Determination of 13 Trace Elements in Anshun Thorn Pear (Rosa roxburghii) and Golden Thorn Pear (Rosa sterilis S.D.Shi) by ICP-MS

[Objectives] This study was conducted to establish an analytical method for the establishment of 13 trace elements in Anshun thorn pear(Rosa roxburghii) and golden thorn pear(Rosa sterilis S. D. Shi). [Methods] Nitric acid was used as the digestion system for microwave digestion. Inductively coupled plasma mass spectrometry(ICP-MS) was used to determine the trace elements in thorn pear and golden thorn pear. [Results] Be, Ti, V, Cr, Mn, Co, Ni, As, Sr, Mo, Cd, Sb and Hg were determined by ICP-MS method in thorn pear and golden thorn pear. The correlation coefficients of the regression equations of the elements were higher than 0.996; the precision RSD was lower than 2.87%; and the recovery ranged from 94.3% to 109.8%. [Conclusions] The method has the advantages of high sensitivity, good accuracy and simultaneous detection of multiple elements, and provides a reference method and theoretical basis for determining trace elements in thorn pear and golden thorn pear and other fruits.

Determination of 2 Flavonoids in Psidium guajava Linn. from Different Habitats in Guizhou

[ Objective] This study was conducted to determinate the contents of hyperoside and isoquercitrin in the leaves of Psidium guajava Linn. from different areas of Guizhou Province, which would provide data support for production and quality control of P. guajava leaf material. [ Method] The contents of hypereside and isoquercitrin were measured by HPLC under following conditions： Agilent XDB-C18,4.6 mm x 250 ram, 5 ttm, CH3CN-0.1% HCOOH （13： 87） as mobile phase, column temporature at 25 ℃, detection wavelength of 257 mn and flow rate at 1.0 ml/min. [ Result ] The results showed that leaves of P. guajava from dif- ferent producing areas contained variable hypereaide and isoquercitrin contents in the same period, and the differences in quality were obvious between different are- as. [ Conclusion] The method for detecting the contents of hypereside and isoquercitrin in P. guajava leaf materials is simple with reliable accurate results. The P. guajava leaf material produced in Gouting Township, Luedian County has better quality.

Study on the Quality Standard of Heracleum moellendorffii Hance.

[Objectives]This study was conducted to investigate the quality standard of Heracleum moellendorffii Hance.,so as to provide data support for the 2003 version of Quality Standards for Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province.[Methods]H.moellendorffii was subjected to character identification and microscopic identification.Referring to Chinese Pharmacopoeia,TCL thin-layer identification and extract determination were carried out on 10 batches of H.moellendorffii from 6 habitats in Guizhou.[Results]The characters and microscopic characteristics of H.moellendorffii were described in detail.The TLC thin-layer identification spots were clear,with strong specificity.The limit of the medicinal material extract was determined to be not less than 22.0%.[Conclusions]This study can provide data support for the quality evaluation and standard improvement of the medicinal material H.moellendorffii.

A Preliminary Study on the Quality Standard of Raphanus sativus L. Produced in Guizhou Province

[Objectives]This study was conducted to analyze the Raphanus sativus L.medicinal materials from different origins and batches in Guizhou Province to explore its quality standards.[Methods]The plant morphology,material shape and microscopic characteristics of R.sativus were identified;and qualitative identification was performed by the TLC method.[Results]The plant morphology,material shape and microscopic characteristics of the R.sativus medicinal materials were described;and the TLC identification method of the R.sativus medicinal materials was established,with clear spots,good separation,and good specificity.[Conclusions]The method is accurate,simple,highly sensitive,interference-free,has good reproducibility,and can be used as a data support for the formulation of quality standards for R.sativus medicinal materials.

