Rapid detection and identification of Escherichia coli(E.coli)is essential to prevent its quickly spread.In this study,a novel fluorescence probe based on ZnTe quantum dots(QDs)modified by mannose(MAN)had been prepare...Rapid detection and identification of Escherichia coli(E.coli)is essential to prevent its quickly spread.In this study,a novel fluorescence probe based on ZnTe quantum dots(QDs)modified by mannose(MAN)had been prepared for the determination of E.coli.The results showed that the obtained QDs showed excellent selectivity toward E.coli,and presented a good linearity in range of 1.0×10~5~1.0×10~8 CFU/mL.The optimum fluorescence intensity for detecting E.coli was found to be at pH 7.0 with a temperature of25℃and incubation time of 20 min.Under these optimum conditions,the detection limit of E.coli was4.6×10~4 CFU/mL.The quenching was discussed to be a static quenching procedure,which was proved by the quenching efficiency of QDs decreased with the temperature increasing.展开更多
基金the grants from National Natural Science Foundation of Guangdong Province(Nos.2017A030310666 and 2018A030307003)Guangdong Medical University Nanhai Marine Biomedical Resources R&D Public Service Platform Open Fund Project(Nos.2HC18013 and 2HC18016)+4 种基金"Group-type"Special Support Project for Education Talents in Universities(No.4SG19045G)Foundation of Young Innovative Talents in Guangdong Province Colleges(No.2018KQNCX091)Undergraduate Science&Technology Innovation Foundation of Guangdong Province(Nos.201810571046 and 201810571073)Medical Science and Technology Development Foundation of Guangdong Province(No.A2016355)The Opening Project of State Key Laboratory of Polymer Materials Engineering of Sichuan University(No.sklpme2018-4-23)。
文摘Rapid detection and identification of Escherichia coli(E.coli)is essential to prevent its quickly spread.In this study,a novel fluorescence probe based on ZnTe quantum dots(QDs)modified by mannose(MAN)had been prepared for the determination of E.coli.The results showed that the obtained QDs showed excellent selectivity toward E.coli,and presented a good linearity in range of 1.0×10~5~1.0×10~8 CFU/mL.The optimum fluorescence intensity for detecting E.coli was found to be at pH 7.0 with a temperature of25℃and incubation time of 20 min.Under these optimum conditions,the detection limit of E.coli was4.6×10~4 CFU/mL.The quenching was discussed to be a static quenching procedure,which was proved by the quenching efficiency of QDs decreased with the temperature increasing.
