To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the ...To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PGR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation.展开更多
In order to reduce or avoid the fluctuations from interface breakup, a meandering microchannel with curved multi-bends(44 turns) is fabricated, and investigations of scaling bubble/slug length in Taylor flow in a rect...In order to reduce or avoid the fluctuations from interface breakup, a meandering microchannel with curved multi-bends(44 turns) is fabricated, and investigations of scaling bubble/slug length in Taylor flow in a rectangular meandering microchannel are systematically conducted. Based on considerable experimental data,quantitative analyses for the influences of two important characteristic times, liquid phase physical properties and aspect ratio are made on the prediction criteria for the bubble/slug length of Taylor flow in a meandering microchannel. A simple principle is suggested to predict the bubble formation period by using the information of Rayleigh time and capillary time for six gas–liquid systems with average deviation of 10.96%. Considering physical properties of the liquid phase and cross-section configuration of the rectangular mcirochannel,revised scaling laws for bubble length are established by introducing Ca, We, Re and W/h whether for the squeezing-driven or shearing-driven of bubble break. In addition, a simple principle in terms of Garstecki-type model and bubble formation period is set-up to predict slug lengths. A total of 107 sets of experimental data are correlated with the meandering microchannel and operating range: 0.001 b CaTPb 0.05, 0.06 b WeTPb 9.0,18 b ReTPb 460 using the bubble/slug length prediction equation from current work. The average deviation between the correlated data and the experimental data for bubble length and slug length is about 9.42% and9.95%, respectively.展开更多
A synthetic method of porphyrins tailed with salicylic substituents is described. Reaction of bromoalkoxyphenyl porphyrin 1 with salicylic acid gave porphyrins 2~5. These new compounds were confirmed by 1H NMR, IR, ...A synthetic method of porphyrins tailed with salicylic substituents is described. Reaction of bromoalkoxyphenyl porphyrin 1 with salicylic acid gave porphyrins 2~5. These new compounds were confirmed by 1H NMR, IR, UV-vis, MS and elemental analysis, and observed their interaction with bovine serum albumin (BSA) in fluorescence spectrum.展开更多
The large amount of repeats, especially high copy repeats, in the genomes of higher animals and plants makes whole genome assembly (WGA) quite difficult. In order to solve this problem, we tried to identify repeats an...The large amount of repeats, especially high copy repeats, in the genomes of higher animals and plants makes whole genome assembly (WGA) quite difficult. In order to solve this problem, we tried to identify repeats and mask them prior to assembly even at the stage of genome survey. It is known that repeats of different copy number have different probabilities of appearance in shotgun data, so based on this principle, we constructed a statistical model and inferred criteria for mathematically defined repeats (MDRs) at different shotgun coverages. According to these criteria, we developed software MDRmasker to identify and mask MDRs in shotgun data. With repeats masked prior to assembly, the speed of assembly was increased with lower error probability. In addition, clone-insert size affects the accuracy of repeat assembly and scaffold construction. We also designed length distribution of clone-inserts using our model. In our simulated genomes of human and rice, the length distribution of repeats is different, so their optimal length distributions of clone-inserts were not the same. Thus with optimal length distribution of clone-inserts, a given genome could be assembled better at lower coverage.展开更多
This paper looks at the heavy-haul combined train composed of different types of locomotives and its distributed power control system with a combination of 800 MHz and TD-LTE wireless communication.It analyses some ke...This paper looks at the heavy-haul combined train composed of different types of locomotives and its distributed power control system with a combination of 800 MHz and TD-LTE wireless communication.It analyses some key characteristic parameters that affect the synchronism and communication of the differential wireless multi-traction synchronous control systems for heavy-haul combined trains.At the same time,in order to reduce the latency of instruction and information transfer between different types of locomotives,improve the time-limit certainty of wireless transmission and optimize the control quality of multi-traction control systems for heavy-haul combined trains,a synchronism optimization strategy based on the Markov decision process on the basis of Petri networkconstruction is proposed.Relevant experiments and tests are carried out to verify the effectiveness of the synchronism optimization of the control system,which provides a guarantee for improving the differential wireless multi-traction synchronous control system for combined trains and optimizing train control.展开更多
基金supported by grants from the Major State Basic Research Development Program of China(No.001CB5099)the National High Technology Research and Development Program of China(No.2001AA216121)+3 种基金National Natural Science Foundation of China(No.30040003)Projects of Shanghai Science&Technology Development Foundation(No.99DJ14002,00DJ14033,01DJ14003)the Chinese Academy of Sciences(No.KSCX-2-3-08)Shanghai Municipal Education Commission and by Shanghai Second Medical University
文摘To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PGR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation.
