核固红、中性红和伊红复染液在含铁血黄素染色中的应用比较

目的本研究旨在比较核固红、中性红和伊红复染液在含铁血黄素染色中的染色效果,为实验室选择更适合常规操作使用的复染剂提供参考。方法对确诊为含铁血黄素阳性的病理切片进行含铁血黄素染色,然后分别使用0.1%核固红、0.5%中性红和1%伊红进行复染。结果使用0.1%核固红进行复染的切片显示出清晰的定位和较强的对比度。结论0.1%核固红复染液在染色效果上表现更佳,有利于病理医生的观察,并对提高含铁血黄素阳性检出率具有实际价值。

黄芪多糖对肉鸡生长性能、免疫功能及屠宰性能的影响

文章旨在研究基础日粮中添加不同水平黄芪多糖对肉鸡生长性能、免疫功能及屠宰性能的影响,试验将200只同养殖场、同批次的1日龄白羽肉鸡随机分为4组,每组5个重复,每个重复10只。其中,对照组肉鸡饲喂玉米-豆粕型常规日粮,其他3组肉鸡分别在常规日粮中添加0.2%(试验1组)、0.4%(试验2组)、0.6%(试验3组)的黄芪多糖,试验为期42 d。结果表明,(1)与对照组相比,试验1、2、3组肉鸡21 d体重显著提高(P<0.05),且试验2组肉鸡1~21 d平均日增重(ADG)、42 d体重、21~42 d平均日增重(ADG)、1~42 d平均日增重(ADG)显著高于其他3组(P<0.05)。(2)与对照组相比,试验1、2、3组肉鸡血清中免疫球蛋白A(IgA)含量显著提高(P<0.05),试验2、3组肉鸡血清中免疫球蛋白M(IgM)含量显著高于对照组(P<0.05);试验1、2、3组肉鸡脾脏指数(SI)显著高于对照组(P<0.05),试验2、3组肉鸡法氏囊指数(BI)显著高于对照组(P<0.05)。(3)与对照组相比,试验1、2、3组肉鸡屠体重(DW)显著提高(P<0.05);与对照组、试验1、3组相比,试验2组肉鸡腿肌重(LMW)显著提高(P<0.05)。结论:黄芪多糖能有效提高肉鸡的生长性能和免疫功能,改善肉鸡屠宰性能,且0.4%黄芪多糖添加水平效果最佳。

Therapeutic effects of menstrual blood-derived endometrial stem cells on mouse models of streptozotocin-induced type 1 diabetes

BACKGROUND Type 1 diabetes(T1D),a chronic metabolic and autoimmune disease,seriously endangers human health.In recent years,mesenchymal stem cell(MSC)transplantation has become an effective treatment for diabetes.Menstrual bloodderived endometrial stem cells(MenSC),a novel MSC type derived from the decidual endometrium during menstruation,are expected to become promising seeding cells for diabetes treatment because of their noninvasive collection procedure,high proliferation rate and high immunomodulation capacity.AIM To comprehensively compare the effects of MenSC and umbilical cord-derived MSC(UcMSC)transplantation on T1D treatment,to further explore the potential mechanism of MSC-based therapies in T1D,and to provide support for the clinical application of MSC in diabetes treatment.METHODS A conventional streptozotocin-induced T1D mouse model was established,and the effects of MenSC and UcMSC transplantation on their blood glucose and serum insulin levels were detected.The morphological and functional changes in the pancreas,liver,kidney,and spleen were analyzed by routine histological and immunohistochemical examinations.Changes in the serum cytokine levels in the model mice were assessed by protein arrays.The expression of target proteins related to pancreatic regeneration and apoptosis was examined by western blot.RESULTS MenSC and UcMSC transplantation significantly improved the blood glucose and serum insulin levels in T1D model mice.Immunofluorescence analysis revealed that the numbers of insulin+and CD31+cells in the pancreas were significantly increased in MSC-treated mice compared with control mice.Subsequent western blot analysis also showed that vascular endothelial growth factor(VEGF),Bcl2,Bcl-xL and Proliferating cell nuclear antigen in pancreatic tissue was significantly upregulated in MSC-treated mice compared with control mice.Additionally,protein arrays indicated that MenSC and UcMSC transplantation significantly downregulated the serum levels of interferonγand tumor necrosis factorαand upregulated the serum levels of interleukin-6 and VEGF in the model mice.Additionally,histological and immunohistochemical analyses revealed that MSC transplantation systematically improved the morphologies and functions of the liver,kidney,and spleen in T1D model mice.CONCLUSION MenSC transplantation significantly improves the symptoms in T1D model mice and exerts protective effects on their main organs.Moreover,MSC-mediated angiogenesis,antiapoptotic effects and immunomodulation likely contribute to the above improvements.Thus,MenSC are expected to become promising seeding cells for clinical diabetes treatment due to their advantages mentioned above.