Characters and Microscopic Identification of Aralia cordata

[Objectives]Characters and microscopic characteristics of Aralia cordata were studied,to improve identification method for Aralia cordata.[Methods]Using pharmacognostic identification method,original plant morphology of Aralia cordata was observed,and microscopic observation of its root cross section and powder was conducted.[Results]It is perennial herb,with rhizome recess,arranged in clusters of nodules.There are double or triple pinnate compound leaves.It is glabrous or sparsely pubescent,with umbel.Flowers are white,while fruits are spherical and purple black.In microstructure,the cork layer on the cross section of root consists of 6-10 rows of cells,and there is secondary cortex.The parenchyma cells are rich in starch granules,and the cambium is not obvious.The vessels are uniserial,and the wood fiber is less,and the primary xylem is bipartite.There are many starch granules in powder,mostly single granules,round or quasi round,oval,umbilicus herringbone or short slit,with many marginal pits and reticulate ducts.Calcium oxalate cluster crystals are mostly gathered.Cork cells are yellowish brown and arranged neatly.The fibrous wall is thin,and the pore groove is obvious,and stone cells are occasionally seen.[Conclusions]Using character and microscopic identification method,it could accurately identify medicinal material of Aralia cordata,and provide reference basis for development and utilization of its resources.

GC-MS Analysis of Chemical Components of Volatile Oil in Different Parts of Fennel( Foenicuzu vulgare Mill. )

[Objectives] This study was conducted to analyze the composition of volatile oil in different parts of fennel(Foenicuzu vulgare Mill.) and to compare the differences in the composition of volatile oil in different parts of fennel.[Methods]The steam distillation method was applied to extract volatile oil from different parts of fennel,and the components of volatile oil from different parts of fennel were separated and identified by GC-MS.The relative content of each component was determined by the peak area normalization method.[Results]37,33,18,and 44 chemical components were separated from the volatile oil of fennel roots,stems,young leaves and fruit,respectively,accounting for 98.64%,99.34%,99.59% and 95.99% of the total volatile oil of corresponding parts.A total of 77 chemical components were identified in the four parts,of which 5 were common components.The main component of the volatile oil in the stems and young leaves was trans-anethole.The main components of the volatile oil in fruit were estragole and trans-anethole.And the main component of the volatile oil in the roots was dill apiol.The components in the volatile oil of fennel roots,stems,young leaves and fruit were different in type and content.[Conclusions]This study provides a theoretical reference for the further effective development and utilization of fennel resources.

A TiO_(2)nanotubes film with excellent antireflective and near-perfect self-cleaning performances

SiO_(2)/TiO_(2)composite films have been frequently used to realize the functions of self-cleaning and antireflection.Increasing the TiO_(2)volume ratio in SiO_(2)/TiO_(2)composite film is beneficial to enhance the self-cleaning effect,while high TiO_(2)content leads to a strong Rayleigh scattering and depresses the antireflective performance,resulting in a bottleneck problem for the dual-functional application.Here,we have achieved a high-quality TiO_(2)nanotubes film with excellent antireflective and near-perfect selfcleaning performances.Ultrasound assisted pickling method has been developed to effectively prepare the well-dispersed protonated titanate nanotubes colloid.After spin-coating and annealing treatment,glass substrate coated with double-side TiO_(2)nanotubes film has a peak transmittance of 99.2%and average transmittance of 97.4%at 400-800 nm.Ultra-high porosity of TiO_(2)nanotubes film(80%)and ultra-fine size of TiO_(2)nanotubes(8.6 nm in outer diameter)lead to excellent antireflective performance.With high UV absorptivity(80%at 254 nm)and formal quantum efficiency of stearic acid(10.9×10^(-3)),TiO_(2)nanotubes film shows near-perfect self-cleaning performance.A persistent anti-fogging ability is also presented.This study demonstrates the feasibility to fabricate pure TiO_(2)antireflective coating for glass substrate,extends application field of the classic TiO_(2)nanotubes,and sheds lights on the practical applications of high-powered TiO_(2)nanotube-based multi-functional films.