基金Supported by the National Natural Science Foundation of China(21476037,21606034).
文摘In order to reduce or avoid the fluctuations from interface breakup, a meandering microchannel with curved multi-bends(44 turns) is fabricated, and investigations of scaling bubble/slug length in Taylor flow in a rectangular meandering microchannel are systematically conducted. Based on considerable experimental data,quantitative analyses for the influences of two important characteristic times, liquid phase physical properties and aspect ratio are made on the prediction criteria for the bubble/slug length of Taylor flow in a meandering microchannel. A simple principle is suggested to predict the bubble formation period by using the information of Rayleigh time and capillary time for six gas–liquid systems with average deviation of 10.96%. Considering physical properties of the liquid phase and cross-section configuration of the rectangular mcirochannel,revised scaling laws for bubble length are established by introducing Ca, We, Re and W/h whether for the squeezing-driven or shearing-driven of bubble break. In addition, a simple principle in terms of Garstecki-type model and bubble formation period is set-up to predict slug lengths. A total of 107 sets of experimental data are correlated with the meandering microchannel and operating range: 0.001 b CaTPb 0.05, 0.06 b WeTPb 9.0,18 b ReTPb 460 using the bubble/slug length prediction equation from current work. The average deviation between the correlated data and the experimental data for bubble length and slug length is about 9.42% and9.95%, respectively.
文摘A synthetic method of porphyrins tailed with salicylic substituents is described. Reaction of bromoalkoxyphenyl porphyrin 1 with salicylic acid gave porphyrins 2~5. These new compounds were confirmed by 1H NMR, IR, UV-vis, MS and elemental analysis, and observed their interaction with bovine serum albumin (BSA) in fluorescence spectrum.
文摘The large amount of repeats, especially high copy repeats, in the genomes of higher animals and plants makes whole genome assembly (WGA) quite difficult. In order to solve this problem, we tried to identify repeats and mask them prior to assembly even at the stage of genome survey. It is known that repeats of different copy number have different probabilities of appearance in shotgun data, so based on this principle, we constructed a statistical model and inferred criteria for mathematically defined repeats (MDRs) at different shotgun coverages. According to these criteria, we developed software MDRmasker to identify and mask MDRs in shotgun data. With repeats masked prior to assembly, the speed of assembly was increased with lower error probability. In addition, clone-insert size affects the accuracy of repeat assembly and scaffold construction. We also designed length distribution of clone-inserts using our model. In our simulated genomes of human and rice, the length distribution of repeats is different, so their optimal length distributions of clone-inserts were not the same. Thus with optimal length distribution of clone-inserts, a given genome could be assembled better at lower coverage.
基金supported by National Key R&D Programof China(Grand No.2017YFB1201302-13).
文摘This paper looks at the heavy-haul combined train composed of different types of locomotives and its distributed power control system with a combination of 800 MHz and TD-LTE wireless communication.It analyses some key characteristic parameters that affect the synchronism and communication of the differential wireless multi-traction synchronous control systems for heavy-haul combined trains.At the same time,in order to reduce the latency of instruction and information transfer between different types of locomotives,improve the time-limit certainty of wireless transmission and optimize the control quality of multi-traction control systems for heavy-haul combined trains,a synchronism optimization strategy based on the Markov decision process on the basis of Petri networkconstruction is proposed.Relevant experiments and tests are carried out to verify the effectiveness of the synchronism optimization of the control system,which provides a guarantee for improving the differential wireless multi-traction synchronous control system for combined trains and optimizing train control.