滑坡稳定性及其治理方法分析

滑坡是一种地质灾害,是地貌演化的一种方式,本质上是重力流,像岩石碎块或泥土这样的物质在自身重力的影响下从高处向低处滑动,因此,滑坡多发生在山区和丘陵地带。发生滑坡的山体通常比较松散,内部有一些不稳定的层面——滑动面,滑动面在一些诱发因素如降雨的影响下,不稳定层面的黏附力减小从而引发山体滑坡。滑坡会导致河流堵塞,交通阻断,电力通信受损,使人们的财产受损。论文结合工程案例,对潜在性的滑坡稳定性做出了评价并提出了相应的防治措施。

Orexin receptor type 2 agonism inhibits thermogenesis in brown adipose tissue by attenuating afferent innervation

Orexin signaling has been associated with energy expenditure and brown adipose tissue(BAT)function.However,conflicting data exist in the field about how orexin signaling regulates BAT thermogenesis.In this study,we show that a specific orexin receptor type 2(OX2R)agonist[Ala11,D-Leu15]-OxB(OB-Ala)inhibited intrascapular brown adipose tissue(iBAT)thermogenesis by reducing sympathetic output to iBAT.This effect is mediated by OX2Rs located on afferent nerve endings innervating iBAT instead of brown adipocyte itself.Microinjection of OB-Ala into iBAT inhibited iBAT thermogenesis in mice upon cold exposure and neuronal activity in the paraventricular nucleus.Findings suggest that OB-Ala could inhibit iBAT thermogenesis by attenuating sensory input thereby inhibiting the sympathetic-sensory iBAT feedback loop.Our study uncovers a novel primary action site of orexin in the regulation of energy balance.

The Influence of Porous Features on the Electrochemical Performance of Its Supported Platinum Catalyst in Porous Carbon Nanofibers

Porous carbon nanofibers (PCNFs) were prepared through electrospinning, pre-oxidation and carbonization with polyacrylonitrile (PAN) as carbon precursor and polymethyl methacrylate (PMMA), CaCO3 as pore-forming agents. The structure, morphology, specific surface area and electrochemical performance of the carbon nanofibers were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), N2 adsorption/desorption method and electrochemical tests. Compared with PCNFs without CaCO3, PCNFs(CaCO3 1%) had higher specific surface area, better dispersion of Pt nanoparticles, and the particle size become smaller, which was corresponding with the results of electrochemical performance test. It could be seen in cyclic voltammetry (CV) and linear sweep voltammetry (LSV) test, ECSA of Pt/PCNFs (CaCO3 1%) attained 82 m2?g?1, while that of JM20 and Pt/PCNFs without CaCO3 were 77 m2?g?1 and 60 m2?g?1, respectively. These results revealed that CaCO3 as the second pore-forming agent can further increase the mesoporous number and specific surface area of nanofibers, and can improve the electrochemical properties of Pt catalyst as the support.

Nitrogen-Doped Porous Carbon Nanofiber Supported Platinum as Promising Oxygen Reduction Reaction Electrocatalysts

Heteroatoms doped Carbon materials have been proved as promising catalyst support material for oxygen reduction reaction (ORR) in proton exchange membrane fuel cell (PEMFC). In this paper, nitrogen-doped porous nanofibers (N-PCNF) were fabricated via cost effective electrospinning technique by blending the PI and PAN as precursors, followed by heat treatment procedures. The N-PCNFs were used as support to prepare platinum (Pt) catalyst (Pt/N-PCNFs). SEM figure indicated that the porous structures not only existed on the surface but also in the cross session of the fibers. XPS and TEM displayed that with the help of heteroatoms nitrogen, the fiber had rougher surface and more defective structure, contributing to the dispersion of Pt nanoparticles. The catalytic performance for ORR was evaluated by cyclic voltammetry (CV) and liner sweep voltammetry (LSV) with a rotating disk electrode (RDE). According to the results, Pt/N-PCNF exhibited superior property (more positive onset potential and half-wave potential) than that of JM20. The excellent ORR activity of Pt/N-PCNF was attributed to the enriched nitrogen heteroatoms coordinated within the microstructure which increased the exposure of more active sites and dispersion of Pt nanoparticles.

Highly enantioselective construction of CF_(3) -bearing all-carbon quaternary stereocenters: Chiral spiro-fused bisoxazoline ligands with 1,1'-binaphthyl sidearm for asymmetric Michael-type Friedel-Crafts reaction

A novel class of chiral spiro-fused bisoxazoline ligands possessing a deep chiral pocket was prepared.The developed ligands have been employed in the nickel-catalyzed highly enantioselective Michael-type Friedel-Crafts reaction, affording the products bearing a trifluoromethylated all-carbon quaternary stereocenter with moderate to excellent yields(up to 99%) and good to excellent enantioselectivies(up to> 99.9% ee). Moreover, a proposed model of chiral pocket revealed that the attack of indole from the Re-face of β-CF_(3)-β-disubstituted nitroalkene was favorable.

New Sixth-Order Compact Schemes for Poisson/Helmholtz Equations

Some new sixth-order compact finite difference schemes for Poisson/Helmholtz equations on rectangular domains in both two-and three-dimensions are developed and analyzed.Different from a few sixth-order compact finite difference schemes in the literature,the finite difference and weight coefficients of the new methods have analytic simple expressions.One of the new ideas is to use a weighted combination of the source term at staggered grid points which is important for grid points near the boundary and avoids partial derivatives of the source term.Furthermore,the new compact schemes are exact for 2D and 3D Poisson equations if the solution is a polynomial less than or equal to 6.The coefficient matrices of the new schemes are M-matrices for Helmholtz equations with wave number K≤0,which guarantee the discrete maximum principle and lead to the convergence of the new sixth-order compact schemes.Numerical examples in both 2D and 3D are presented to verify the effectiveness of the proposed schemes.