Separation of Peptides by Pressurized Capillary Electrochromatography

A novel gradient pressurized capillary electrochromatography (pCEC) instrument was developed to separate peptides. Two gradient elution modes, hydrophobic and hydrophilic interaction mode in pCEC, were performed on this instrument. Baseline separation of six peptides was obtained on two gradient modes with C18 column and strong cationic exchange column respectively. The effects of mixer volume and total flow rate of pumps on resolution were also discussed.

Preparation of Monolithic Column and Its Application in p-CEC

A novel monolithic stationary phase having long alkyl chain ligands was introduced and evaluated in pressurized-capillary electrochromatography of small neutral and charged compounds. The monolithic column was prepared by the in situ copolymerization of ethylene dimethacrylate, 1-hexadecene, allyl alcohol and 2-acrylamido-2-methyl-1-propanesulfonic acid in a quaternary porogenic solvent mixture consisting of 1, 4-butanediol, cyclohexanol, dodecanol and water.

Exploration of Diversified Experimental Teaching mode in Computer Specialty

As the key link of cultivating the talents, the experimental teaclamg laas become an ~mportant part m me system o~ me innovation in higher education. And it plays an indispensable role in the teaching system of the computer specialty. The article analyzes the current situation of the experimental teaching in computer specialty, and puts forward the reform measures and methods of the diversified experimental teaching, which can effectively improve teaching effect.

Stimulation of Anterior Thalamic Nuclei Protects Against Seizures and Neuronal Apoptosis in Hippocampal CA3 Region of Kainic Acid-induced Epileptic Rats

Background：The antiepileptic effect of the anterior thalamic nuclei （ANT） stimulation has been demonstrated;however,its underlying mechanism remains unclear.The aim of this study was to investigate the effect of chronic ANT stimulation on hippocampal neuron loss and apoptosis.Methods：Sixty-four rats were divided into four groups：The control group,the kainic acid （KA） group,the sham-deep brain stimulation （DBS） group,and the DBS group.KA was used to induce epilepsy.Seizure count and latency to the first spontaneous seizures were calculated.Nissl staining was used to analyze hippocampal neuronal loss.Polymerase chain reaction and Western blotting were conducted to assess the expression of caspase-3 （Casp3）,B-cell lymphoma-2 （Bcl2）,and Bcl2-associated X protein （Box） in the hippocampal CA3 region.One-way analysis of variance was used to determine the differences between the four groups.Results：The latency to the first spontaneous seizures in the DBS group was significantly longer than that in the KA group （27.50 ± 8.05 vs.16.38 ± 7.25 days,P =0.0005）.The total seizure number in the DBS group was also significantly reduced （DBS vs.KA group：11.75 ± 6.80 vs.23.25 ± 7.72,P =0.0002）.Chronic ANT-DBS reduced neuronal loss in the hippocampal CA3 region （DBS vs.KA group：23.58 ± 6.34 vs.13.13 ± 4.00,P =0.0012）.After chronic DBS,the relative mRNA expression level of Casp3 was decreased （DBS vs.KA group：1.18 ± 0.37 vs.2.09 ± 0.46,P =0.0003）,and the relative mRNA expression level of Bcl2 was increased （DBS vs.KA group：0.92 ± 0.21 vs.0.48 ± 0.16,P =0.0004）.The protein expression levels of CASP3 （DBS vs.KA group：1.25 ± 0.26 vs.2.49 ± 0.38,P 〈 0.0001） and BAX （DBS vs.KA group：1.57 ± 0.49 vs.2.80 ± 0.63,P =0.0012） both declined in the DBS group whereas the protein expression level of BCL2 （DBS vs.KA group：0.78 ± 0.32 vs.0.36 ± 0.17,P =0.0086） increased in the DBS group.Conclusions：This study demonstrated that chronic ANT stimulation could exert a neuroprotective effect on hippocampal neurons.This neuroprotective effect is likely to be mediated by the inhibition of apoptosis in the epileptic hippocampus.

Gas-solid flow in a high-density circulating fluidized bed riser with Geldart group B particles

We carried out experiments to explore and characterize the gas-solid flow dynamics of Geldart group B particles in a dense circulating fluidized bed riser. By reducing the pressure drop across the solid control valve and increasing the solid inventory in the storage tank, a high solid circulation rate and a solid holdup above 0.075 throughout the riser were simultaneously achieved. At a solid-to-gas mass flux ratio of approximately 105, flow transitioned from fast fluidization to a dense suspension upflow. In the axial direction of the riser, solid holdup had an exponential profile, increasing with increasing solid circulation rate and Jot decreasing superficial gas velocity. From the riser＇s center to its wall, the solid holdup increased markedly, exhibiting a steep parabolic profile. Increasing the solid circulation rate increased the radial non-uniformity of the solid concentration, while increasing the superficial gas velocity had the opposite effect, In our dense circulating fluidized bed riser, Geldart group B particles had similar slip characteristics to Geldart group A particles,

An Electrode-preserved Patient after Bilateral Intracranial Hematoma Induced by Deep Brain Stimulation

To the Editor： Deep brain stimulation （DBS） is widely used in Parkinson＇s disease. Here, we report a bilateral hemorrhage case Figure 1： Postoperative computed tomography data indicating the disease evolution in this case. （a） A computed tomography scan 8 h after the first operation; （b） A computed tomography scan 8 h after the second operation; （c） A computed tomography scan 13 day after the second operation.