This paper,for the first time,reports the optimization of the critical medium components for bio-surfactant production from achromobacter xylos strain GSR21 using statistical experimental design.Response surface metho...This paper,for the first time,reports the optimization of the critical medium components for bio-surfactant production from achromobacter xylos strain GSR21 using statistical experimental design.Response surface methodology(RSM)was employed to determine the optimal levels of process variables(agar powder,yeast extract,FeSO_(4)7H_(2)O,and KH_(2)PO_(4)).Central composite design(CCD)of RSM was used to study the four variables at five levels,and bio-surfactant concentration was measured as response.Regression coefficients were calculated by regression analysis,and the model equation was determined.R^(2) value for bio-surfactant(g/L)was tested to be 0.7222,indicating that the model fitted well with the experimental results.Verification of the mathematical model was conducted by performing the experiment with the predicted optimized values,and bio-surfactant yield was found to be 9.69 g/L.Validation of the predicted model was fitted 96.9%with the experimental results conducted under the optimum conditions.Agar powder and yeast extract was identified as efficient components for bio-surfactant(achromobacter xylos GSR21)production.展开更多
文摘This paper,for the first time,reports the optimization of the critical medium components for bio-surfactant production from achromobacter xylos strain GSR21 using statistical experimental design.Response surface methodology(RSM)was employed to determine the optimal levels of process variables(agar powder,yeast extract,FeSO_(4)7H_(2)O,and KH_(2)PO_(4)).Central composite design(CCD)of RSM was used to study the four variables at five levels,and bio-surfactant concentration was measured as response.Regression coefficients were calculated by regression analysis,and the model equation was determined.R^(2) value for bio-surfactant(g/L)was tested to be 0.7222,indicating that the model fitted well with the experimental results.Verification of the mathematical model was conducted by performing the experiment with the predicted optimized values,and bio-surfactant yield was found to be 9.69 g/L.Validation of the predicted model was fitted 96.9%with the experimental results conducted under the optimum conditions.Agar powder and yeast extract was identified as efficient components for bio-surfactant(achromobacter xylos GSR21)production.
