Extracellular vesicles(EVs)derived from cancer cells are considered as ideal biomarker for liquid biopsy in cancer diagnosis,and are stable and abundant.Electrochemical methods for the detection of EVs are prefe rred ...Extracellular vesicles(EVs)derived from cancer cells are considered as ideal biomarker for liquid biopsy in cancer diagnosis,and are stable and abundant.Electrochemical methods for the detection of EVs are prefe rred over co nventional methods such as Western blotting and enzyme-linked immunosorbent assay for their high sensitivity and real-time detection.This article summaries studies proposing the electrochemical methods utilizing immunological and molecular methodologies for detecting EVs derived biomacromolecules such as miRNAs and transme mbrane protein for cancer diagnosis.Moreover,the electrochemical detection methods are compared and future prospects for the development of electrochemical methods for EVs detection are concluded.展开更多
基金the National Natural Science Foundation of China(Nos.61971216 and 61527806)National Key Research and Development Program of China(No.2017YFA0205301)+3 种基金the Jiangsu Province Medical Talent(No.ZDRCA2016065)the Key Research and Development Project of Jiangsu Province(No.BE2019603)the Health Care for Cadres Research Fund ofjiangsu Province(No.BJ16003)High-level Health Talent Project of Jiangsu Province(No.LGY2019001)。
文摘Extracellular vesicles(EVs)derived from cancer cells are considered as ideal biomarker for liquid biopsy in cancer diagnosis,and are stable and abundant.Electrochemical methods for the detection of EVs are prefe rred over co nventional methods such as Western blotting and enzyme-linked immunosorbent assay for their high sensitivity and real-time detection.This article summaries studies proposing the electrochemical methods utilizing immunological and molecular methodologies for detecting EVs derived biomacromolecules such as miRNAs and transme mbrane protein for cancer diagnosis.Moreover,the electrochemical detection methods are compared and future prospects for the development of electrochemical methods for EVs detection are concluded.
