OBJECTIVE To identify potential cell signaling pathways and protein targets of the active compound isolated from Atracylodes lancea "atractylodin" in cholangiocarcinoma,using proteomics approach.METHODS The ...OBJECTIVE To identify potential cell signaling pathways and protein targets of the active compound isolated from Atracylodes lancea "atractylodin" in cholangiocarcinoma,using proteomics approach.METHODS The holangiocar-cinoma cell line was exposed with atractylodin for 3 and 6 h and the proteins from both intra-and extra-cellular components were extracted.The extract proteins were separated by SDS-PAGE and digested with trypsin.The LC-MS/MS was applied to identify proteins.Signaling pathways and protein expression were analyzed by MASCOT and STITCH software.RESULTS A total of 4,323 and 4,318 proteins were identified from intra-and extracellular components,respectively.Six intracellular proteins were linked with the signaling pathways(apoptosis,cell cycle control,and PI3K-AKT).Four extracellular proteins were linked with the signaling pathways(NF-κB and PI3K-AKT).CONCLUSION All these proteins will further study to confirm the link to the anticholangiocarcinoma ac.tivity of actractylodin.展开更多
基金supported by Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma,Chulabhorn International College of Medicine,Thammasat University The National Research University Project of Thailand(NRU)
文摘OBJECTIVE To identify potential cell signaling pathways and protein targets of the active compound isolated from Atracylodes lancea "atractylodin" in cholangiocarcinoma,using proteomics approach.METHODS The holangiocar-cinoma cell line was exposed with atractylodin for 3 and 6 h and the proteins from both intra-and extra-cellular components were extracted.The extract proteins were separated by SDS-PAGE and digested with trypsin.The LC-MS/MS was applied to identify proteins.Signaling pathways and protein expression were analyzed by MASCOT and STITCH software.RESULTS A total of 4,323 and 4,318 proteins were identified from intra-and extracellular components,respectively.Six intracellular proteins were linked with the signaling pathways(apoptosis,cell cycle control,and PI3K-AKT).Four extracellular proteins were linked with the signaling pathways(NF-κB and PI3K-AKT).CONCLUSION All these proteins will further study to confirm the link to the anticholangiocarcinoma ac.tivity of actractylodin.
