lung cancer patients from Xuanwei and 42 lung cancer cases from non-Xuanwei were enrolled.Extraction of RNA was done using the Trizol kit.Real-time fluorescence quantitative PCR assay was done to obtain the relative expression.Results:Expressions of PANDA,LincRNA-p21,and PUMA in male and female patients or in squamous cell carcinoma and adenocarcinoma were not significantly different(P>0.05).However,expression of LincRNA-p21 in Xuanwei patients was higher than non-Xuanwei patients(P<0.05).Expression of PUMA in tumor tissue was lower than that in normal lung tissue(P<0.05),and i n Xuanwei patients was lower than non-Xuanwei patients(P<0.05).In patients from non-Xuanwei regions,expression of LincRNA-p21 in patients with smoking index>400 was higher than in those<400 and non-smokers.Conclusion:Expressions of PANDA,LincRNA-p21,and PUMA in lung tissues have no gender differences or tissue specificity.High expression of LincRNA-p21 in Xuanwei patients may have relationship with cell damage caused by coal burning pollution in Xuanwei.展开更多
基金supported by the Shandong Science and Research Foundation for Youth Scientists in China,No.BS2012YY019
文摘Dedifferentiation of Schwann cells is an important feature of the response to peripheral nerve injury and specific negative myelination reg- ulators are considered to have a major role in this process. However, most experiments have focused on the distal nerve stump, where the Notch signaling pathway is strongly associated with Schwann cell dedifferentiation and repair of the nerve. We observed the phenotypic changes of Schwann cells and changes of active Notch signaling on the proximal stump during peripheral nerve repair using small gap conduit tubulization. Eighty rats, with right sciatic nerve section of 4 mm, were randomly assigned to conduit bridging group and control group （epineurium suture）. Glial fibrillary acidic protein expression, in myelinating Schwann cells on the proximal stump, began to up-reg- ulate at 1 day after injury and was still evident at 5 days. Compared with the control group, Notchl mRNA was expressed at a higher level in the conduit bridging group during the first week on the proximal stump. Hesl mRNA levels in the conduit bridging group significantly increased compared with the control group at 3, 5, 7 and 14 days post-surgery. The change of the Notch intracellular domain shared a simi- lar trend as Hesl mRNA expression. Our results confirmed that phenotypic changes of Schwann cells occurred in the proximal stump. The differences in these changes between the conduit tubulization and epineurium suture groups correlate with changes in Notch signaling. This suggests that active Notch signaling might be a key mechanism during the early stage of neural regeneration in the proximal nerve stump.
基金Yunnan Provincial Department of Science and Technology.Project:Use EBUS-TBNA to detect the expression of MALAT1,MYC,PVT1 genes in lymph node of NSCLC patient(No.2016NS108)Project:Study on the mechanism of regulation of miRNAs and its target genes in Gejiu and Xuanwei NSCLC patients(No.2014NS002).
文摘Aim:To study the expression of PANDA,LincRNA-p21,and PUMA in lung tissue of patients with lung cancer from Xuanwei of Yunnan Province.Methods:Forty-five cases of lung cancer patients from Xuanwei and 42 lung cancer cases from non-Xuanwei were enrolled.Extraction of RNA was done using the Trizol kit.Real-time fluorescence quantitative PCR assay was done to obtain the relative expression.Results:Expressions of PANDA,LincRNA-p21,and PUMA in male and female patients or in squamous cell carcinoma and adenocarcinoma were not significantly different(P>0.05).However,expression of LincRNA-p21 in Xuanwei patients was higher than non-Xuanwei patients(P<0.05).Expression of PUMA in tumor tissue was lower than that in normal lung tissue(P<0.05),and i n Xuanwei patients was lower than non-Xuanwei patients(P<0.05).In patients from non-Xuanwei regions,expression of LincRNA-p21 in patients with smoking index>400 was higher than in those<400 and non-smokers.Conclusion:Expressions of PANDA,LincRNA-p21,and PUMA in lung tissues have no gender differences or tissue specificity.High expression of LincRNA-p21 in Xuanwei patients may have relationship with cell damage caused by coal burning pollution in Xuanwei.
