Hepatocellular carcinoma(HCC) is a prevalent and highly malignant cancer throughout the world.Effective treatment of this disease is impeded by the high rate of metastasis, recurrence, and chemoresistance. Recent stud...Hepatocellular carcinoma(HCC) is a prevalent and highly malignant cancer throughout the world.Effective treatment of this disease is impeded by the high rate of metastasis, recurrence, and chemoresistance. Recent studies have revealed the close relationship between the malignant phenotype of HCC and cancer stem cells(CSCs). Therefore, CSC-targeted therapy is considered a promising strategy to eradicate HCC. Traditional Chinese medicine(TCM) can be effective in preventing recurrence and metastasis of some advanced HCC. A growing amount of literature has discovered that extracts or compounds derived from TCM exert an anti-CSC effect. This review introduces some formulas and chemical compounds derived from TCMs that have been reported to inhibit CSCs of HCC;these TCM-related drugs may help to provide an alternative approach to help manage cancers, especially for HCC which has a great potential of metastasis, recurrence, and chemoresistance.展开更多
Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and oviposition.The odorant receptor co-receptor(Orco)is an obligatory odorant receptor and indispensable in odor perception.H...Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and oviposition.The odorant receptor co-receptor(Orco)is an obligatory odorant receptor and indispensable in odor perception.Here,we characterized the Orco gene from the oriental fruit fly,Bactrocera dorsalis(Hendel),a notorious agriculture pest.The olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 system.Electroantennograms(EAG)and olfactory preference assays confirmed that BdorOrco^(−/−)mutant flies had reduced perception of methyl eugenol,β-caryophyllene,and ethyl acetate.Oviposition bioassays showed that the eggs laid by BdorOrco^(−/−)females mediated by benzothiazole and 1-octen-3-ol were significantly decreased.In addition,BdorOrco^(−/−)mutant flies took a significantly longer time to locate the food source compared with wild type(WT)flies.Altogether,our data indicated that Orco is essential for multiple physiological processes in B.dorsalis,and it expands our understanding of the function of insect Orco.展开更多
基金supported by the National Natural Science Foundation of China (No. 81573894),which paid for planning and collection of literaturethe Budget Research Program of Shanghai Municipal Education Commission (No. 18LK027),which paid for writing and submitting the article for publication.
文摘Hepatocellular carcinoma(HCC) is a prevalent and highly malignant cancer throughout the world.Effective treatment of this disease is impeded by the high rate of metastasis, recurrence, and chemoresistance. Recent studies have revealed the close relationship between the malignant phenotype of HCC and cancer stem cells(CSCs). Therefore, CSC-targeted therapy is considered a promising strategy to eradicate HCC. Traditional Chinese medicine(TCM) can be effective in preventing recurrence and metastasis of some advanced HCC. A growing amount of literature has discovered that extracts or compounds derived from TCM exert an anti-CSC effect. This review introduces some formulas and chemical compounds derived from TCMs that have been reported to inhibit CSCs of HCC;these TCM-related drugs may help to provide an alternative approach to help manage cancers, especially for HCC which has a great potential of metastasis, recurrence, and chemoresistance.
基金supported by funding from the National Natural Science Foundation of China(31772233,32072491)Fok Ying Tung Education Foundation(161029)+1 种基金Chongqing Natural Science Foundation(cstc2019jcyj-zdxmX0034)Chongqing Young Talents Support Program and the China Agriculture Research System of MOF and MARA.
文摘Olfaction plays an essential role in insect behavior such as host location,foraging,mating,and oviposition.The odorant receptor co-receptor(Orco)is an obligatory odorant receptor and indispensable in odor perception.Here,we characterized the Orco gene from the oriental fruit fly,Bactrocera dorsalis(Hendel),a notorious agriculture pest.The olfactory deficiency mutants were generated by editing the BdorOrco gene using the CRISPR/Cas9 system.Electroantennograms(EAG)and olfactory preference assays confirmed that BdorOrco^(−/−)mutant flies had reduced perception of methyl eugenol,β-caryophyllene,and ethyl acetate.Oviposition bioassays showed that the eggs laid by BdorOrco^(−/−)females mediated by benzothiazole and 1-octen-3-ol were significantly decreased.In addition,BdorOrco^(−/−)mutant flies took a significantly longer time to locate the food source compared with wild type(WT)flies.Altogether,our data indicated that Orco is essential for multiple physiological processes in B.dorsalis,and it expands our understanding of the function of insect Orco.
