Enhancer RNAs(eRNAs)are a class of non-coding RNA,which play a critical role in tumor progression.1 Previous studies have indicated abundant CpG methylation,somatic mutation,and copy number variation in eRNA regions i...Enhancer RNAs(eRNAs)are a class of non-coding RNA,which play a critical role in tumor progression.1 Previous studies have indicated abundant CpG methylation,somatic mutation,and copy number variation in eRNA regions in cancers.2,3 We constructed a landscape of genetic alteration-driven eRNAs and provided an integrative pipeline to identify drug candidates that affect eRNA activity.Furthermore,we explored the prognostic value of genetic alteration-driven eRNAs.展开更多
Triple-negative breast cancer(TNBC)is an aggressive subtype characterized by extensive tumor heterogeneity,with limited treatment options and a poor prognosis.1 We used cell type annotation and trajectory analysis to ...Triple-negative breast cancer(TNBC)is an aggressive subtype characterized by extensive tumor heterogeneity,with limited treatment options and a poor prognosis.1 We used cell type annotation and trajectory analysis to identify two distinct differentiation states of epithelial cells,while tumor cells predominantly resided on one of these branches.Inferring the malignant state of epithelial cells based on copy number variation,280 tumor cells were eventually identified from epithelial cells.Differential genes between tumor cells and non-tumor cells were mainly enriched in immune-related functions and pathways.展开更多
Postsurgical adhesion is a common clinic disease induced by surgical trauma,accompanying serious subsequent complications.Current non-surgical approaches of drugs treatment and biomaterial barrier administration only ...Postsurgical adhesion is a common clinic disease induced by surgical trauma,accompanying serious subsequent complications.Current non-surgical approaches of drugs treatment and biomaterial barrier administration only show limited prevention effects and couldn’t effectively promote peritoneum repair.Herein,inspired by bottlebrush,a novel self-fused,antifouling,and injectable hydrogel is fabricated by the free-radical polymerization in aqueous solution between the methacrylate hyaluronic acid(HA-GMA)and N-(2-hydroxypropyl)methacrylamide(HPMA)monomer without any chemical crosslinkers,termed as H-HPMA hydrogel.The H-HPMA hydrogel can be tuned to perform excellent self-fused properties and suitable abdominal metabolism time.Intriguingly,the introduction of the ultra-hydrophilic HPMA chains to the H-HPMA hydrogel affords an unprecedented antifouling capability.The HPMA chains establish a dense hydrated layer that rapidly prevents the postsurgical adhesions and recurrent adhesions after adhesiolysis in vivo.The H-HPMA hydrogel can repair the peritoneal wound of the rat model within 5 days.Furthermore,an underlying mechanism study reveals that the H-HPMA hydrogel significantly regulated the mesothelial-to-mesenchymal transition(MMT)process dominated by the TGF-β-Smad2/3 signal pathway.Thus,we developed a simple,effective,and available approach to rapidly promote peritoneum regeneration and prevent peritoneal adhesion and adhesion recurrence after adhesiolysis,offering novel design ideas for developing biomaterials to prevent peritoneal adhesion.展开更多
基金the Natural Science Foundation of Heilongjiang Province(China)(LH2023F012)University Excellent Youth Project of Provincial Scientific Research Institute(Heilongjiang,China)(CZKYF2022-1-C006)+1 种基金Outstanding Youth Foundation of Heilongjiang Province(China)(YQ2023F004)Program for Young Scholars with Creative Talents in Heilongjiang Province(China)(UNPYSCT-2020174).
文摘Enhancer RNAs(eRNAs)are a class of non-coding RNA,which play a critical role in tumor progression.1 Previous studies have indicated abundant CpG methylation,somatic mutation,and copy number variation in eRNA regions in cancers.2,3 We constructed a landscape of genetic alteration-driven eRNAs and provided an integrative pipeline to identify drug candidates that affect eRNA activity.Furthermore,we explored the prognostic value of genetic alteration-driven eRNAs.
基金supported by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province,China(No.UNPYSCT-2020174)Excellent Youth Project of Provincial Scientific Research Institute(Heilongjiang,China)(No.CZKYF2022-1-C006)the Hei Long Jiang Postdoctoral Special Foundation(China)(No.LBH-TZ1018).
文摘Triple-negative breast cancer(TNBC)is an aggressive subtype characterized by extensive tumor heterogeneity,with limited treatment options and a poor prognosis.1 We used cell type annotation and trajectory analysis to identify two distinct differentiation states of epithelial cells,while tumor cells predominantly resided on one of these branches.Inferring the malignant state of epithelial cells based on copy number variation,280 tumor cells were eventually identified from epithelial cells.Differential genes between tumor cells and non-tumor cells were mainly enriched in immune-related functions and pathways.
基金supported by the National Natural Science Foundation of China(Grant Nos.81773686,32171336)the Natural Science Foundation of Shaanxi Province(Grant Nos.2021 SF-108,2021 SF-308)。
文摘Postsurgical adhesion is a common clinic disease induced by surgical trauma,accompanying serious subsequent complications.Current non-surgical approaches of drugs treatment and biomaterial barrier administration only show limited prevention effects and couldn’t effectively promote peritoneum repair.Herein,inspired by bottlebrush,a novel self-fused,antifouling,and injectable hydrogel is fabricated by the free-radical polymerization in aqueous solution between the methacrylate hyaluronic acid(HA-GMA)and N-(2-hydroxypropyl)methacrylamide(HPMA)monomer without any chemical crosslinkers,termed as H-HPMA hydrogel.The H-HPMA hydrogel can be tuned to perform excellent self-fused properties and suitable abdominal metabolism time.Intriguingly,the introduction of the ultra-hydrophilic HPMA chains to the H-HPMA hydrogel affords an unprecedented antifouling capability.The HPMA chains establish a dense hydrated layer that rapidly prevents the postsurgical adhesions and recurrent adhesions after adhesiolysis in vivo.The H-HPMA hydrogel can repair the peritoneal wound of the rat model within 5 days.Furthermore,an underlying mechanism study reveals that the H-HPMA hydrogel significantly regulated the mesothelial-to-mesenchymal transition(MMT)process dominated by the TGF-β-Smad2/3 signal pathway.Thus,we developed a simple,effective,and available approach to rapidly promote peritoneum regeneration and prevent peritoneal adhesion and adhesion recurrence after adhesiolysis,offering novel design ideas for developing biomaterials to prevent peritoneal adhesion.
